Protein Identifications

Method Number

10 20 30 40 50
Method Name MALDI-TOF MALDI-TOF/TOF LC-MALDI LC-ESI-MS/MS
Instrument AutoFlexIII AutoFlexIII RP-HPLC-
-robot-spotter-AutoFlex		 LTQ LCQ
Cost (per sample) $50 $150 $350 $200 $175
Sample requirements Protein solution, or bring in-gel Single band, pure
1 µg, Coomassie stained		 low complexity mixture w/ major components in pmole levels Complex mixture
or low protein bands
Expected results MW determination guaranteed protein ID
2 or more peptides with MSMS Score		 ID top 7 or more proteins
-high Mascot scores		 identifications of major and minor components & detection of low sample quantities
Bulk (>4) sample pricing $40 for each additional sample $125, for each additional sample $280, for each additional sample $150, for each additional sample
Turnaround time 5-10 days 5-10 days 5-10 days 5-10 days
Requirements for Guaranteed results
  1. Need 1 µg of target protein, that is relatively pure
  2. Core must cut out gel spots: Gel MUST BE stained with Coomassie blue
  3. Customer must consult with directors on experimental design
Enhanced Analysis Package

(Method 35)
  1. For maximal coverage of one protein sample
  2. Analysis is run on multiple instruments, with multiple runs, as necessary
  3. Cost is $500 to maximize coverage for pure proteins, present at µg levels
  4. Add extra $20 for automated phospho-screen
Preliminary Phosphorylation Screen (Method 21)
  1. This uses identical sample processing as Method 20
  2. Additional attention is used to search for post-translational modifications (PTMs)

For superior PTM analysis, see Methods 60, 70, 80, 90, 200, 210.
Preliminary Methylation Screen (Method 22)
  1. This uses identical sample processing as Method 20
  2. Additional attention is used to search for post-translational modifications (PTMs)

For superior PTM analysis, see Methods 60, 70, 80, 90, 200, 210.