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Other Services and Charges

Other Services Estimated Time Cost

Rederivation from live mice (IVF)

8 weeks

$1,400

Rederivation from frozen sperms

8 weeks

$1,600

Rederivation from fresh embryos

8 weeks

$1,100

Rederivation from frozen embryos

8 weeks

$1,200

Sperm cryopreservation (including 2 years of storage)

1 week

$1,100

Other Services Cost Minimum Charge

 

Customized CRISPR reagents (including design, template preparation of <200nt ssDNA, and gRNA synthesis and purification)

$800/gene

N/A

Transgenic vector and CRISPR/Cas9 construct in pX330 services (including design, cloning, mini- and large-preparation of plasmid DNA, and confirmation by restriction enzyme digestion or sequencing)

$750 - $1,000/vector

N/A

 

Knockout construct services (including design, cloning, mini- and large-preparation of plasmid DNA, and confirmation by restriction enzyme digestion or sequencing)

 

$1,000 (Knock-in at Rosa26 or CD6 locus, cDNA cost extra)

N/A

$2,500 (knock-in of GFP, tdTomato, lacZ, FLP, Cre, CreER etc. at other locus

N/A

$2,500 (knock-in of other DNA sequences at other locus, cDNA cost extra)

N/A

$3,500 (dual reporter knock-in/conditional knockout)

N/A

Design and test PCR genotyping strategy

$750/allele

N/A

Colony management (including DLAM per diem charge and breeding every 4-6 months)

$1.10/cage day

$25

$800/strain annually

N/A

$1,000/strain annually with PCR genotyping

N/A

Blood sampling/other biopsies

$10/sample (or per tissue)

$50

Tail sampling: < 1 week of age

$5/sample

$50

PCR genotyping (including biopsy and DNA preparation)

$8/sample (one PCR rxn)

$100

$10.50/sample (two PCR rxns)

$100

Male mouse genomic DNA (Southern and PCR quality) (129S6, C57BL/6J, or 129S6/C57BL/6J hybrid)

$50/50 μg

N/A

Mice Cost Processing Fee

C57BL/6J female mice, each

3-4 weeks

$15.50  

$25 per order

5-6 weeks

$17.50

 7+ weeks

$18.50 + $1/week

C57BL/6J male mice, each

3-4 weeks

$12.50

5-6 weeks

$17.50

7+ weeks

$18.50 + $1/week

P0-P7 pups (C57BL/6J and ICR)

$100/litter

$25 per order

Time-mated pregnant C57BL/6 and ICR females (pregnancy guaranteed)


$115.00

$25 per order

CMV-Cre and Rosa26-FLP

$100

$25 per order