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Instruments

Imaging System

Multiphoton microscope system (Olympus Fluoview FVMPE-RS Twin-Laser Gantry System)

  • The Olympus Fluoview FVMPE-RS is an upright multiphoton microscope tailored for deep-tissue imaging with penetration depth up to 1-1.5mm depending on the sample type.
  • The system is equipped with two two-photon lasers for multi-color imaging
    1. Spectra-Physics InsightX3 Laser (wavelength: 690nm-1300nm)
    2. Spectra-Physics MaiTai HP DeepSee Ti:Sapphire Laser (wavelength: 690nm-1030nm)
  • The system has both galvo and resonant scanners for high spatial and temporal resolution imaging. 
  • Two objectives are currently available for use on this system
    1. Microscope objective Olympus 25x, NA 1.05, for high resolution imaging
    2. Microscope objective Olympus 4x, NA 0.13, for low magnification navigation

Multiphoton Imaging System

Surgical Dissecting Scope

Two Leica surgical dissecting scopes are available at the core for animal preparation before surgery.

Optical, Opto-Electronical, & Opto-Mechanical Equipment

  • Near-IR-Spectrometer (PSPECNIR, Newport)
  • Laser-power meter with high power and low power detector (1918-C, Newport)
  • Collection of lenses, optical fibers, dichroic mirrors and emission filters (Semrock, Chroma, Newport, Thorlabs)
  • Collection of opto-mechanical components (Linos, Newport, Thorlabs)

Equipment for Electrophysiology & Systems Physiology

  • Picoammeter for polarographic oxygen measurements
  • 16 channel-real time data acquisition system (Digidata 1440/pCLAMP10, Axon Instruments)
  • Transonic System laser Doppler flow meter
  • World Precision Instruments pressure monitor BP-1
  • Kent VetFlo isoflurane vaporizer for small animal anesthesia during imaging
  • Kent SomnoSuit isoflurane vaporizer for small animal anesthesia during surgery

Using the Instruments

The Multiphoton Core Facility is organized around the principle of providing researchers at the URMC and the University of Rochester with access to and training on the state-of-the-art multiphoton system and image analysis tools. If you are interested in learning about our core facility or planning to use the Multiphoton Core Facility in your research, please contact the core director for consultation on experimental design, input on letters of support and descriptions of equipment, as well as potential collaborations. We are devoted to facilitating researchers with high quality quantitative imaging, and enhancing your chances of successful funding.

Please follow steps below if you are interested in using the two-photon microscope:

Step 1. Contact Dr. Yurong Gao to schedule a one-on-one consultation to discuss the image setup and experiment goals.

Step 2. Create a PPMS account by completing the New User Registration Form to be able to access system calendar (if you don't have PPMS account).

Step 3. Complete the required laser safety training on Blackboard or Mypath: Lab Safety Training.

Step 4. Learn multiphoton microscopy basics through online video tutorial: https://www.ibiology.org/talks/two-photon-microscopy/

Step 5. Schedule a 4hours hands-on microscopy training with Dr. Yurong Gao. This initial training covers basics of the microscope including both hardware and software. Test slide is provided by the core for practice. Samples prepared by users are welcomed.

Step 6. Advanced hands-on training can be requested based on the user’s needs.  Prior to this advanced training, the user must be able to perform basic functions on the system independently. 

Step 7. Once the user has used the system for at least 20 hours within previous 4 months independently, the user will be granted Autonomous access to the system to image after business hours.