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Service Fees

Transgenic Mouse Production

DNA microinjection (transgenic mouse production)

                                                       

C57BL/6J

$2,800

Other Strains

Above fee + mice

Knockout Mouse Production by CRISPR/Cas

 

Project

Estimated Time

Cost

 

 

 

Full service from design to
heterozygotes in C57BL/6J mice with guarantee of germline transmission. (additional mouse cost applies if not in C57BL/6J)

 

 

 

 

*Random indels

5 months

$7,500

*Point mutation(s) within a single exon

5 months

$8,500

*Deletion > 100 bp

5 months

$10,500

*Point mutation(s) in two separate exons

5-10 months

$16,000

Knock-in of small epitope tags (HIS,HA, FLAG, V5, etc.)

5 months

$8,500

Conditional knockout (knock-in of two loxP)

5-10 months

$16,000

CRISPR/Cas9 injection only in C57BL/6J (no guarantee)

Per injection session of PI’s gRNA and template (Cas9 mRNA provided
by the Core) to produce founders

6 weeks

$2,800

Knockout Mouse Production by ES Cell Targeting

 

Project

Estimated Time

Cost

 

 

 

Full service from design to heterozygotes (with
guarantee of germline transmission)

 

 

 

 

 

Knock-in and TetON at Rosa26 or CD6 locus

6 months

$13,000 + cost of
cDNA

Knock-in of GFP, tdTomato, lacZ, FLP, Cre, CreER etc. at other locus

6 months

$15,000

*Knock-in of other cDNA sequences at
other locus

6 months

$15,000 + cost of
cDNA

*Dual reporter (nGFP or tdT) knock-in/ conditional knockout (similar to KOMP/EUCOMM approach)

6 months

$17,000

Knockout/rescue/gain-of-function triple model

6 months

$17,000

Itemized ES Cell Targeting Services

DNA linearization, ESC targeting, DNA preparation and genotyping

4 - 5 weeks

$4,500

Expansion and cryopreservation of ES cell clones

2 weeks

$600 for the 1st clone and $120 each addl clone

Microinjection of KOMP/EUCOMM ESC cells (2 microinjection sessions of up to 3 ESC clones from  a single project) to produce chimeras

6-8 weeks

$5,200