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URMC  » Flow Cytometry Core » Instruments

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Analysis

Analytical Flow Cytometry - FACSCalibur

The Flow Facility has two 4-color analog FACSCaliburs, one located in 3-4100 area, and one on the 3rd floor of the MRB.

• Fee Structure 
• Guidelines  
• Configuration

Analytical Flow Cytometry - FACSCanto II

The Flow Facility is equipped with a 3-laser, 14-paramenter Becton-Dickinson LSR-II for analytic flow cytometry. Because of the unique optical bench, the system has a great deal of flexibility in the exact fluorochromes that can be detected.

• Fee Structure 
• Guidelines  
• Configuration- 405 Laser 488 Laser 633 Laser 
• Suggested Voltages (08/31/09) - 405 488 633

 

Analytical Flow Cytometry - LSR II 18-Color

This is a state-of-the-art digital flow cytometer is capable of detecting 18 fluorochromes simultaneously. This system uses a 532 nm laser for excitation of PE and PE-tandems, improving their signal strenght. The system is also capable of detecting Q-Dot conjugated molecules.

• Fee Structure 
• Guidelines  
• Configuration- 407 Laser 488 Laser 532 Laser 633 Laser   
• Suggested Voltages (09/08/09) - 405 488 532 633

 

Analytical Flow Cytometry - LSR II 12-Color

• Fee Structure 
• Guidelines  
• Configuration- 405 Laser 488 Laser 633 Laser 
• Suggested Voltages (08/31/09) - 405 488 633

 

Analytical Flow Cytometry - LSR II 12-Color

• Fee Structure 
• Guidelines  
• Configuration- 405 Laser 488 Laser 633 Laser
• Suggested Voltages (08/31/09) - 405 488 633

 

Note: Additionally, all three LSR II have the capability to use a high-throughput sampling (HTS) device.

 

Analutical Flow Cytometry - Accuri C6

• Fee Structure 
• Guidelines  
• Configuration - 488 Laser 640 Laser

 

Contact Tim: Email or Messenger (bushnt) or 273-5535

 

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

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Flow Core Instruments

FACSCalibur

Fee Structure:

$35/hour billed to the minute.

Calibur LayoutLasers: 488 nm Blue (15 mW) and 633 nm Red (20 mW)
4PMTs: Typically FITC/GFP, PE, PI and APC or FITC/GFP, PE, PE-Cy5 and APC

COMING SOON: USER GUIDES and TROUBLESHOOTING Tips

FACSCanto II

Fee Structure: Time booked (via online calendar) or time used (whichever is longer) Billed at $35/hour (billed to the minute) +$20/hr operated assisted runs

FACSCanto II Configuration:  488 Laser  || 633 Laser  || 405 Laser BLUE 488 LASER: Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max BLUE A 735 780/60 PE-Cy7 600 185 243115 BLUE B 655 670LP PerCP-Cy5.5, PE-Cy5.5, PE-Cy5, 7AAD, PerCP 550 185 238971 BLUE C - 610         BLUE D 556 585/42 PE, DSRed, PI 500 93 240105 BLUE E 502 530/30 FITC, GFP, YFP, AlexaFluor 430, AlexaFluor 488, TO 550 40 238670 BLUE F - 488/10 SSC       BLUE G - -         BLUE H --- -

RED 633 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max RED A 735 780/60 APC-Cy7 500 158 238155 RED B 685 -         RED C --- 660/20 APC, Alexa 647, ToPro3 500 26 238642

VIOLET 405 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max VIOLET A 502 550/50 Pacific Orange 500 84 233107 VIOLET B 450/50 Pacific Blue, DAPI, Hoechst 33258, Vibrant Violet 400 73 238004 VIOLET C - -

 

18 Color LSR II

Fee Structure: Time booked (via online calendar) or time used (whichever is longer) Billed at $50/hour (billed to the minute) +$20 for operater assisted runs HTS Compatable

LSR-II Configuration:  488 Laser  || 633 Laser  || 407 Laser || 532 Laser BLUE 488 LASER: Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max BLUE A 685 710/50 PerCP-Cy5.5, PerCP 600 25 234981 BLUE B 505 515/20 FITC, CFSE, GFP, Alexa 488, R123 600 47 220756 BLUE C   488/10 SSC       FSC

RED 633 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max RED A 740 780/60 APC-Cy7, APC-Alexa 750 600 188 242038 RED B 685 710/50 APC-Cy5.5, Alexa 700, Alexa 680 600 396 248477 RED C 660/20 APC, Alexa 647 500 92 227831

VIOLET 407 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max VIOLET A 740 780/60 Qdot 800 750 104 229644 VIOLET B 670 705/70 Qdot 705 650 16 232948 VIOLET C 630 660/40 Qdot 655 500 9 233760 VIOLET D 595 605/40 Qdot 605 650 194 123591 VIOLET E 570 585/42 Qdot 585 500 80 222149 VIOLET F 557 560/40 Qdot 565 450 12 236637 VIOLET G 535 550/40 Pacific Orange, Alexa 430, Qdot 545 450 18 234445 VIOLET H   450/50 Pacific Blue, Alexa 405, Cascade Blue 450 70 229216

