Welcome to URMC Flow Cytometry Resources

The mission of URMC Flow Cytometry Core is to provide investigators with state-of-the-art instrumentation along with the human expertise to support all that is possible now, while pushing the limits of what can be done with flow cytometry.

The Core currently has analytical tools including twoFACSCanto IIs(6- and 8-colors) and 4 LSR-IIs (two 12-color and and two 18-color instruments) from BD Biosciences. We also have a 13-color BD FACSAria IIu cell sorter, an 18-color BD FACSAria II cell sorter, an Amnis ImageStream Imaging Cytometer, the Amnis ImageStream GenX, and a BD Accuri C6 cytometer located in the CVRI.

Instrumentation is only part of what we offer. In close association with the Rochester Human Immunology Center, we also offer comprehensive training for your staff, consultation for maximizing the effectiveness and cost of your experiments, as well as an environment that fosters success in both existing methods and crossing the boundaries into new frontiers.

Flow core staff are available for consultation and troubleshooting of experiments. Additionally, during the spring and fall semester, the FCC hosts a weekly lecture series focusing on important educational topics such as data analysis and statistics, experimental design and validation, and specialized topics like DNA analysis, intracellular staining and imaging flow cytometry. The FCC also hosts an annual meeting in cytometry, the Western NY Flow Cytometry Users group, held in July.

To access the FlowJo licensing server, use the following address: http://www.flowjo.com/FLS/registration/urmc.html

Additional FlowJo server information can be found in the library.

If you are a registered Flow Core user and need access to the 3-4100 hallway, please fill out the following form.

New and Noteworthy

16 May 2012

With the holiday on May 28th, our training lecture was canceled. We have booked the same room, and same time, for training the day after, Tuesday May 29th!

14 May 2012

For those interested, there is an excel sheet highlighting the differences between our previous and new bead lots. It can be found from the Help Desk link regarding Peak 6 informaiton.

Also to note, out ninth annual meeting is coming up in July! If you would like to register, please use our registration link here. More information about the meeting can also be found at the link to the left.

7 May 2012

The next training lecture is May 14th. There will not be another training lecture in May as the 28th is a holiday. If there is a need for training before June, please let us know and we will try to accomodate!

4 May 2012

All new baselines have been run on our herd of Muppets. New Peak 2 voltages have also been run, and websites have been updated.

2 May 2012

To echo Tim's email sent earlier today about sorting:

Colleagues:

As a reminder from our April 13th email to the list, we have implemented a
new sort form request that MUST be filled out and APPROVED before sorting.

On May 7th, if a sort has not been approved, the sort will not be performed
and you will be billed for the time reserved.

The form that Tim speaks of can be found here. Please be sure you are familiar with this form, and if you are not please contact either Tim or Matt to discuss the issues you are having.

23 April 2012

After all the work Fozzie had done last week, there was a need to redo his Peak 2 voltages. This information is now posted on his instrument page, and the new baseline is also in the library if needed.

13 April 2012

Over the weekend, our new database and sorting system will be rolled out. Please be sure to read the cytometry listserv announcement that Tim sent out earlier today. As always, feel free to stop by or let us know if you have any issues.

29 March 2012

With the recent work on Dr Bunsen, his voltages have been updated and the new baseline is available for some light reading in the library or from his instrument page.

26 March 2012

There is now an Accuri training document located on the library page of the website.

19 March 2012

The library page has been updated slightly to provide a question guided approach to locating information on the website. If there is something you routinely have to hunt for, please let a staff member know so the page can be updated!

2 March 2012

The next two training lecture dates have been posted. This coming Monday is the soonest. Also, there are two new instrument guides in the Library. One for FACSDiva, and one for the ImageStream. Give them a read if you are hungry for information!

6 February 2012

In an effort to streamline the data on intrument configuration pages, the linear ranges of PMTs has been removed and a link to each instrument's baseline report can now be found on the top of it's page.

3 February 2012

After a long delay, the seminar from January 9th is now available on the lectures page.

Also, two new forms have come online. The Change of Information form is for use then information tied to your account has changed. This would be account numbers, labs you work in, or if you are leaving the university. The second form is a Data Recovery Request. This form is to be used if you wish to recover data older than two months since it will no longer be on the local machines. Please fill out that form and the rest will be take care of. As always if you have any comments please feel free to contact a staff member. Thanks!

9 January 2012

Tuesday Tuesday Tuesday! Tomorrow in K-207 the Flow Core hosts the next seminar "Multicolo Flow Cytometry" with guest speaker Darci Gorgone from BD Biosciences.

14 December 2011

There was a slight case of confusion with the voltages on Animal and Fozzie. This has been fixed and each instrument now has the correct numbers on their page.

7 December 2011

The next lecture presented by the Flow Core will be on Tuesday January 10th in K-207. Check the Lectures page for the abstract of this talk.

6 December 2011

With baselines being redone on all the instruments, new suggested voltages will also be present. These pages are located on the instruments themselves and will also be posted on the website shortly.

22 November 2011

Notice to all invesitgators that sort:

There is now a place in your reservation to fill in what nozzle you need for your sort. Please be sure to have this information filled in for future visits to best assist us with preparing for your arrival. The guideline for this new policy can be found on the sorting information page.

14 November 2011

Dearest Users,

The training dates through the end of the year have been posted. Please feel free to contact us if you have any questions about the dates listed. Thanks!

10 November 2011

As a note to all FlowJo users:

The price of dongles and dongle upgrades will be going up as of Jan 1st. Please be aware of this and see the following address for information on the price increase.

Old prices: http://www.flowjo.com/home/pricing.html

New prices: http://www.flowjo.com/home/prices2012.html

Looking for old news? The archives are here.

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The URMC Flow Core sincerely thanks the Clinical And Translational Science Institute’s (Grant number UL1 RR024160) for their considerable and continued support of mission critical equipment. http://www.urmc.rochester.edu/ctsi

Acquisition of the Amnis ImageStream was made possible by NIH Grant S10 RR0023458, (Craig Jordan, PI).

The URMC Flow Cytometry Core sincerely thanks the UR Developmental Center for AIDS Research (P30AI078498, NIH/NIAID) for their support in the acquisition of the Amnis ImageStreamX.

The URMC Flow Core would also like to thank the Rochester Human Immunology Center for their support of mission critical equipment.

The URMC Flow Core is grateful to NCRR for the funding of a new Aria II high speed cell sorter in part from grant number 1S10RR0292299-01.