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UR Genomics Research Center

The UR Genomics Research Center (GRC) provides collaborative assistance with experimental design and data analysis for investigators using high-throughput next generation sequencing (NGS), genotyping and gene expression in their research programs. Services include RNA-Seq, Dual RNA-seq, Bar-seq, CHiP-Seq, ATAC-seq, Methyl-Seq, small RNA-Seq, REP-Seq, targeted and whole exome sequencing, whole genome sequencing, 16S rRNA microbiome sequencing, metatranscriptomics, Sanger sequencing, qRT-PCR, Affymetrix microarrays, purification of RNA/DNA, as well as custom applications and approaches. In addition, GRC staff lead collaborative projects with URMC investigators to develop new methodologies and incorporate emerging genomic technologies into faculty research programs and the GRC workflow. Dedicated computational support for hardware, data analysis and storage of high-throughput sequence data is provided by the Center for Integrated Research Computing (CIRC). The following major equipment is located in the GRC: one Illumina HiSeq2500v4 high-throughput DNA sequencer, two Illumina MiSeq DNA sequencers, one C1 Single-Cell Auto Prep System, one 10X Genomics Chromium Controller, one Sage Science Pippin DNA size fractionation system, one QX100 Droplet Digital PCR System, one ABI QuantStudio 12K Flex Real-Time PCR System with autoloader and microfluidic card module, two ABI 9700 PCR machines, two BioRad DNA Engine PCR machines, four BioRad C1000 Thermocyclers, one Eppendorf 5070 liquid handling robot for qPCR setup, one Eppendorf 5075 liquid handling robot for library construction automation, one Covaris S2 sonication system, one Agilent 2200 TapeStation System, two Agilent 2100 Bioanalyzers, one BioRad gel documentation system, one Qubit flourometer and multiple NanoDrop ND-1000 spectrophotometers.