Fozzie Bear Configuration

Suggested Voltage current as of July 24, 2014.

Detailed information about this instrument can be found in the baseline report here.

Reservations

Reservations can be made online by following this link.

You will need an approved USER account. Apply for one here.

 

LSR-II Configuration: 

488 Laser  || 633 Laser  || 407 Laser || 532 Laser

BLUE 488 LASER (100mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

BLUE A

685

710/50

PerCP-Cy5.5

550

BLUE B

505

515/20

FITC, CFSE, GFP, Alexa 488

550

BLUE C

  488/10 SSC  

FSC

 

RED 633 LASER (40mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

RED A

740

780/60

APC-Cy7, APC-Alexa 750

650

RED B

685 710/50 APC-Cy5.5, Alexa 700, Alexa 680

600

RED C

660/20

APC, Alexa 647

500

VIOLET 407 LASER (35mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

VIOLET A

740

780/60

Qdot 800, BV 786

800

VIOLET B

670

705/70

Qdot 705, BV 711

600

VIOLET C

630

660/40

Qdot 655, BV 650

500
VIOLET D 595 605/40 Qdot 605, BV 605 450
VIOLET E 570 585/42 Qdot 585 550
VIOLET F 557 560/40 Qdot 565 500
VIOLET G 535 550/40 Pacific Orange, BV 510, Qdot 545 500
VIOLET H   450/50 Pacific Blue, Alexa 405, BV 421 450

GREEN 532 LASER (120mW):

Detector

LP

Band Pass

Fluor's

Suggested Voltage

GREEN A

740

780/40

PE-Cy7, PE-Alexa 750

700

GREEN B

690

710/50

PE-Cy5.5

450

GREEN C

640 660/40 7AAD, PE-Cy5 500

GREEN D

600

610/20

PE-Alexa 610, PE-Texas Red

350

GREEN E

575/25

PE, Alexa 532, PI

550