Lisa A. DeLouise, Ph.D., M.P.D.

Lisa A. DeLouise, Ph.D., M.P.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 697
Rochester, NY 14642

Office: (585) 275-1810
Lab: (585) 273-4689
Fax: (585) 273-1346
Administrative: (585) 275-3871

Lab Information

We are bionanomaterials group that investigates novel biomedical devices for diagnostic and therapeutic applications.

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Research Bio

Research Interest:
The overall research area in the laboratory is the creation of Smart Bandage Biomaterial Engineering and Skin Disease. This laboratory investigates the fundamental optical, morphological, and surface chemical properties of bioengineered nanoporous silicon (PSi) in developing a platform of Smart Bandage technologies targeting biosensors for point of care diagnosis of cutaneous disease, transdermal drug delivery, and tissue engineering for wound healing. PSi is fabricated from single crystal silicon wafers using an electrochemical etch process. The pore diameter, porosity (surface area and internal void volume), bioerosion, and interferometric optical properties can be tailored over a wide range to suit the application.

Ongoing projects involve developing a refractive index sensitive biosensor for detection of Candida ablicans for which we've developed methods to site direct the immobilization of phage display scFv antibody receptors. Tests are underway to evaluate detection sensitivity relative to nonspecifically immobilized whole IgG aCandida antibody receptor employing commercial antigen. Fundamental insights into the factors (pore size, steric crowding, operating frequency, device architecture, blocking agent, operation protocol, etc.) that impact detection sensitivity are being explored. Biosensor tests are typically conducted on devices attached to the silicon wafer. Recently, we developed methods to detach the sensor and mount it in a polymer support. We are conducting studies to characterize the diffusion characteristics of small molecules though hydrogel films (40 micron) cast over porous silicon sensor (4 um) by monitoring changes in the optical response that result when molecules diffuse in or out of the porous silicon layer. This work will enable us to develop models for designing transdermal drug delivery systems where the optical response can be measured, while applied to the skin, to monitor the time released delivery of therapeutics concentrated within the porous reservoir. We are interested in developing a smart bandage to deliver antifungal locally to the nail matrix where nail progenitor cells live. We are also investing the morphology and phenotype dermal human fibroblast and immortalized keratinocytes (HaCaT) cells cultured on chemically modified PSi and flat silicon wafer surfaces. The goal is systematically engineer the surface chemistry, energy and topography of biomaterial scaffolds to mimic the fetal reepithelialization process. Our hypothesis is that by controlling differential cell proliferation (keratinocyte vs. fibroblast) and cell phenotype, such as integrin and metalloproteinase expression profiles or the magnitude of actin fiber extensions, novel therapies will result that can accelerate the healing of chronic wounds (ulcers) and burns with reduced scarring. A unique aspect of this research program is the melding of cross-disciplinary skills in surface science, physical chemistry, microfluidic device engineering, optics, and biological and medical sciences. This interdisciplinary approach provides a firm basis for the investigation and fabrication of new biomedical devices, while enabling a broader perspective on quantifying biological efficacy and establishing clinical utility.

Patents

Integrated Microfluidic Bubble Bioreactor for Cell Capture

United States Serial NO.: 12/139,797
Filed Date: June 16, 2008
Title: Microfluidic Device and Method of Manufacturing the Microfluidic Device
Invented by: Lisa DeLouise, Ut-Binh Thi Giang, Mike King
Integrated Microfluidic Bubble Bioreactor for Cell Capture

United States Serial NO.: 13/304,843
Filed Date: November 28, 2011
Title: Microfluidic Device and Method of Manufacturing the Microfluidic Device
Invented by: Lisa DeLouise, Ut-Binh Thi Giang, Mike King
Integrated Microfluidic Bubble Bioreactor for Cell Capture

United States Serial NO.: 13/469,184
Filed Date: May 11, 2012
Title: Microfluidic Device and Method of Manufacturing the Microfluidic Device
Invented by: Lisa DeLouise, Ut-Binh Thi Giang, Mike King
Target Antigen Responsive Polymer (TRAP) with Integrated Porous Silicon Optical Sensor

United States Serial NO.: 13/035,887
Filed Date: February 25, 2011
Title: Hybrid Target Analyte Responsive Hydrogel (TRAP-gel)- Porous Silicon Sensor
Invented by: Lisa Bonanno, Lisa DeLouise
Novel In vitro Assays for Enriching and Determining the Clonogenic Potential of Tumor Initiating Cells

United States Serial NO.: 13/544,555
Filed Date: July 9, 2012
Title: Method of Enriching Stem and/or Progenitor Cells
Invented by: Lisa DeLouise, Siddarth Chandrasekaran

Recent Journal Articles

Showing the 5 most recent journal articles. 76 available »

2014 Feb
Giang UB, Jones MC, Kaule MJ, Virgile CR, Pu Q, Delouise LA. "Quantitative analysis of spherical microbubble cavity array formation in thermally cured polydimethylsiloxane for use in cell sorting applications." Biomedical microdevices.. 2014 Feb; 16(1):55-67.
2014
Jatana S, DeLouise LA. "Understanding engineered nanomaterial skin interactions and the modulatory effects of ultraviolet radiation skin exposure." Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology.. 2014 6(1):61-79. Epub 2013 Oct 03.
2013 Nov
Mortensen LJ, Ravichandran S, Delouise LA. "The impact of UVB exposure and differentiation state of primary keratinocytes on their interaction with quantum dots." Nanotoxicology. 2013 Nov; 7(7):1244-54. Epub 2012 Oct 25.
2013 Jun
Jones MC, Kobie JJ, Delouise LA. "Characterization of cell seeding and specific capture of B cells in microbubble well arrays." Biomedical microdevices.. 2013 Jun; 15(3):453-63.
2013 Mar
Zheng H, Mortensen LJ, DeLouise LA. "Thiol antioxidant-functionalized CdSe/ZnS quantum dots: synthesis, characterization, cytotoxicity." Journal of biomedical nanotechnology.. 2013 Mar; 9(3):382-92.

Current Appointments

Associate Professor - Department of Dermatology (SMD) - Primary
Associate Professor - Department of Biomedical Engineering (SMD)
Associate Professor - Department of Electrical & Computer Engineering (RC)

Education

MPD | Product Development | Rochester Inst Technology2001
PhD | Physical Chemistry | Penn State University1984
BS | Chemistry | Providence College1979