Imaging

Originally sponsored by the James P. Wilmont Cancer Center, the acquisition and upgrading of the Amnis ImageStream was supported by NIH Grant 1 S10 RR023458-01A1 (C Jordan PI).

Supported in part by the University of Rochester Developmental Center for AIDS Research grant P30 AI078498 (NIH/NIAID) and the University of Rochester School of Medicine and Dentistry.

D-CFAR web page

Amnis - ImageStream GenX

 

Amnis ImageStream, a hybrid fluorescent microscope/flow cytometer. With this technology, one can collect morphological data on cells stained with up to four different fluorochromes at 5,000 events per minute. Coupled with the analytical packages, IDEAS, statistical rigor can be brought to answer questions such as:

  • sub-cellular protein localization
  • surface maker co-localization
  • marker asymmetry
  • molecular trafficking
  • Phagocytosis ll

 

ImageStream GenX

Fee Structure

$50/hour

Configurations

Lasers include 405nm, 488nm, 594nm, and 658 nm

Channel Specifics

Channels 1-6 are primarily used for the 488nm laser. Channels 7-8 are primarily for the 405 laser, channels 9-10 for the 594nm laser, and 11-12 for the 658nm laser. There are multiple channels that have multiple or cross laser excitation that compensation will take care of. On the Fluorochrome List at the bottom of the page, the common dyes visible in each channel are listed, along with the secondary laser excitation. For more information, contact Matt Cochran or Wojtek.

  Channel 1 Channel 2 Channel 3 Channel 4 Channel 5 Channel 6
BP filter 467.5/75 532.5/55 577.5/35 627.5/65 700/80 770/60
Upper End

505

560 595 660 740 800
Peak 467.5 532.5 577.5 627.5 700 770
BP +/- 37.5 27.5 17.5 32.5 40 30
  Channel 7 Channel 8 Channel 9 Channel 10 Channel 11 Channel 12
BP filter 467.5/75 532.5/55 577.5/35 627.5/65 700/80 770/60
Upper End

505

560 595 660 740 800
Peak 467.5 532.5 577.5 627.5 700 770
BP +/- 37.5 27.5 17.5 32.5 40 30

Full Fluorchrome List

Use the following link to access the full list of fluors usable on this instrument. If a fluor you would like to use is not listed, contact Matt Cochran or Wojtek in the Flow Core.

Flurochrome List

Upcoming Events

Intro Lecture:

Location/Time - Rm 3-4103 @ 4PM (in the flow core hallway)

Schedule: Nov 10th and 24th.

Lecture series

next lecture coming soon!

 

Featured Article

Niswander et al., Cytometry

 

Spectral Viewers

Spectra Viewer (via Invitrogen)

Spectra Viewer (via BD Biosciences)

Spectra Viewer (via EBioscience)

 

Instrument Guidelines

Read Me here!

 

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