Zeiss Palm MicroBeam Laser Capture Microdissection

Capabilities:

• Selectively capture cells or tissue segments from larger cultures or tissue sections for further analysis
• Epi-fluorescent Microscopy
• Color Histology/Microscopy

Summary of Instrument Features:

Microscope: Upright Zeiss Axio Observer Z1

Software: Zeiss AxioVision

Objectives:

• Standard:
  63x/1.4  - Oil – Plan Apochromat
  60x/0.75 - Air – Plan NeoFluar  
  40x/0.6 - Air – Plan NeoFluar
  20x/0.4  - Air – Plan NeoFluar
  10x/0.05 - Air – Fluar

Light source:

• Excitation:
  Lumen Dynamics X-Cite Series 120Q Lamp
• Capture:
  Pulsed UV Laser

Cameras:

• Zeiss CC1 Color Camera
• ​​Zeiss mRM BW Camera

Stage:  Motorized stage

• 3-Slide sample holder
• 2-Cap holder for sample collection

Protocols:

• RNA extraction from FFPE sections (Zeiss)
• PALM protocols-DNA handling (Zeiss)
• RNA extraction from frozen sections (Zeiss)
• LCM protocols-RNA handling (Zeiss)
• Laser Microdissection:  A key technology for Microproteomics (Zeiss)
• Tissue scrape protocol for verifying RNA Quality (Arcturus)