Pre-mRNA Splicing for Treatment of Human Disease
Most human cancers, leukemia’s and many metabolic disorders are accompanied by severe defects in pre-mRNA splicing. RNA splicing is a key step of gene expression and is essential for human life. A major focus of my laboratory is to understand at a molecular level how the splicing machinery identifies sites for excision from gene transcript RNAs, which in turn changes the proteins produced. We have characterized the three-dimensional shapes of human splicing proteins recognizing one another and the gene transcript RNA [for example, Kielkopf et al. (2001); Kielkopf, Luecke, Green (2004); Sickmier et al. (2006); Gupta, Jenkins, Kielkopf (2011)] (shown above), both at high resolution by X-ray crystallography and in solution by small-angle X-ray scattering and nuclear magnetic resonance. Through this work, we identify a network of interactions responsible for recognizing human splice sites.
Currently, our major goals investigate:
- The three-dimensional shapes of the full assembly of human proteins bound to the gene transcript
- The interactions among Aspergillus fumigatus splicing factors, a soil fungus that frequently causes pulmonary infections in AIDS patients.
- Function and potential of splicing factor phosphorylation to serve as a drug target
Altogether, this work would elucidate:
- How pre-mRNA splicing fidelity is ensured in humans
- How pre-mRNA splice site recognition is achieved in normal cells and altered in cancers and genetic disease
- Differences between Aspergillus and human splicing factors for targeting by small molecule therapeutics
Current funding includes R01 GM070503 (Kielkopf):
Molecular recognition during pre-mRNA splicing and F32 GM097916 (Laird):
Molecular Means for 3' Splice Site Recognition by U2AF35.
- Staufen1 dimerizes through a conserved motif and a degenerate dsRNA-binding domain to promote mRNA decay. Nat Struct Mol Biol. 20, 515-24. (2013 Apr 01).
- U2AF65 adapts to diverse pre-mRNA splice sites through conformational selection of specific and promiscuous RNA recognition motifs. Nucleic Acids Res. 41, 3859-73. (2013 Apr 01).
- Structure of phosphorylated SF1 bound to U2AF⁶⁵ in an essential splicing factor complex. Structure. 21, 197-208. (2013 Feb 05).