Yule Lab
David I. Yule, Ph.D.
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Research Overview
In this laboratory, we are studying intracellular calcium signaling in cells which are
typically, electrically non-excitable. In cells such as the liver, exocrine, pancreas, salivary glands and various cells in the blood, increases in intracellular calcium are fundamentally important for diverse processes including secretion of digestive enzymes and fluid, glucose metabolism together with cellular growth and differentiation. An important event in triggering an elevation in intracellular calcium is the activation of intracellular Ca2+ release channels in the endoplasmic reticulum. In most cells we study, these channels come in two "flavors"--the inositol 1,4,5 trisphosphate receptor (IP3R) and the ryanodine receptor (RyR). We are interested in how these channels are regulated, inter-react and ultimately define the types of calcium signals we observe. To these ends, we use a variety of state of the art imaging and electrophysiological techniques, including high speed digital imaging, confocal microscopy and whole cell patch-clamp to monitor calcium signals with high spatial and temporal resolution. The hope is that a better understanding of the mechanisms which underlie these important signals will give insight into the control of important physiological processes in both normal physiology and disease states.
Current projects in the lab are:
1. Determining the mechanisms whereby different agonists using traditionally the same intracellular messengers can generate agonist specific calcium signals and activate specific cellular processes.
2. Investigating if a genetic defect in the calcium signaling machinery expressed in salivary gland cells is responsible for patients with some forms of "dry mouth' disease.
3. Investigating defects in calcium signaling during acute pancreatitis.
Recent Publications
Almassy J, Won JH, Begenisich TB, and Yule DI. (2012) Apical Ca2+-activated potassium channels in mouse parotid acinar cells. J. Gen. Physiol. 139:121-133.
Park HS, Betzenhauser MJ, Zhang Y, and Yule DI. (2012) Regulation of Ca2+ release through inositol 1,4,5-trisphosphate receptors by adenine nucleotides in parotid acinar cells. Am. J. Physiol. Gastrointest. Liver Physiol. 302:G97-G104.
Won JH, Zhang Y, Ji B, Longsdon CD, and Yule DI. (2011) Phenotypic changes in mouse pancreatic stellate cell Ca2+ signaling events following activation in culture and in a disease model of pancreatitis. Mol. Biol. Cell. 22:421-436.
Masuda W, Betzenhauser MJ, and Yule DI. (2010) InsP3R-associated cGMP kinase substrate determines inositol 1,4,5-trisphosphate receptor susceptibility to phosphoregulation by cyclic nucleotide-dependent kinases. J. Biol. Chem. 285:37927-37938.
Yule DI. (2010) Pancreatic acinar cells: molecular insight from studies of signal-transduction using transgenic animals. Int. J. Biochem. Cell. Biol. 42:1757-1761.
Palk L, Sneyd J, Shuttleworth TJ, Yule DI, and Crampin EJ. (2010) A dynamic model of saliva secretion. J. Theor. Biol. 266:625-640.
Contact Us
David I. Yule, Ph.D.
University of Rochester
School of Medicine and Dentistry
Box 711
601 Elmwood Avenue
Rochester, NY 14642
Telephone: 585-273-2154
Fax: 585-273-2652
