| Our work focuses on the role of the regulation of hepatic
metabolism of lipoproteins especially as related to regulatory
alterations associated with atherogenesis. A major effort in
this laboratory involves studies of the role of biochemical
hepatocellular heterogeneity in the metabolism of lipoproteins.
Studies to date, especially utilizing flow cytometry, have demonstrated
cellular heterogeneity of LDL uptake by rat liver through the
use of preparations labeled with a fluorescent probe developed
in our laboratory for this purpose, and cellular heterogeneity
in apo B expression in rat liver by intracellular immunocytochemical
fluorescence techniques. These studies provide basic information
regarding regulation of lipoprotein metabolism in the liver
but, more directly, they suggest the notion that recruitment
of hepatocellular subpopulations that are normally quiescent
with regard to lipoprotein metabolism could be helpful for normalization
of aberrant plasma lipoproteins. We are extending these studies
using plasma membrane markers of hepatocyte heterogeneity to
further characterize alterations in metabolism at the molecular
level. Additional projects in our laboratory include characterization
of a newly developed rat model of NIDDM, the Zucker diabetic
fatty (ZDF) rat, especially as related to gender differences
in regard to the development of NIDDM in response to dietary
fats; and studies of possible roles of cellular heterogeneity
among circulating leukocytes, especially monocytes, in atherogenesis.
|
Sparks JD, Shaw WN, Corsetti JP, Bolognino
M, Pesek JF, Sparks CE. Insulin-treated
Zucker diabetic fatty rats retain the hypertriglyceridemia
associated with obesity.
Metabolism. 2000 Nov;49(11):1424-30.
Chirieac DV, Chirieac LR, Corsetti JP, Cianci J, Sparks
CE, Sparks JD. Glucose-stimulated
insulin secretion suppresses hepatic triglyceride-rich lipoprotein
and apoB production.
Am J
Physiol Endocrinol Metab. 2000 Nov;279(5):E1003-11.
Corsetti
JP, Sparks JD, Peterson RG, Smith RL, Sparks CE. Effect
of dietary fat on the development of non-insulin dependent
diabetes mellitus in obese Zucker diabetic fatty
male and female rats. Atherosclerosis. 2000 Feb;148(2):231-41.
Sparks JD, Phung TL, Bolognino M, Cianci J, Khurana R, Peterson
RG, Sowden MP, Corsetti JP, Sparks CE. Lipoprotein
alterations in 10- and 20-week-old Zucker diabetic fatty
rats: hyperinsulinemic
versus insulinopenic hyperglycemia.
Metabolism. 1998 Nov;47(11):1315-24.
Lazova RZ, Sterry JA, Corsetti JP. Pseudoparaproteinemia
due to hemoglobinopathy. Clin Chem. 1995 Sep;41(9):1321-2.
No abstract available.
Sparks JD, Corsetti JP, Sparks CE. Liver
regrowth and apolipoprotein B secretion by rat hepatocytes
following partial hepatectomy.
Metabolism. 1994 Jun;43(6):681-90.
Corsetti JP, Cox C, Schulz
TJ, Arvan DA. Combined
serum amylase and lipase determinations for diagnosis of
suspected
acute
pancreatitis. Clin Chem. 1993 Dec;39(12):2495-9.
Corsetti JP, Sparks JD, Sikora B, Sparks CE. Cellular
heterogeneity in binding and uptake of low-density lipoprotein
in primary
rat hepatocytes. Hepatology. 1993
Apr;17(4):645-50.
Corsetti JP, Way BA, Sparks CE, Sparks JD. Immunolocalization,
quantitation and cellular heterogeneity of apolipoprotein
B in rat hepatocytes. Hepatology. 1992
Jun;15(6):1117-24.
Corsetti JP, Sterry JA, Sparks JD, Sparks CE, Weintraub
M. Effect
of weight loss on serum lipoprotein(a) concentrations in
an obese population. Clin Chem. 1991 Jul;37(7):1191-5.
Links are to the National Library of Medicine's PubMed publication
database.
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