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Scooter - BioRad S3e Configuration

The BioRad S3e is a 2 laser, 4 fluorescent parameter cell sorter capable of two way sorting into 5mL tubes, PCR tubes and 8 well strips using a 100uM nozzle.  Scooter is for use by trained personnel only!

For more information on this instrument click here.

Configuration: 

To design a panel using Fluorofinder please click here.

Collinear Blue 488 (100mW) and Yellow-Green 561nm (100mW) Lasers : Measures two scatter parameters: FSC and SSC. Also measures the following stains:

Detector

Band Pass

Fluor's

FL1

525/30

FITC, GFP

FL2

586/25

PE, dsRed, RFP

FL3

615/25

PE-TxRed, mCherry

FL4 655 LP PE-Cy5, PE-Cy7, mPlum