Denise C. Hocking, Ph.D.

Denise C. Hocking, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 711
Rochester, NY 14642

Office: (585) 273-1770
Fax: (585) 273-2652
Lab: (585) 273-1771

Research Bio

Research in the Hocking lab focuses on understanding the mechanisms by which the extracellular matrix protein, fibronectin, affects cell and tissue functions that are critical for wound repair. We study both the structural mechanisms and intracellular signaling events that mediate cell and tissue responses to matrix fibronectin. In turn, we are using this information to develop novel technologies for tissue engineering, and therapeutic approaches to promote tissue regeneration in chronic wounds.



The Extracellular Matrix: What is it? The extracellular matrix (ECM) is a complex, three-dimensional network comprised of collagens, glycoproteins, and proteoglycans that provides an adhesive substrate for the organization of cells into tissues. In the adult, dynamic interactions between cells and their surrounding ECM proteins regulate most, if not all, cell and tissue functions. ECM proteins also contribute to the mechanical and permeability properties of the skin, vasculature, lungs, and other organs. Fibronectin is a principal component of the ECM, where it is organized into elongated, branching fibrils. Mechanical forces influence the deposition, organization, and structure of ECM fibronectin fibrils, which in turn, affect cell function, ECM organization and stability, vascular perfusion, blood vessel permeability, and tissue strength. Fibronectin is initially secreted by cells in a soluble, protomeric form. Soluble fibronectins are then polymerized into ECM fibrils by means of a tightly-regulated, cell-dependent mechanism that can be rapidly up- or down-regulated. Fibronectin matrix polymerization is increased in response to tissue injury; altered fibronectin matrix deposition is associated with abnormal tissue repair in a number of chronic inflammatory states, including non-healing wounds, asthma, pulmonary fibrosis, and atherosclerosis. In the body, fibronectin polymerization is a continuous process, with as much as 50% of the fibronectin matrix undergoing turnover every 24 hours. In spite of this remarkable information, the mechanisms of fibronectin matrix polymerization and the function of ECM fibronectin fibrils in vivo is not well understood.

Studies from the Hocking Lab: What have we learned about fibronectin? Research in the Hocking lab has been directed at determining whether and how this three-dimensional fibronectin matrix regulates cell behaviors that are essential to tissue repair. Our studies have firmly established that cells respond differently to soluble versus ECM fibronectin, and that the ECM form is the primary functional form of fibronectin. We have shown that the conversion of fibronectin into the ECM form specifically stimulates cell spreading, cell migration, and collagen matrix contraction – all cellular events critical to tissue regeneration. We have also shown that the assembly of a fibronectin matrix promotes the co-polymerization of collagen I and enhances the tensile strength of model tissue, thus demonstrating that the organization and functional properties of the ECM are also dependent on fibronectin matrix polymerization. We have localized several of the ECM-specific effects of fibronectin to a single, cryptic heparin-binding site in the first type III module of fibronectin (FNIII-1). This conformation-dependent, or "matricryptic" site is not exposed in soluble fibronectin, but becomes unmasked during fibronectin matrix formation or as cells and tissues exert tension on ECM fibronectin fibrils. Our exciting new studies, conducted in collaboration with the laboratory of Dr. Ingrid Sarelius, demonstrate that exposure of this matricryptic site in vivo mechanically couples skeletal muscle contraction with local vasodilation. These were the first studies to demonstrate a role for fibronectin fibrils in adult connective tissue in vivo.

Awards & Honors (Local)

Proctor and Gamble Career Opportunity Award, American Physiological Society. 1990

Patents

Ultrasound Technology to Control the Spatial Organization of Cells and Proteins in Engineered Tissues

United States Serial NO.: 13/321,218
Filed Date: April 15, 2010
Title: Ultrasound Technology to Control the Spatial Organization of Cells and Proteins in Engineered Tissues
Invented by: Diane Dalecki, Denise Hocking, Kelley Garvin
Fibronectin Matrix Mimetics

United States Serial NO.: 13/994,179
Filed Date: December 14, 2011
Title: Chimeric Fibronectin Matrix Mimetics and Uses Thereof
Invented by: Denise Hocking, Daniel Roy
Controlling Collagen Microstructure with Ultrasound

United States Serial NO.:
Filed Date: March 14, 2013
Title: Controlling Collagen Microstructure with Ultrasound
Invented by: Diane Dalecki, Denise Hocking, Kelley Garvin

Recent Journal Articles

Showing the 5 most recent journal articles. 37 available »

2014 Jun
Mercado KP, Helguera M, Hocking DC, Dalecki D. "Estimating cell concentration in three-dimensional engineered tissues using high frequency quantitative ultrasound." Annals of biomedical engineering.. 2014 Jun; 42(6):1292-304. Epub 2014 Mar 14.
2014 Feb
Gildner CD, Roy DC, Farrar CS, Hocking DC. "Opposing effects of collagen I and vitronectin on fibronectin fibril structure and function." Matrix biology : journal of the International Society for Matrix Biology.. 2014 Feb; 34:33-45. Epub 2014 Feb 06.
2013 Nov
Roy DC, Mooney NA, Raeman CH, Dalecki D, Hocking DC. "Fibronectin matrix mimetics promote full-thickness wound repair in diabetic mice." Tissue engineering. Part A. 2013 Nov; 19(21-22):2517-26. Epub 2013 Aug 12.
2013 Aug
Garvin KA, Dalecki D, Yousefhussien M, Helguera M, Hocking DC. "Spatial patterning of endothelial cells and vascular network formation using ultrasound standing wave fields." The Journal of the Acoustical Society of America.. 2013 Aug; 134(2):1483-90.
2013 Aug
Garvin KA, Vanderburgh J, Hocking DC, Dalecki D. "Controlling collagen fiber microstructure in three-dimensional hydrogels using ultrasound." The Journal of the Acoustical Society of America.. 2013 Aug; 134(2):1491-502.

Current Appointments

Associate Professor - Department of Pharmacology and Physiology (SMD) - Primary
Associate Professor - Department of Biomedical Engineering (SMD)

Education

PhD | Physiology | Albany Medical College1992
MS | Physiology | Albany Medical College1990
BS | Medical Technology | Hartwick College1983

Post-Doctoral Training & Residency

Postdoctoral Research Fellow; Albany Medical College, Albany, NY. Topic: "Mechanisms of extracellular matrix fibronectin deposition". Advisor: Paula J. McKeown-Longo, Ph.D. 1996