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Dr. Bunsen Honeydew Configuration

Suggested voltages current as of Dec 22, 2015.

Detailed information about this instrument can be found in the baseline report (available upon request).

FACSCanto II Configuration:

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

BLUE A

735

780/60

PE-Cy7

550

BLUE B

655

670LP

PerCP-Cy5.5, PE-Cy5.5, PE-Cy5, 7AAD, PerCP

550

BLUE C

610 - - -

BLUE D

556

585/42

PE, PI

400

BLUE E

502

530/30

FITC, GFP, YFP, AlexaFluor 488,

500

BLUE F

-

488/10

SSC

 
BLUE G - -    

BLUE H

---

-

 

 

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

RED A

735

780/60

APC-Cy7

500

RED B

685 -    

RED C

---

660/20

APC, Alexa 647, ToPro3

500

VIOLET 405 LASER (25mW):

Detector

LP

Band Pass

Fluor's

Low Starting Voltage

VIOLET A

502

550/50

Pacific Orange, LiveDead Orange

450

VIOLET B

 

450/50

Pacific Blue, BV 421, DAPI, Vibrant Violet

350

VIOLET C

-

-