Instruments

FV1000 Olympus Laser Scanning Confocal Microscope (NEW!)

FV1000 Microscope of choice for the following studies:

Slides with fluorescent labels on cultured cells or tissue sections which require high resolution imaging
Samples up to 100 um thicknesses
Live imaging studies both short- and long-term including FRET, FRAP, FLIP, Photobleaching
Multiple time point ("mark and find" studies) and studies requiring image tiling
All fluorophores within the visible range with excitations beginning at 405nm can be imaged with this spectral laser confocal system. See below for laser lines.

Microscope: Olympus IX81 (INVERTED microscope configuration)
Objectives: 4x, 10x, 20x oil, 40x oil, 60x oil, 100x oil, 60x water
DIC Capabilities: 20x, 40x, 60x, 100x
Lasers: 405 diode laser, 440 diode laser, 488/515 argon laser, 559 diode laser, 635 diode laser
Stage: Prior, Inc. motorized stage, includes tiling and multi-point mark and find memory function
Stage Holders: Multiple stage holder formats are provided by the Core. Current stage holders will support: 1 x 3 slides, 2 x 3 slides, circular (all sizes) glass-bottom culture dishes, well plates with glass-coverslip bottoms
Environmental Chamber: FULL STAGE INCUBATOR on the microscope, with CO2, temperature, and humidity regulation. Short- or long-term live imaging studies including FRET, FRAP, Multiple point timelapse imaging, etc. are possible
New Binder Incubator in room: Cultures can be equilibrated for a number of hours before being transferred into the stage incubator with identical C02, temp. and humidity conditions.
Service Contract Support: The FV1000 is under full service contract by Olympus.
Opportunities to work directly with Olympus reps: The core organizes multiple opportunities for PIs and staff to talk or work directly with the Olympus rep when he is on-campus.

Lecia Microscope of choice for the following studies:

Two- or three-color simulataneous imaging.
Currently supports both slide-based and live imaging studies that do not need environmental controls. Users should check directly with the Core director as to status of instrument before using.
The SP1 was originally purchased in 2000.

Microscope: Leica DMRE (Upright Microscope Configuration)
Objectives: 5x, 20x oil, 40x oil, 63x saline, 63x oil, 100x oil, 40x water immersion, 1.6x
DIC Capabilities: 40x, 63x, 100x
Lasers: 458/488 argon laser, 543 Helium/neon laser, 633 Helium/neon laser water immersion
Stage: Manual
Rotatable slide holder: full rotation capabilities

Vanox AH-2 Microscope of choice for the following studies:

Studies needing brightfield microscopy
Studies of samples with strong fluorophore signal needing standard conventional fluorescence imaging
Studies requiring "Live counting" image analysis

Microscope: Upright microscope configuration
Objectives: 4x, 10x, 20x, 40x, 100x
Lamp source: Mercury Lamp
Stage: Prior, Inc. Motorized Stage
Rotatable slide holder: full rotation capabilities
Camera: MicroFire Camera
Image capture software: PictureFrame
Image analysis software: StereoInvestigator (version 8.0)
SI modules currently in Core:
- Optical Fractionator
- Confocal quantitation module
- Space Balls module

Large Specimen High Def Camera Imaging equipment of choice for:

Studies requiring comparison of histologically processed sections to the original thick slice of tissue

At the current time, the following image analysis software packages are offered through the Core:

StereoInvestigator for quantitation of counts of cells using the Optical Fractionator method, version 8.0
Image Pro Plus
FV1000 Software - basic analysis of confocal images
Lecia SP1 software - basic analysis of confocal images

Director:
Linda Callahan
Phone: 275-1317

Lecture in 3-9654 on Wednesday 28 October - Introduction to Immunofluorescent Microscopy