2:00pm - 3:30pm|
Imaging Mass Spectrometry: Molecular Mapping Beyond the Microscope
Presented by Dr. Richard Caprioli, Stanley Cohen Professor of Biochemistry and Director of Mass Spectrometry Research Center, Vanderbilt University School of Medicine
MALDI Imaging MS produces molecular maps of peptides, proteins, lipids and metabolites present in intact tissue sections. It employs desorption of molecules by direct laser irradiation to map the location of specific molecules from fresh frozen and formalin fixed tissue sections without the need for target-specific reagents such as antibodies. Dr. Caprioli has employed Imaging MS in studies of a variety of biologically and medically relevant research projects. One area of special interest is the molecular mapping of changes observed in diabetes in both a mouse model and in the human disease. This presentation will focus on recent technological advances both in sample preparation and instrument performance to achieve images at high spatial resolution (1-10 microns) and at high speeds so that a typical sample tissue, once prepared, can be imaged in just a few minutes. Finally, new biocomputational approaches will be discussed which deal with the high data dimensionality of Imaging MS and our implementation of 'image fusion' in terms of predictive integration of MS images with microscopy and other imaging modalities.
Contact Mark Platt, Ph.D., Director, URMC Proteomics Resource Facility for information (585) 276-6804.
|Location: ||K 207 Auditorium (2-6408)|
Posted by: Anne Reed, Shared Resource Laboratories, 9-Apr-14 8:33am ET