Mark Y. Sangster, Ph.D.

Mark Y. Sangster, Ph.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 609
Rochester, NY 14642

Research Bio

Overview



Virus-specific antibodies play a key role in providing a protective barrier to infection and in facilitating viral clearance once an infection is established. Antibody-producing cells or plasma cells are generated from B cells that divide and differentiate following recognition of specific antigen. A critical requirement for optimal antibody responses is the cognate help delivered to activated B cells by CD4 T cells. This help is important for directing antibody isotype switching in B cells, the process by which B cells switch from expressing IgM to expressing alternative isotypes (such as IgG1, IgG2a, and IgA in the mouse) with different functional characteristics. In addition, cognate T cell help is critical if activated B cells are to participate in germinal center reactions, where affinity maturation of the antibody response takes place and the cellular elements of B cell memory are generated. These elements include long-lived plasma cells, which maintain high levels of protective antibodies, and a population of memory B cells that will respond with rapid antibody production on re-exposure to cognate antigen. This brief overview grossly simplifies a remarkable and complex process that is regulated at many levels in ways that modulate the kinetics, magnitude, and quality of the acute B cell response, as well as characteristics of dispersed plasma cell and memory B cell populations. In general terms, our research is aimed at understanding the characteristics of optimally effective B cell responses and applying this knowledge towards the improvement of vaccination regimens.

Specific Research Interests

Our specific research focus is the B cell response to viruses that infect the respiratory tract (with an emphasis on influenza virus) and to vaccination regimens designed to generate B cell-mediated protection against these viruses. Particular research interests include: (i) regulation of the acute B cell response to infection and vaccination and the differentiation pathways that generate B cell memory and virus-neutralizing antibodies, (ii) aspects of B cell memory (plasma cells, memory B cells) in the respiratory tract, including mechanisms of localization, role in protection, and relationship to form of immunization, and (iii) regulation of IgA production and the establishment of antibody-mediated protection at mucosal surfaces.

Awards & Honors (Local)

Athelstan and Amy Saw Medical Research Award | University of Western Australia 1991
Australian Commonwealth Postgraduate Award for Dissertation Research 1985
Swan Brewery Prize for Undergraduate Honors Thesis | University of Western Australia 1984

Recent Journal Articles

Showing the 5 most recent journal articles. 49 available »

2014 Nov 28
Babu TM, Levine M, Fitzgerald T, Luke C, Sangster MY, Jin H, Topham D, Katz J, Treanor J, Subbarao K. "Live attenuated H7N7 influenza vaccine primes for a vigorous antibody response to inactivated H7N7 influenza vaccine." Vaccine. 2014 Nov 28; 32(50):6798-6804. Epub 2014 Oct 16.
2014 Nov 22
Sundararajan A, Sangster MY, Frey S, Atmar RL, Chen WH, Ferreira J, Bargatze R, Mendelman PM, Treanor JJ, Topham DJ. "Robust mucosal-homing antibody-secreting B cell responses induced by intramuscular administration of adjuvanted bivalent human norovirus-like particle vaccine." Vaccine. 2014 Nov 22; Epub 2014 Nov 22.
2014 Jan
Alam S, Knowlden ZA, Sangster MY, Sant AJ. "CD4 T cell help is limiting and selective during the primary B cell response to influenza virus infection." Journal of virology. 2014 Jan; 88(1):314-24. Epub 2013 Oct 23.
2014
Miao H, Sangster MY, Livingstone AM, Hilchey SP, Zhang L, Topham DJ, Mosmann TR, Holden-Wiltse J, Perelson AS, Wu H, Zand MS. "Modeling the dynamics and migratory pathways of virus-specific antibody-secreting cell populations in primary influenza infection." PloS one. 2014 9(8):e104781. Epub 2014 Aug 29.
2014
Berry CM, Penhale WJ, Sangster MY. "Passive broad-spectrum influenza immunoprophylaxis." Influenza research and treatment. 2014 2014:267594. Epub 2014 Sep 22.

Current Appointments

Research Associate Professor - Department of Microbiology and Immunology, Center for Vaccine Biology and Immunology (SMD) - Primary

Education

PhD | Genetics | Australia-U Western Australia - Perth1991
BS | Genetics | Australia-U Western Australia - Perth1984