Trevor J. Shuttleworth, Ph.D.

Trevor J. Shuttleworth, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 711
Rochester, NY 14642

Fax: (585) 273-2652
Lab: (585) 275-3431
Office: (585) 275-2076

Research Bio

The cellular actions of many neurotransmitters and hormones are dependent on receptor-activated increases in intracellular calcium concentrations in their target cells. In various secretory and other "non-excitable" cells, the physiologically relevant signals often take the form of a series of Ca2+ oscillations, and many critical cellular responses are tuned to respond to the specific frequency of such oscillations. A key factor in sustaining these signals and determining their frequency, is the receptor-activated entry of Ca2+ from the extracellular medium. Our research concerns the nature of the conductances responsible for this entry, and their specific roles in modulating the overall Ca2+ signal. Specifically, our recent research has focused on the ARC channel – a novel Ca2+ entry channel that is activated by the receptor-mediated generation of arachidonic acid, and which we first described a few years ago. Importantly, it is this channel that appears to play the major role in the entry of Ca2+ during the generation of the oscillatory Ca2+ signals in several cell types. We have recently characterized the key molecular components of this channel, revealing that it is a member of a new family of channels – the "Orai-based channels". This molecular characterization has opened up new opportunities for the analysis of its nature, mechanism of activation, and regulation. This, along with identifying how the Ca2+ entering via this channel acts to modulate the frequency of the agonist-induced Ca2+ oscillations, forms the major focus of our current research.



Techniques used include patch-clamp analysis of ion-channel activity, digital imaging, real-time confocal imaging, and photon-counting microfluorimetric measurements of intracellular Ca2+ concentrations in single cells, and biochemical and molecular studies of the proteins that form the channel and its regulators.

Awards & Honors (Local)

Chair and Organizer, Satellite Symposium on "Orai Channels--New Insights, New Ideas" | 56th Annual Biophysical Society Meeting | San Diego, California 2012
Plenary Speaker, Ion Channel Signaling Mechanisms: from Basic Science to Clinical Applications | Marrakesh, Morocco 2011 - 2011
Session Chair - Salivary Glands and Saliva | Gordon Research Conference | Ventura, California 2003
Session Chair - 5th UK Calcium Signalling Conference, Liverpool, England. 2003
Session Chair - Professor Sir Michael Berridge Retirement Conference, Cambridge, England. 2003
Session Chair - International Workshop on "Experimental and Theoretical Calcium Dynamics" Max-Planck institute for the Physics of Complex Systems, Dresden, Germany 2000
Session Chair - Experimental Biology 1993 (FASEB) meeting, New Orleans 1993
Session Chair - Joint Meeting of the APS, ASZ, CSZ and SEB, Cambridge, England 1992
Session Chair - International Symposium on "Mechanisms of Systemic Regulation in Lower Vertebrates", Göttingen, Germany 1990
Session Chair - Society for Experimental Biology Conference, York, U.K. 1987
Session Chair - IUPS International Congress, Vancouver, Canada 1986
Session Chair - IUPS Satellite Symposium on "Membrane Transport and Control: Comparative Mechanisms", Banff, Canada 1986
Session Chair - Society for Experimental Biology Conference, Bangor, U.K. 1985
Session Chair - Society for Experimental Biology Conference, Cardiff, U.K. 1984
Session Chair - Seminar Series Symposium of the Society for Experimental Biology, Durham, U.K. 1981
Session Chair - Society for Experimental Biology Conference, Lancaster, U.K. 1979
Science Research Council Fellowship (U.K.), University of Lancaster, England 1972 - 1974
University of Otago Teaching Fellowship, University of Otago, New Zealand 1969 - 1972

Recent Journal Articles

Showing the 5 most recent journal articles. 100 available »

2013
Thompson, JL; Shuttleworth, TJ. "How many Orai's does it take to make a CRAC channel?". Nature Scientific Reports. 2013; 3: 1961.
2013
Thompson, JL; Shuttleworth, TJ. "Molecular basis of activation of the arachidonate-regulated ARC channel, a store-independent Orai channel, by plasma membrane STIM1". The Journal of Physiology. 2013; 591: 3507-3523.
2012 Sep 1
Shuttleworth TJ. "Orai channels - new insights, new ideas." The Journal of physiology.. 2012 Sep 1; 590(Pt 17):4155-6.
2012 Jul 21
Palk L, Sneyd J, Patterson K, Shuttleworth TJ, Yule DI, Maclaren O, Crampin EJ. "Modelling the effects of calcium waves and oscillations on saliva secretion." Journal of theoretical biology.. 2012 Jul 21; 305:45-53. Epub 2012 Apr 14.
2012 Jan 15
Shuttleworth TJ. "Orai3--the 'exceptional' Orai?" The Journal of physiology.. 2012 Jan 15; 590(Pt 2):241-57. Epub 2011 Oct 31.

Current Appointments

Professor - Department of Pharmacology and Physiology (SMD) - Primary
Professor - Center for Oral Biology

Education

PhD | Zoology/Comp. Physiology | New Zealand-Univ of Otago1972
BS | Zoology | UK-Univ of London1968

Post-Doctoral Training & Residency

SRC Research Associate, Department of Biological Sciences, University of Lancaster, England 1974