Nancy Wang, Ph.D.

Nancy Wang, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 608
Rochester, NY 14642

Office: (585) 275-6597
Fax: (585) 273-3360

Research Bio

Microcell-mediated transfer of chromosome 11 into ovarian carcinoma cell line, SKOV-3 results in suppression of tumorigenicity in scid/scid mice providing evidence from functional aspect that chromosome 11 carries ovarian tumor suppressor gene(s).



To identify the differentially expressed transcripts associated with suppression of tumorigenicity, cDNA from the tumorigenic clones were subtracted from the non-tumorigenic clones which were then either cloned, sequenced and searched in GenBank, or analyzed by Gene Discovery Array Screening (GDAS). A cDNA transcript corresponding to the gene Thy-1 located (11q23-24) was found to be exclusively expressed in the non-tumorigenic clones. Transfection of Thy-1-antisence to the non-tumorigenic clone, 11(H)9-8, abolished the Thy-1 expression and restored tumorigenicity in scid/scid mice. We therefore, hypothesize that Thy-1 is a putative ovarian tumor suppressor gene (TSG).

To test this hypothesis, the following studies are ongoing: 1) To verify the tumor suppressing nature of Thy-1 and its specificity in fresh/frozen ovarian tumors by Northern blot, immunocytochemistry, and flow cytometry followed by statistical correlation study; 2) To define the effect of Thy-1 on in vivo growth and tumorigenicity by comparative xenographic study in scid/scid between 11(C)9-8-Thy-1-antisense and 11(C)9-8-null, as well as SKOV-3-Thy-1 and SKOV-3-null; 3) To identify the downstream genes associated with Thy-1 by comparative macroarray and microarray analysis combined with suppression subtractive hybridization on the DNA population between SKOV-3-Thy-1 and SKOV-3-null, as well as 11(C)9-8-Thy-1-antisense and 11(C)9-8-null; 4) To identify the sequential signaling pathway of Thy-1 using the in vitro doxycyclin (dox) Thy-1 inducible system established in our laboratory. cDNA obtained from SKOV-3-Thy-1-inducible cells at various time post dox exposure will be analyzed and compared between adjacent time points by both macroarray and dual color microarray.

The accomplishment of this study cannot only verify the OVC tumor suppressing nature of Thy-1, but also let us understand the function of this important cell surface marker.

Awards & Honors (Local)

Editorial Board | Cancer, Genetics and Cytogenetics 2009
President, Association of Chinese Geneticists in America 2000 - 2001
America's Outstanding Women of the 80's-America's Who's Who 1989
Tenured Associate Professorship, Dept of Biochemistry | Tulane University 1986
Charles E. Culpepper Fellowship | Tulane University 1985 - 1986
Appointed to mediate the Cancer and Developmental Genetics Session | Annual meeting for the American Socieity of Human Genetics | Toronto, Canada 1984

Recent Journal Articles

Showing the 5 most recent journal articles. 73 available »

2010
Chen YC,Tsai Y, Nowak I, Wang N, Hyrien O, Wilkins R, Ferrarotto C. Sun H, Dertinger SD. "Validating high-throughput micronucleus analysis of peripheral reticulocytes for radiation biodosimetry: Benchmark against dicentric and CBMN assays in a mouse model". Health Phys.. 2010; 98(2): 218-2010. Link
2009
Lung HL, Cheung AK, Cheng Y, Kwong FM, Lo PH, Law EWL, Chua D, Zabarovsky ER, Wang N, Tsao SW, Stanbridge EJ, Lung ML. "Functional characterization of THY-1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma." Int J Cancer. 2009; . Link
2007 Apr 15
Szych CM, Liesveld JL, Iqbal MA, Li L, Siebert S, Asmus C, O'Malley J, Lee A, Wang N. "Isodicentric Philadelphia chromosomes in imatinib mesylate (Gleevec)-resistant patients." Cancer genetics and cytogenetics. 2007 Apr 15; 174(2):132-7.
2007
Lichtman, M; Shafer, J; Felgar, R and Wang, N. www.accessmedicine.com/clinical.aspx. "Atlas of Hematological Diseases". McGraw-Hill Company. 2007; . Link
2005 Sep 29
Lung HL, Bangarusamy DK, Xie D, Cheung AK, Cheng Y, Kumaran MK, Miller L, Liu ET, Guan XY, Sham JS, Fang Y, Li L, Wang N, Protopopov AI, Zabarovsky ER, Tsao SW, Stanbridge EJ, Lung ML. "THY1 is a candidate tumour suppressor gene with decreased expression in metastatic nasopharyngeal carcinoma." Oncogene. 2005 Sep 29; 24(43):6525-32.

Current Appointments

Professor - Department of Pathology and Laboratory Medicine (SMD) - Primary

Education

PhD | Genetics | Univ Minnesota-Twin City Cmp1978
MS | Genetics | Univ Minnesota-Twin City Cmp1968
BS | Botany | Taiwan - Non-Medical School1966