Principal Investigator

Elizabeth Grayhack, Ph.D. University of Rochester work Box 712 Rochester NY p 585-275-2765 f 585-275-6007

Research Projects

  • The Role of the Genetic Code in Translation in Saccharomyces cerevisiae

    The Role of the Genetic Code in Translation in Saccharomyces cerevisiae

    A major focus in the laboratory is to understand how codon choice influences translation efficiency in a eukaryote. Although codon choice has been associated with large disparities in translation efficiency for many years, it is still unknown the extent to which codon choice directly influences translation efficiency, as well as the identity and properties of the codon-initiated signals. We think codon-mediated regulation of expression is likely to be functionally important and nearly universal, as all organisms require wobble decoding of some codons and since clusters of rare codons are frequently conserved between orthologous genes in humans and chimps. More info...

  • Functional Proteomics

    Functional Proteomics

    The wealth of sequenced genomes has resulted in the development of powerful genomic approaches to define the functions of the individual genes, to deduce the networks of interactions between genes and to mine additional information encoded in the genome. For example, a collection of 5,854 sequence-verified yeast expression plasmids opened the door to study signals within genes that regulate their expression, as well as to develop yeast as a host for expression of proteins for biochemical and structural analysis. More info...

  • Genomic Analysis of Fitness in Oral Bacterium Streptococcus mutans

    Genomic Analysis of Fitness in Oral Bacterium Streptococcus mutans

    Analysis of the phenotypic effects of gene disruptions has been a powerful method to evaluate the contributions of individual genes to fitness in particular conditions. To assess the contributions of each gene in the oral pathogen S. mutans to virulence, we are constructing a marked collection of strains each bearing a deletion of a single gene. More info...