Instruments

MALDI Mass Spectrometers

AutoflexIII TOF/TOFAutoflexIII TOF/TOF with smartbeam laser

The autoflexIII is a high-throughput platform for sample analysis. Multiple fragmentation types (LID, CID) coupled with the wide mass range of this instrument make it an ideal choice for analyzing carbohydrates and polymers as well as whole proteins and tryptic digests (peptides) that are fairly simple in complexity. Peptide mass fingerprint analysis and MS/MS analysis are carried out using Bruker's Compass software suite and by using MASCOT to search various databases. More Information on autoflexIII

Liquid Chromatography Mass Spectrometers (LC/MS)

LTQ LC/MS Linear Ion TrapLTQ LC/MS Linear Ion Trap

The LTQ Ion Trap utilizes a chromatographic timescale to separate complex mixtures. In addition to this instruments ability to separate samples, it offers Automatic Gain Control to ensure the ion trap is filled with the same number of ions for each scan. This makes this instrument an ideal choice for samples that could potentially contain more than one protein. Sample data analysis is carried out using BioWorks and Sequest database searching software. More Information on the newest generation LTQ

LCQ DECA XP MAX LC/MS 3D Ion TrapLCQ DECA XP MAX LC/MS 3D Ion Trap

The LCQ Ion Trap utilizes a chromatographic timescale, like the LTQ, to separate complex mixtures. This instrument also features AGC. Although it is slightly less sensitive than the LTQ, it still functions well with higher concentration protein/peptide samples. More Information on the newest generation LCQ

Apex Ultra 9.4T FT-ICRMS with Apollo II dual source

The strength of this instrument is "top-down" proteomics, where intact proteins are analyzed with multiple fragmentation types (ECD, CID and IRMPD) to characterize PTMs as well as gather sequence information. The dual source allows for typical data dependent ESI analysis and intermediate pressure MALDI analysis. The system is highlighted by its sub-ppm mass accuracy, a wide mass range (100-10,000 m/z) and greater than 1,500,000 resolution (FWHM) at 400 m/z. More Information

micrOTOF-Q

This spray TOF instrument is ideally equiped for the needs of proteomics as well as metabolomics and drug development (mass range 20-40,000 m/z coupled with a quadrupole mass filter and quadrupole collision cell). The resolution exceeds 17,500 (FWHM) with better than 3ppm mass accuracy, allowing for de novo sequencing. The speed of this instrument make it an ideal choice for automation of LC/MS workflow. More Information on micrOTOF-Q

Separation Devices

ProteomeLab PF 2DProteomeLab PF 2D

The ProteomeLab PF 2D is an automated, two-dimensional system designed to fractionate complex protein mixtures by pI and hydrophobicity. High loading capacity (up to 5mg) improves detection of lower-abundance proteins, while high resolution capability eliminates high-load band distortion typical of gel electrophoresis separation techniques. Entirely liquid flow and fraction collection increases the likelihood of membrane and hydrophobic protein identification when combined with mass spectrometry analysis. All first and second dimension fractions can be stored for future analysis at -80° C for up to a year. More Information on the ProteomeLab PF 2D

ProteomeLab PPSProteomeLab PPS

The ProteomeLab PPS takes advantage of the Beckman-Coulter IgY chemistry technology to partition serum, plasma, and cerebrospinal fluid into higher and lower abundance protein fractions. Two IgY chemistries are available: IgY-12 will remove the twelve most abundant proteins from human/primate biofluids, and IgY-R7 will remove the seven most abundant proteins from rodent biofluids. All fractions are collected and concentrated for further analysis. More Information on the ProteomeLab PPS

ProteomeLab PA 800 Capillary ElectrophoresisProteomeLab PA 800 Capillary Electrophoresis

The PA 800 has the ability to resolve and quantify proteins by pI or molecular weight. Its high resolution capabailities allow not only high resolution peptide maps, but carbohydrate profiles as well. The ability to interchange between a PDA detector and LIF detector as well as the various sampling formats (96 well plates, microfuge tubes, pcr vials and 2mL vials) make this a versatile characterization instrument. More Information on the PA 800

HPLC-Robot-Spotter in-line with MALDI

An Agilent Technilogies 1200 HPLC is configured in-line with a Bruker Daltonics Proteineer fc Robot Spotter. Therefore, complex peptide mixtures can be separated by reverse phase chromatography and all eluted fractions are directly applied to 384 spots on a Pac386 AnchorChip MALDI plate target. Automated sample fractionation by reverse phase reduces sample complexity, while automated MALDI plate spotting and TOF/TOF analysis will maximize indentification and coverage of complex protein/peptide samples. More information on the HPLC-Robot-Spotter

Research Instrumentation

The instruments in this section are available for use on special projects.

Ciphergen SELDI PBSII Mass SpectrometerCiphergen SELDI PBSII Mass Spectrometer

Surface Enhanced Laser Desorption Ionization mass spectrometry allows for on chip enrichment and purification of a wide variety of sample types.

Other Instruments

Sutter 2000/F Laser PullerSutter 2000/F Laser Puller

This instrument uses a CO2 Laser to create capillary columns for our LTQ and LCQ LC/MS systems. More Information the Sutter 2000/F Laser Puller

Coming Soon!

ImagePrepImagePrep

This sample preparation system combines with our AutoflexIII TOF/TOF. The instrument sprays matrix on tissue samples, which can then be analyzed on the MALDI to create high resolution images (50 micron). The result is a pseudo "stained" looking image of where proteins/peptides are in a particular tissue sample. The value of this is in identifying biomarkers and drug candidates. More Information on the ImagePrep