UR Faculty: Ingrid H. Sarelius, Ph.D.

Ph.D. (1978)
University of Auckland, New Zealand

Ingrid H. Sarelius, Ph.D.
Professor of Pharmacology and Physiology and of Biomedical Engineering

Primary Appointment:
Pharmacology and Physiology

GEBS Cluster Affiliation:
CMM-Cellular and Molecular Basis of Medicine

Program Affiliations:
BME-Biomedical Engineering Program

Research:
Vascular cell communication and signaling

Sarelius's Lab Page

Contact Information:
E-mail: Ingrid_Sarelius@urmc.rochester.edu

Contact Information:
University of Rochester
School of Medicine and Dentistry
601 Elmwood Ave, Box 711
Rochester, New York 14642

Medical Center, Room 4-8531
Phone: (585) 275-7729
Fax: (585) 273-2652

Research Overview

We are interested in the pathways by which the cells of the blood vessel wall are able to communicate with each other and respond to both local and remote signals, such as those from exercising muscle, from changes in blood flow, or from other blood cells. Our focus is on three topics. (1) Local metabolic stimuli produced by contraction of 4-5 skeletal muscle fibers initiate signals in capillaries and small arterioles that spread rapidly to cause dilation at remote upstream sites. This dilatory signal is transmitted along the blood vessel wall via a pathway that probably involves both gap junctions and local paracrine events. Our interest is in defining these signaling pathways and in identifying the initiating signal from skeletal muscle. (2) Endothelial cells grown in microvascular sized tubes respond to flow differently from cells growing in environments relevant to large blood vessels; they have different cytoskeletal organization when growing on curved versus flat surfaces, and cytoskeletal responses to flow are different on the two surface shapes. We are exploring the mechanisms underlying these differences. (3) The normal regulation of leukocyte-endothelial interactions in microvessels in vivo is not as expected from studies on isolated cells, in large part because the hemodynamic conditions are different, which affects both leukocyte behavior and adhesion molecule expression. We are using confocal microscopy and other imaging techniques to determine both wall shear stresses and adhesion molecule distributions in small venules in situ, and to investigate the signaling mechanisms underlying leukocyte-endothelial interactions.

Recent Publications

Duza, T., and Sarelius, I.H. (2004) Increase in endothelial cell Ca(2+) in response to mouse cremaster muscle contraction. J. Physiol. 555 (Pt 2):459-469.

Duza, T., and Sarelius, I.H. (2004) Localized transient increases in endothelial cell Ca2+ in arterioles in situ: implications for coordination of vascular function. Am. J. Physiol. 286:H2322-H2331.

Kim, M.B., and Sarelius, I.H. (2004) Regulation of leukocyte recruitment by local wall shear rate and leukocyte delivery. Microcirculation 11:55-67.

Kim, M.B., and Sarelius, I.H. (2004) Role of shear forces and adhesion molecule distribution on P-selectin-mediated leukocyte rolling in postcapillary venules. Am. J. Physiol. 287:H2705-H2711.

King, M.R., Bansal, D., Kim, M.B., and Sarelius, I.H. (2004) The effect of hematocrit and leukocyte adherence on flow direction in the microcirculation. Ann. Biomed. Eng. 32:803-814.

Murrant, C.L., Duza, T., Kim, M.B., Cohen, K.D., and Sarelius, I.H. (2004) Arteriolar dilations induced by contraction of hamster cremaster muscle are dependent on changes in endothelial cell calcium. Acta Physiol. Scand. 180:231-238.

Wojciechowski, J.C., and Sarelius, I.H. (2005) Preferential binding of leukocytes to the endothelial junction region in venules in situ. Microcirculation 12:349-359.

Sarelius, I.H., Kuebel, J.M., Wang, J., and Huxley, V.H. (2006) Macromolecule permeability of in situ and excised rodent skeletal muscle arterioles and venules. Am. J. Physiol. 290:H474-H480.

PubMed Publication List

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and provides complete abstracts of all 'sarelius ih' publications,
as well as links to the full text of many articles (at journal homepages).

Back to Pharmacology and Physiology

GEBS Clusters:

CMM