M Anwar Iqbal, Ph.D.
India-Osmania University, Gandhi Medical College
Department of Pathology and Laboratory Medicine
The Iqbal Laboratory provides microarray comparative genomic hybridization (microarray CGH) testing for postnatal, prenatal and cancer patients. As part of URMC’s comprehensive pathology and laboratory medicine department, the lab helps clinical geneticists, reproductive geneticists, pediatric geneticists, neonatologists, clinical oncologists and haematopathologists provide the best patient care management.
The microarray CGH lab receives peripheral blood, amniotic fluid, chorionic villus, products of conception, bone marrow, formalin-fixed, paraffin embedded (FFPE) and solid tumor samples for DNA microarray testing. Testing determines the loss or gain of DNA segments in the patient compared to normal controls. These changes, known as copy number variations (CNVs), have been associated with susceptibility to certain disorders as well as reactions to drugs that treat these disorders.
Our associated research involves the following:
Evaluation of various CGH platforms and methodologies.
Studies of the efficacy of microarray-based testing for detection of genetic aberrations in various types of malignancies.
Microarray technology allows the comparison of a specific segment of DNA to thousands of probes. Procedural issues include questions such as, “What are the most useful probes for clinical testing, that yield the clearest results and point to possible therapies?” Data resulting from multiple tests of specific tumor types may also uncover new areas of genomic interest for basic researchers. Our laboratory is collaborating with Dr. Marilyn Li of Tulane University and with the CCMC in these areas.
The microarray lab is the only lab in the Upstate NY area providing in-house and reference testing for patients with autism, dysmorphic features and mental retardation, and for fetuses with multiple ultrasound abnormalities. This has allowed the accumulation of significant data for further studies.
Brigette Anne Brown-Kipphut, LiQiong Li, Nancy Wang and M. Anwar Iqbal. Comparison between targeted and targeted plus whole genome aCGH platforms – A pilot study of 35 cases. Am. Society of Human Genetics Annual Meeting, Philadelphia, Nov. 13-16, 2008 (Poster Presentation)
Wendy Chung, James Weisberger, Yi Sun, Pauline Brenholz, Stephanie Warren, Swaroop Aradhya, Charles LeDuc, Marc Grodman, Anwar Iqbal, John Bennett : Utility of oligonucleotide array comparative genomic hybridization to identify cryptic copy number alterations in myelodysplastic syndromes. ASH Meeting, San Francisco, CA Dec. 2008 (Published)
Jennifer Pryor, Brigette-Brown Kipphut, and M. Anwar Iqbal: High-resolution array-based comparative genomic hybridization detection of copy number changes in desmoplastic melanoma and malignant peripheral nerve sheath tumor. Am. Society of Human Genetics Annual Meeting, Hawaii, Oct. 26-29, 2009 (Poster Presentation)
Tracy Fuhrmann, Jessica Salamone, M. Anwar Iqbal, and Chin-To Fong. Chromosomal Microarray Analysis and the detection of a 15q11.2 deletion in a patient with Hypomelonosis of Ito. American College of Medical Genetics 2010 Annual Meeting, Albuquerque, NM (Poster Presentation)
Sei-Gyung Kim, Erin Lagoe, M. Anwar Iqbal, and Chin-To Fong. A Patient with complex dosage alterations including 7q11.23 micro deletion/duplication. American College of Medical Genetics 2010 Annual Meeting, Albuquerque, NM (Poster Presentation)
Athena M. Cherry, Marilyn L. Slovak, Lynda Campbell, Kathy Chun4, Virginie Eclache, Detlef Haase, Claudia Haferlach, Anne Hagemeijer, Barbara Hildebrandt, M. Anwar Iqbal, Suresh Jhanwar, Michelle LeBeau, Kazuma Ohyashiki, Francesc Sole, Daniel Van Dyke and Gordon Dewald. Will a Peripheral Blood (PB) Sample Yield the Same Diagnostic and Prognostic Cytogenetic Data as the Concomitant Bone Marrow (BM) In Myelodysplasia? An International Study Comparing Cytogenetics and Interphase FISH Using Parallel PB and BM Samples. ASH Meeting, Orlando, FL Dec. 2010 (Poster Presentation)
M.M. Li, X. Hu, H. Safah, A. Iqbal, and C. Lee. Clinical Application of Microarray Technology to Cancer: Are We Ready for Prime Time? Am. Society of Human Genetics Annual Meeting, Washington, DC, Nov. 3-6, 2010 (Platform Presentation)