Paul J. Kammermeier, Ph.D.

Paul J. Kammermeier, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 711
Rochester, NY 14642

Office: (585) 275-5606
Lab: (585) 275-1498
Fax: (585) 273-2652

Research Bio

My research interests center around G protein-coupled receptor function, particularly metabotropic glutamate receptors (mGluRs), and ion channel modulation in neurons. Naturally, this work has led me toward an interest in heterotrimeric G proteins, G protein-coupled receptors and factors that modulate G protein signaling, such as the Regulators of G protein Signaling (RGS) proteins (see Kammermeier and Ikeda, 1999, Neuron 22:819). I have also become interested in the Homer family of scaffolding proteins, which I have shown to be important in the function and sub-cellular localization of metabotropic glutamate receptors (Kammermeier et al., 2000, Journal of Neuroscience 220:7238; Kammermeier, 2005, BMC Neuroscience 7:1). In the long term, my goal is to understand the mechanisms of regulation, localization and signaling that impart specificity to the various mGluRs, and how regulation of mGluR signaling alters synaptic strength in the nervous system.
In my laboratory, two basic experimental approaches are used. The first utilizes primary cultures of isolated SCG neurons for electrophysiological, molecular biological, immunofluorescence and RT-PCR experiments. The main technique used in this system is to examine the well-characterized ion channel modulatory pathways using patch-clamp electro-physiology as an assay for G protein coupled receptor activity. The neurons can be made to express various heterologous proteins (including mGluRs) via intranuclear injection of cDNA. I have recently used this system to examine mGluR-G protein coupling specificity, mGluR desensitization and the regulation of mGluR signaling and trafficking by various associated proteins.



The second approach is to use native neuronal cells. Currently, we are working with both primary isolated ON Bipolar cells from the rat retina and with rat hippocampal neurons to examine G protein coupling of mGluR6 and the effects of Homer protein regulation of mGluR5 at synapses, respectively. These systems allow us to ask questions about the physiological relevance in parallel with the more tractable, highly amenable SCG system described above.

Finally, we are beginning to incorporate other fluorescence-based techniques such as total internal reflection fluorescence (TIRF) microscopy (Kammermeier, 2005, BMC Neuroscience 7:1) to examine protein distribution in or very near the cell membrane.

Recent Journal Articles

Showing the 5 most recent journal articles. 27 available »

2013 Mar
Beqollari D, Kammermeier PJ. "The interaction between mGluR1 and the calcium channel Cav?.? preserves coupling in the presence of long Homer proteins." Neuropharmacology. 2013 Mar; 66:302-10. Epub 2012 May 30.
2012 Sep
Kammermeier PJ. "Functional and pharmacological characteristics of metabotropic glutamate receptors 2/4 heterodimers." Molecular pharmacology.. 2012 Sep; 82(3):438-47. Epub 2012 May 31.
2012 Jun
Hu JH, Yang L, Kammermeier PJ, Moore CG, Brakeman PR, Tu J, Yu S, Petralia RS, Li Z, Zhang PW, Park JM, Dong X, Xiao B, Worley PF. "Preso1 dynamically regulates group I metabotropic glutamate receptors." Nature neuroscience.. 2012 Jun; 15(6):836-44.
2012
Kammermeier PJ. "The orthosteric agonist 2-chloro-5-hydroxyphenylglycine activates mGluR5 and mGluR1 with similar efficacy and potency." BMC pharmacology. 2012 12:6. Epub 2012 May 29.
2010 Jul
Beqollari D, Kammermeier PJ. "Venus fly trap domain of mGluR1 functions as a dominant negative against group I mGluR signaling." Journal of neurophysiology.. 2010 Jul; 104(1):439-48. Epub 2010 May 12.

Current Appointments

Associate Professor - Department of Pharmacology and Physiology (SMD) - Primary

Education

PhD | Neuroscience | Case Western Reserve Univ1997
BS | Neuroscience | Univ of Pittsburgh1990

Post-Doctoral Training & Residency

Research Fellow: National Institutes of Health (NIAAA), Rockville, MD. Advisor: Stephen R. Ikeda, M.D., Ph.D. 2002
Post-Doctoral Associate: Guthrie Research Institute, Sayre, PA. Advisor: Stephen R. Ikeda, M.D., Ph.D. 2002
Post-Doctoral Associate: University of Florida, Department of Physiology, Gainesville, FL. Principal Advisor: Philip Posner, Ph.D. 1998