Myoblast Cell Cultures

The following describes the methods used in the Fields Center for initiation of myoblast cell cultures from FSHD (FSH dystrophy) and conrol individuals.

 

Background: Myoblast cell cultures are established from samples of muscle obtained either through an needle or open biopsy sampling procedure. Typically a muscle sample of between 60-100mg is adequate for establishing a primary myoblast culture.

A. Preparing Primary Myoblast Cultures (updated 4/15/2014) (Download PDF file)

B. MRC-5 Conditioning Media (updated 4/15/2014) (Download PDF file)

C. Desmin Staining (Download PDF file)

D. Reference (Download PDF file)

 

For more information, please contact the Fields Center FieldsCenter@urmc.rochester.edu