Needle Muscle Biopsy

The following file describes the procedure used by the Field's Center to collect muscle biopsy research samples, and provides information on the types of needles and suppliers (updated 10/2013): ( Download PDF file).


Background: Needle muscle biopsy sampling offers a less invasive, more rapid alternative to conventional open muscle biopsies for sampling muscle for research purposes. The smaller size of the sample obtained is no longer an important limitation as the sensitivity of emerging molecular technologies has improved exponentially and small amounts of substrate can go a long way.  Using the sampling techniques described below, up to 300 mg of tissue, depending on muscle bulk, can be obtained with three passes into the muscle.  Such a sample, divided into three, is more than adequate for flash freezing in liquid nitrogen for expression studies, placement in appropriate media for myoblast culture as well as for histologic studies.


For more information, please contact the Fields Center