Instruments

FV1000 Olympus Laser Scanning Confocal Microscope

FV1000Microscope of choice for the following studies:

  • Slides with fluorescent labels on cultured cells or tissue sections which require high resolution imaging
  • Samples up to 100 um thicknesses
  • Live imaging studies both short- and long-term including FRET, FRAP, FLIP, Photobleaching
  • Multiple time point ("mark and find" studies) and studies requiring image tiling
  • All fluorophores within the visible range with excitations beginning at 405nm can be imaged with this spectral laser confocal system. See below for laser lines.

Summary of Instrument Features

  • Microscope: Olympus IX81 (INVERTED microscope configuration)
  • Objectives: 4x, 10x, 20x oil, 40x oil, 60x oil, 100x oil, 60x water
  • DIC Capabilities: 20x, 40x, 60x, 100x
  • Lasers: 405 diode laser, 440 diode laser, 488/515 argon laser, 559 diode laser, 635 diode laser
  • Stage: Prior, Inc. motorized stage, includes tiling and multi-point mark and find memory function
  • Stage Holders: Multiple stage holder formats are provided by the Core. Current stage holders will support: 1 x 3 slides, 2 x 3 slides, circular (all sizes) glass-bottom culture dishes, well plates with glass-coverslip bottoms
  • Environmental Chamber: FULL STAGE INCUBATOR on the microscope, with CO2, temperature, and humidity regulation. Short- or long-term live imaging studies including FRET, FRAP, Multiple point timelapse imaging, etc. are possible
  • New Binder Incubator in room: Cultures can be equilibrated for a number of hours before being transferred into the stage incubator with identical C02, temp. and humidity conditions.
  • Service Contract Support: The FV1000 is under full service contract by Olympus.
  • Opportunities to work directly with Olympus reps: The core organizes multiple opportunities for PIs and staff to talk or work directly with the Olympus rep when he is on-campus.

 

Olympus VanoxAH-2 Conventional Microscope

Vanox AH-2Microscope of choice for the following studies:

  • Studies needing brightfield microscopy
  • Studies of samples with strong fluorophore signal needing standard conventional fluorescence imaging
  • Studies requiring "Live counting" image analysis

Summary of Instrument Features

  • Microscope: Upright microscope configuration
  • Objectives: 4x, 10x, 20x, 40x, 100x
  • Lamp source: Mercury Lamp
  • Stage: Prior, Inc. Motorized Stage
  • Rotatable slide holder: full rotation capabilities
  • Camera: MicroFire Camera
  • Image capture software: PictureFrame
  • Image analysis software: StereoInvestigator (version 8.0)
  • SI modules currently in Core:
    • Optical Fractionator
    • Confocal quantitation module
    • Space Balls module

 

High Definition Camera with Adjustable Photography Stand for Large Specimen Imaging

Large Specimen High Def CameraImaging equipment of choice for:

  • Studies requiring comparison of histologically processed sections to the original thick slice of tissue

Summary of Instrument Features

  • Stand: Kodak adjustable photography stand
  • Camera: SPOT Camera
  • Software: Image Pro Plus

 

Image Analysis Capabilities

At the current time, the following image analysis software packages are offered through the Core:

  • StereoInvestigator for quantitation of counts of cells using the Optical Fractionator method, version 8.0
  • Image Pro Plus
  • FV1000 Software - basic analysis of confocal images

Contact Us

Director:
Linda Callahan

Phone: 275-1317

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