Analysis

Analytical Flow Cytometry - FACSCanto II

The Flow Facility is equipped with a 3-laser, 8-paramenter Becton-Dickinson FacsCantoII for analytic flow cytometry. Because of the unique optical bench, the system has a great deal of flexibility in the exact fluorochromes that can be detected.

 

Analytical Flow Cytometry - LSR II 18-Color

 

Analytical Flow Cytometry - LSR II 12-Color

 

Analytical Flow Cytometry - LSR II 8-Color

 

Note: Additionally, all LSR II have the capability to use a high-throughput sampling (HTS) device.

 

Analytical Flow Cytometry - Accuri C6

 

 

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.



CVRI Flow Core Instrument

Accuri C6

Fee Structure:

Time booked (via online calendar) or time used (whichever is longer)

Billed at $25/hour (billed to the minute)
+$20 for operater assisted runs

 

Please note that the Accuri C6 is for use by training only! Contact Tim to get trained.

C6 Configuration: 

The C6 is arranged in such a way that four detectors all simultaneously measure light scatter. The detector ranges are found in the charts below.

488 Laser  || 640 Laser

BLUE 488 LASER (10mW): Measures two scatter parameters: FSC and SSC. Also measures the following stains:

Detector

Band Pass

Fluor's

FL1

530/30

FITC, GFP

FL2

585/40

PE, PI

FL3

670 LP

PerCP-Cy5.5, PE-Cy5, PE-Cy7

RED 640 LASER (10mW):

Detector

Band Pass

Fluor's

FL4

675/25

APC

 

 

Location of URMC Flow Core Instruments

  • Dr Bunsen - 3-4151
  • Rowlf - 3-4151
  • Pepe - 3-4151
  • Fozzie Bear - 3-4141
  • Animal - 3-4141
  • Kermit - 3-4141
  • Gonzo - 3-4141
  • Accuri C6 - CVRI C206