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Center for Musculoskeletal Research

URMC / Center for Musculoskeletal Research / Core Services / Histology, Biochemistry, and Molecular Imaging (HBMI) Core / Facilities

 

HBMI Core Equipment and Facilities

The HBMI Core is located in rooms 1.7564, 1.7574, 1.7531 of the Center for Musculoskeletal Research on the University of Rochester Medical Center campus. 

Histology, IHC and ISH Sub-Core

IHC ISH Sub-CoreThe Histology, IHC, and ISH Sub-Core provides resources and support for the generation of tissue sections from paraffin and frozen embedded tissue samples and the subsequent histological, IHC, and ISH analyses. Major equipment includes:

  • Sakura Tissue-Tek VIP 6 automated histoprocessor (1)
  • Sakura Tissue-Tek TEC 5 paraffin embedding center (2)
  • Microm HM355S automated microtome (3)
  • Leica RM2255 automated microtome (4)
  • Two Microm HM315 microtomes & one Microm HM325 microtome (5)
  • Leica CM1850 cryostat with a CryoJane tape transfer system (6)
  • Leica CM1860UV cyrostat (7)

Microscopy, Histomorphometry, and Imaging Sub-Core

The Microscopy, Histomorphometry, and I Imaging Sub-Core provides resources and support for the generation of digital bright field, dark field, and fluorescent imaging of tissue or cell preparations and the subsequent quantitative and histomorphometric image analyses.

Olympus VisiopharmMajor equipment includes:

  • Olympus VS120 Virtual Slide Microscope and Visiopharm Image Analysis System (1)
    The system features include:
    • 100 slide capacity with a dependable and durable slide loader
    • Diagnostic quality images produced using 2x, 10x, 20x or 40x objective lenses
    • 2/3” CCD camera (3.45 μm x 3.45 μm pixel size, high sensitivity, high resolution)
    • Macro image for complete overview of the glass slide
    • Entire slide scan or a region of interest scan
    • Fluorescence filters included are FITC; Texas Red; Triple with FITC, Texas Red, & DAPI, with reduced bleaching problems in fluorescence slides
    • Z-plane focus available on Brightfield and Fluorescence slides
  • Microscopy, Histomorphometry and Imaging Sub-CoreTwo Zeiss Axioskop 40 BF/DF/fluorescent microscopes with SPOT RT3 color/slider cameras and dedicated computer and software packages (2)
  • Olympus SZX12 Stereoscope with a SPOT Flex color camera (3)
  • Zeiss AxioImager motorized fluorescent microscope system with AxioCam camera and dedicated computer and software (4)
  • Zeiss Axiovert 40 CFL fluorescent inverted microscope with AxioCam camera and dedicated computer and software (5)
  • Olympus BH2 light and fluorescence microscope with an attached digital camera and OsteoMetrics, Inc. histomorphometry hardware and OsteoMeasureXP software (v3.2.1.8, purchased in 2011) (6)