Principal Investigator

Clara L. Kielkopf, Ph.D. University of Rochester work Box 712 601 Elmwood Ave Rochester NY 14642 p (585) 273-4799 f (585) 275-6007

Research Projects

  • Polypyrimidine Tract Recognition By U2AF65

    Polypyrimidine Tract Recognition By U2AF65

    The uridine-rich sequence by the 3’ splice of the pre-mRNA is recognized by the U2 snRNP auxiliary factor large subunit, U2AF65. We have shown how U2AF65 can distinguish uridines - by specific hydrogen bonds with the base edges. More info...

  • Phosphorylation of Splicing Factor-1

    Phosphorylation of Splicing Factor-1

    Splicing factor-1, SF1 recognizes a branch point sequence (BPS) consensus preceding the 3’ splice site and is regulated by phosphorylation. We are investigating the structural and functional consequences of phosphorylation for human SF1 interactions with U2AF65 and the pre-mRNA splice site. More info...

  • Pre-mRNA Recognition by U2AF35 Heterodimer

    Pre-mRNA Recognition by U2AF35 Heterodimer

    The small subunit of the U2 snRNP auxiliary factor heterodimer, U2AF35, specifically contacts the 3’ splice site junction during pre-mRNA splicing. Whether U2AF35 contributes to the RNA affinity and specificity of the U2AF heterodimer, or is passively positioned to contact the RNA by U2AF65, is an outstanding question that we are currently seeking to address. More info...