Fozzie Bear Configuration
Suggested Voltage current as of 11 March 2013.
Detailed information about this instrument can be found in the baseline report here.
Reservations
Reservations can be made online by following this link.
You will need an approved USER account. Apply for one here.
LSR-II Configuration:
488 Laser || 633 Laser || 407 Laser || 532 Laser
BLUE 488 LASER:
Detector |
LP |
Band Pass |
Fluor's |
Suggested Voltage |
BLUE A |
685 |
710/50 |
PerCP-Cy5.5 |
550 |
BLUE B |
505 |
515/20 |
FITC, CFSE, GFP, Alexa 488, R123 |
550 |
BLUE C |
|
488/10 |
SSC |
|
FSC |
|
|
|
|
|
RED 633 LASER
Detector |
LP |
Band Pass |
Fluor's |
Suggested Voltage |
RED A |
740 |
780/60 |
APC-Cy7, APC-Alexa 750 |
600 |
RED B |
685 |
710/50 |
APC-Cy5.5, Alexa 700, Alexa 680 |
650 |
RED C |
|
660/20 |
APC, Alexa 647 |
550 |
|
VIOLET 407 LASER
Detector |
LP |
Band Pass |
Fluor's |
Suggested Voltage |
VIOLET A |
740 |
780/60 |
Qdot 800 |
700 |
VIOLET B |
670 |
705/70 |
Qdot 705 |
600 |
VIOLET C |
630 |
660/40 |
Qdot 655 |
450 |
| VIOLET D |
595 |
605/40 |
Qdot 605 |
450 |
| VIOLET E |
570 |
585/42 |
Qdot 585 |
550 |
| VIOLET F |
557 |
560/40 |
Qdot 565 |
450 |
| VIOLET G |
535 |
550/40 |
Pacific Orange, Alexa 430, Qdot 545 |
450 |
| VIOLET H |
|
450/50 |
Pacific Blue, Alexa 405, Cascade Blue |
450 |
|
GREEN 532 LASER
Detector |
LP |
Band Pass |
Fluor's |
Suggested Voltage |
GREEN A |
740 |
780/40 |
PE-Cy7, PE-Alexa 750 |
550 |
GREEN B |
690 |
710/50 |
PE-Cy5.5 |
400 |
GREEN C |
640 |
660/40 |
7AAD, PE-Cy5 |
350 |
GREEN D |
600 |
610/20 |
PE-Alexa 610, PE-Texas Red |
350 |
GREEN E |
|
575/25 |
PE, Alexa 532 |
500 |
|
