Nikon A1R HD with TIRF

Capabilities:

• Laser Scanning Confocal Microscopy (Standard and Resonant scanners available)
• Total Internal Reflection Fluorescence Microscopy (TIRF)
• Epi-fluorescent Microscopy
• Single Molecule Imaging
• Differential Interference Contrast (DIC)

Summary of Instrument Features:

Microscope: Inverted Nikon Ti2-E

Software: NIS-Elements C with JOBS Acquisition Module

Objectives:

• Standard:
  60x/1.49 - Oil - Apochromat TIRF
  40x/1.15 - Water – Apochromat Lambda LWD
  20x/0.75 - Air – Plan Apochromat VC
  10x/0.40 - Air – Plan Apochromat Lambda
  4x/0.2 - Air – Plan Apochromat Lambda

Laser lines:

• Excitation:
  405nm diode
  445nm diode
  488nm diode
  515nm diode
  561nm diode
  640nm diode

Detectors: 2 GaAsP + 2 High-sensitivity PMT

Stage:  Motorized stage

• 200um Piezo Z stage
• Perfect Focus z-stabilization
• Multi and Plate holders available

Environmental Chamber: OKO Labs

• Heating control
• 5% CO2
• Humidity Control