UR Faculty: Paul D. Coleman

Ph.D. (1953) University of Rochester	Paul D. Coleman Professor of Neurobiology and Anatomy Primary Appointment: Neurobiology and Anatomy
Research: Neurobiology of Aging and Alzheimer's Disease Profiling expressions of multiple messages from single defined neurons in human and animal brains
Contact Information: E-Mail: paul_coleman@urmc.rochester.edu
University of Rochester School of Medicine and Dentistry 601 Elmwood Ave, Box 645 Rochester, NY 14642	Medical Center Room 1-9631 Phone: (585) 275-7704

Research Overview

The study of molecular biology of the brain has been limited by methods that take sizeable pieces of tissue, grind them up and perform biological studies. However, we know that within any, even very small sample of tissue are cells of many different types, and in the case of neurodegenerative disease, in many different stages of disease progression. These facts argued for examining single cells in aging and neurodegenerative disease. To do this we use methods of immunochemistry, in situ hybridization, and combinations of these two. In addition, we have recently developed methods for extracting messenger RNA from single identified cells, amplifying this message a million fold and determining the levels of expression of multiple genes from each single cell. The resulting masses of data are analyzed using advanced methods of multi-variate statistics and bio-informatics. Studies using these methods have demonstrated the re-expression of some cell cycle genes in neurons from the Alzheimer's diseased brain.

Recent Publications

Pub Med Citations
Chang J.W., Young D.A., Coleman P.D., and O'Banion, M.K. 2001, Two-dimensional gel analysis of secreted proteins induced by interleukin-1 beta in rat astrocytes. Neurochem. Int., 39(5-6):349-59.

Yao, P. J., Weimer, J. M., O'Herron, T. M., and Coleman, P.D. 2000, Clathrin assembly protein AP-2 is detected in both neuron and glia, and its reduction is prominent of layer II of frontal cortex in Alzheimer's disease. Neurobiol. of Aging, 21: 921.

Utal, A.K., Stopka, A.L., Roy, M. and Coleman, P.D. 1998, PEP-19 Immunohistochemistry Defines the Basal Banglia and Associated Structures in the Adult Human Brain, and is dramatically Reduced in Huntington's Disease. Neurosci. Vol. 86, No. 4, pp. 1055-1063.

Chow, N., Cox, C, Callahan, L.M, Weimer,J.L, Guom L. and Coleman, P.D. 1998, Expression profiles of multiple genes in single neurons of Alzheimer's disease. PNAS Vol. 95, pp. 9620-9625.

Yao, P.J. and Coleman, P.D. 1998, Reduced O-Glycosylated Clathrin assembly protein AP180: implication for synaptic vesicle recycling dysfunction in Alzheimer's disease. Neuosci. Lett., 252:33-36.

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