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James Corsetti, M.D., Ph.D.

Associate Professor of Pathology and Laboratory Medicine

Clinical Chemistry
Regulation of Hepatic Metabolism of Lipoproteins

URMC Labs
601 Elmwood Ave., Rm G-1572
Rochester, NY 14642-8608
Tel: (585) 275-4907
Fax: (585) 273-3003

James_Corsetti@urmc.rochester.edu

Dr. Corsetti is Medical Director for laboratory specimen management and Associate Director of the Protein Laboratory.

Qualifications

M.D., Brown University 1982
Ph.D., Physical Chemistry, Harvard University 1980
Resident in Pathology, University of Rochester Medical Center 1982-86
Board Certification (AP/CP), American Board of Pathology 1987

Research Overview

Our work focuses on the role of the regulation of hepatic metabolism of lipoproteins especially as related to regulatory alterations associated with atherogenesis. A major effort in this laboratory involves studies of the role of biochemical hepatocellular heterogeneity in the metabolism of lipoproteins. Studies to date, especially utilizing flow cytometry, have demonstrated cellular heterogeneity of LDL uptake by rat liver through the use of preparations labeled with a fluorescent probe developed in our laboratory for this purpose, and cellular heterogeneity in apo B expression in rat liver by intracellular immunocytochemical fluorescence techniques. These studies provide basic information regarding regulation of lipoprotein metabolism in the liver but, more directly, they suggest the notion that recruitment of hepatocellular subpopulations that are normally quiescent with regard to lipoprotein metabolism could be helpful for normalization of aberrant plasma lipoproteins. We are extending these studies using plasma membrane markers of hepatocyte heterogeneity to further characterize alterations in metabolism at the molecular level. Additional projects in our laboratory include characterization of a newly developed rat model of NIDDM, the Zucker diabetic fatty (ZDF) rat, especially as related to gender differences in regard to the development of NIDDM in response to dietary fats; and studies of possible roles of cellular heterogeneity among circulating leukocytes, especially monocytes, in atherogenesis. se.

Publications

Sparks JD, Shaw WN, Corsetti JP, Bolognino M, Pesek JF, Sparks CE. Insulin-treated Zucker diabetic fatty rats retain the hypertriglyceridemia associated with obesity. Metabolism. 2000 Nov;49(11):1424-30.

Chirieac DV, Chirieac LR, Corsetti JP, Cianci J, Sparks CE, Sparks JD. Glucose-stimulated insulin secretion suppresses hepatic triglyceride-rich lipoprotein and apoB production. Am J Physiol Endocrinol Metab. 2000 Nov;279(5):E1003-11.

Corsetti JP, Sparks JD, Peterson RG, Smith RL, Sparks CE. Effect of dietary fat on the development of non-insulin dependent diabetes mellitus in obese Zucker diabetic fatty male and female rats. Atherosclerosis. 2000 Feb;148(2):231-41.

Sparks JD, Phung TL, Bolognino M, Cianci J, Khurana R, Peterson RG, Sowden MP, Corsetti JP, Sparks CE. Lipoprotein alterations in 10- and 20-week-old Zucker diabetic fatty rats: hyperinsulinemic versus insulinopenic hyperglycemia. Metabolism. 1998 Nov;47(11):1315-24.

Lazova RZ, Sterry JA, Corsetti JP. Pseudoparaproteinemia due to hemoglobinopathy. Clin Chem. 1995 Sep;41(9):1321-2. No abstract available.

Sparks JD, Corsetti JP, Sparks CE. Liver regrowth and apolipoprotein B secretion by rat hepatocytes following partial hepatectomy. Metabolism. 1994 Jun;43(6):681-90.

Corsetti JP, Cox C, Schulz TJ, Arvan DA. Combined serum amylase and lipase determinations for diagnosis of suspected acute pancreatitis. Clin Chem. 1993 Dec;39(12):2495-9.

Corsetti JP, Sparks JD, Sikora B, Sparks CE. Cellular heterogeneity in binding and uptake of low-density lipoprotein in primary rat hepatocytes. Hepatology. 1993 Apr;17(4):645-50.

Corsetti JP, Way BA, Sparks CE, Sparks JD. Immunolocalization, quantitation and cellular heterogeneity of apolipoprotein B in rat hepatocytes. Hepatology. 1992 Jun;15(6):1117-24.

Corsetti JP, Sterry JA, Sparks JD, Sparks CE, Weintraub M. Effect of weight loss on serum lipoprotein(a) concentrations in an obese population. Clin Chem. 1991 Jul;37(7):1191-5.