Igor Kuzin, Ph.D. Research Interests Continued
There are several enhancer elements within IgH locus. One of the best characterized is intronic Em enhancer, which plays a role in regulation of gene transcription, VDJ recombination, somatic hypermutation, and immunoglobulin class switching. In their studies, to address the role of enhancer specificity in the regulation of IgH locus activity, they have created transgenic mice bearing either a deletion of the Em core element or replacement of the same element with the strong, ubiquitous SV40 transcriptional enhancer. The phenotype of these transgenic mice is currently under investigation.
The goal of the second project is to develop an artificial in vitro system for the induction of antibody immune response by cultivated human lymphocytes. They use specially designed bioreactors in which human lymphocytes isolated from donor tonsils are propagated, and explore various conditions that would be optimal for the induction of primary and secondary antibody immune responses to a variety of antigens. These studies have potential clinical application.
Andrea Bottaro, Ph.D.
University of Rochester
601 Elmwood Ave., Box 695
Rochester, NY 14642
Igor Kuzin, M.D., Ph.D.