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Rowlf Configuration

Suggested voltages current as of 11 March 2013.

Detailed information about this instrument can be found in the baseline report here.

LSRII Configuration: 

BLUE 488 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

BLUE A

735

780/60

PE-Cy7

550

BLUE B

635

695/40

PerCP-Cy5.5, PE-Cy5.5, PE-Cy5, 7AAD, PerCP

550

BLUE C

550 585/42 PE 450

BLUE D

505

530/30

FITC, GFP, YFP, AlexaFluor 430, AlexaFluor 488, TO

450

BLUE E

 

488/10

SSC

 

BLUE F

-

 

 

 
BLUE G - -    

BLUE H

---

-

 

 

RED 633 LASER (20mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

RED A

735

780/60

APC-Cy7

550

RED B

685 660/20 APC, Alexa 647, ToPro3 500

RED C

-

-

 

 

VIOLET 405 LASER (10mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

VIOLET A

505

535/50

Pacific Orange

450

VIOLET B

 

450/50

Pacific Blue, DAPI, Hoechst 33258, Vibrant Violet

350

VIOLET C

-

-