GREEN 532 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max GREEN A 740 780/40 PE-Cy7, PE-Alexa 750 550 237 255655 GREEN B 690 710/50 PE-Cy5.5 450 18 255036 GREEN C 640 660/40 7AAD, PE-Cy5 400 17 240976 GREEN D 600 610/20 PE-Alexa 610, PE-Texas Red 400 24 170867 GREEN E 575/25 PE, Alexa 532 600 71 228112

 

12 Color LSR II (RHIC)

Fee Structure: Time booked (via online calendar) or time used (whichever is longer) Billed at $42/hour (billed to the minute) +$20 for operater assisted runs   HTS Compatable LSR-II (2-9905)

LSR-II Configuration:  488 Laser  || 633 Laser  || 405 Laser BLUE 488 LASER: Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max BLUE A 735 780/60 PE-Cy7, PE-Alexa750 550 66 240432 BLUE B 685 695/40 PerCP-Cy5.5, PE-Cy5.5, PerCP, 7AAD, PE-Alexa700, PE-Alexa680 650 19 223475 BLUE C 665 670/14 PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++ 550 14 219614 BLUE D 600 610/20 PI, PE-Texas Red, PE-Alexa610, PE-Alexa594 500 121 238735 BLUE E 550 576/26 PE 600 48 220782 BLUE F 505 530/30 FITC, Alexa488, YFP, GFP, CFSE 500 44 235517 BLUE G   488/10 SSC

RED 633 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max RED A 735 780/60 APC-Cy7, APC-Alexa750, APC-H7 500 160 246145 RED B 685 720/40 APC-Cy5.5, AlexaFluor 700, AlexaFluor 680 450 12 242881 RED C 660/20 APC, Alexa 647 400 5 239369

VIOLET 405 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max VIOLET A 595 610/20 Qdot 605 550     VIOLET B 505 525/50 AlexaFluor 430, CFP, Fura-Hich_Ca++, Pacific Orange 500 89 235747 VIOLET C 450/50 AlexaFluor 405, DAPI, Marina Blue, Pacific Blue, Sytox Blue 300 42 220220

 

12 Color LSR II (Flow Core)

Fee Structure: Time booked (via online calendar) or time used (whichever is longer) Billed at $42/hour (billed to the minute) +$20 for operater assisted runs   HTS Compatable LSRII - 3-4141

LSR-II Configuration:  488 Laser  || 633 Laser  || 405 Laser BLUE 488 LASER: Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max BLUE A 735 780/60 PE-Cy7, PE-Alexa750 550 33 262143 BLUE B 685 710/50 PerCP-Cy5.5, PE-Cy5.5, PerCP, 7AAD, PE-Alexa700, PE-Alexa680 550 68 262143 BLUE C 665 660/20 PE-Cy5, PE-Alexa647, 7AAD, Fura-Low_Ca++ 550 79 262143 BLUE D 600 610/20 630/30 PI, PE-Texas Red, PE-Alexa610, PE-Alexa594 550 89 262143 BLUE E 550 576/26 PE 400 8 243532 BLUE F 505 525/50 FITC, Alexa488, YFP, GFP, CFSE 450 70 239213 BLUE G   488/10 SSC

RED 633 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max RED A 735 780/60 APC-Cy7, APC-Alexa750, APC-H7 500 3 262143 RED B 685 710/50 APC-Cy5.5, AlexaFluor 700, AlexaFluor 680 500 60 262143 RED C 660/20 APC, Alexa 647 500 15 262143

VIOLET 405 LASER Detector LP Band Pass Fluor's Suggested Voltage Linear Range Min Max VIOLET A 595 610/20 Qdot 605 450 218 262143 VIOLET B 505 525/50 AlexaFluor 430, CFP, Fura-Hich_Ca++, Pacific Orange 450 27 262143 VIOLET C 450/50 AlexaFluor 405, DAPI, Marina Blue, Pacific Blue, Sytox Blue 350 34 262143

Accuri C6

Fee Structure: Time booked (via online calendar) or time used (whichever is longer) Billed at $20/hour (billed to the minute) +$20 for operater assisted runs   Please note that the Accuri C6 is for use by training only! Contact Tim to get trained.

C6 Configuration:  The C6 is arranged in such a way that four detectors all simultaneously measure light scatter. The detector ranges are found in the charts below. 488 Laser  || 640 Laser BLUE 488 LASER: Measures two scatter parameters: FSC and SSC. Also measures the following stains: Detector Band Pass Fluor's FL1 530/30 FITC, GFP FL2 585/40 PE, PI FL3 670 LP PerCP-Cy5.5, PE-Cy5, PE-Cy7

RED 640 LASER: Detector Band Pass Fluor's FL4 675/25 APC

 

 

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