Welcome to URMC Flow Cytometry Resources

The mission of URMC Flow Cytometry Core is to provide investigators with state-of-the-art instrumentation along with the human expertise to support all that is possible now, while pushing the limits of what can be done with flow cytometry.

The Core currently has analytical tools including a FACSCanto II (8-colors) and 5 LSR-IIs (two 12-color, two 18-color, and one 8-color instrument) from BD Biosciences. We also have a 13-color BD FACSAria IIu cell sorter, an 18-color BD FACSAria II cell sorter, the Amnis ImageStream GenX, and two BD Accuri C6 cytometers located in the CVRI and the Flow Core.

Instrumentation is only part of what we offer. In close association with the Rochester Human Immunology Center, we also offer comprehensive training for your staff, consultation for maximizing the effectiveness and cost of your experiments, as well as an environment that fosters success in both existing methods and crossing the boundaries into new frontiers.

Flow core staff are available for consultation and troubleshooting of experiments. Additionally, during the spring and fall semester, the FCC hosts a weekly lecture series focusing on important educational topics such as data analysis and statistics, experimental design and validation, and specialized topics like DNA analysis, intracellular staining and imaging flow cytometry. The FCC also hosts an annual meeting in cytometry, the Western NY Flow Cytometry Users group, held in July.

To access the FlowJo licensing server, use the following address: http://www.flowjo.com/FLS/registration/urmc.html

Additional FlowJo server information can be found in the library.

New and Noteworthy

19 April 2013

The server is back online! Connecting to the transfer space from any instrument is the same as before - user the shortcut on the desktop. To connect from your lab, there are new instructions in the Library. Please be sure to read!

Also, the instrument computers no longer have individual usernames listed. There is now a generic login for each instrument. To log in, just press OK, there is no password for the generic accounts.

15 March 2013

Colleagues,

The Shared Resarch Laboratories (SRL's) are in the process of transitioning to a unified web-based system for instrument reservations, usage tracking, service work and invoicing. The new system is PPMS by Stratocore. When PPMS is fully implemented, each user of any of the URMC SRL's will have their own home page under one central URL to service their research facility needs.

The initial roll out will be for the Flow Cytometry Core (FCC). All current usernames and passwords have been transferred to the PPMS system. You may now go to https://ppms.info/urmc/ and log in to the site. If you have not changed your password from the originally assigned default, you will be required to do so at first login. We would like to encourage everyone to log in to the PPMS system and preview the features over the next couple weeks. The system will then "go live" on April 1.

Reservations for time/use through March 31 should be made on the current calendar(using your current password). Reservations for time/use on or after April 1 should be made on the new calendar. Any existing reservations for April 1 and beyond have been transferred to the new calendar.

To reserve time with PPMS, go to your home page, select the instrument you wish to reserve from the dropdown under "Book a system", select the time/date you want by checking the appropriate boxes, scroll to the bottom and click "book the selected sessions". If you use the account number drop down on the old calendar, you will find the account names you are eligible to use under the Project dropdown, simply select the "project" before you book.

When you go to use the instrument, you will find a gray screen, with boxes for user name and password. If you currently have a password for the instrument, login with your PPMS credentials, and the system will become available. Otherwise, get someone from the facility staff to log you in, as you do now. When you are finished, be sure to click the logout button in the lower right of the screen.

Further instructions for other features of the system will soon be available on our website.

If you have any questions, please do not hesitate to contact us:

Matt Cochran 3-4020
Dave Fuller 6-6437
Tim Bushnell 3-553

13 March 2013

A new lot of CS&T beads has arrived and all the instruments have been updated to reflect the new baselines and Peak 2 values. Instrument pages have been updated accordingly.

8 January 2013

Happy New Year! May it be filled with flow cytometry and good tidings for all.

All news from the past year has been moved to the archives. If you are looking for something, head here.

Looking for old news? The archives are here.

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The URMC Flow Core sincerely thanks the Clinical And Translational Science Institute’s (Grant number UL1 RR024160) for their considerable and continued support of mission critical equipment. http://www.urmc.rochester.edu/ctsi

Acquisition of the Amnis ImageStream was made possible by NIH Grant S10 RR0023458, (Craig Jordan, PI).

The URMC Flow Cytometry Core sincerely thanks the UR Developmental Center for AIDS Research (P30AI078498, NIH/NIAID) for their support in the acquisition of the Amnis ImageStreamX.

The URMC Flow Core would also like to thank the Rochester Human Immunology Center for their support of mission critical equipment.

The URMC Flow Core is grateful to NCRR for the funding of a new Aria II high speed cell sorter in part from grant number 1S10RR0292299-01.