Instruments
FV1000 Olympus Laser Scanning Confocal Microscope
Microscope of choice for the following studies:
- Slides with fluorescent labels on cultured cells or tissue sections which require high resolution imaging
- Samples up to 100 um thicknesses
- Live imaging studies both short- and long-term including FRET, FRAP, FLIP, Photobleaching
- Multiple time point ("mark and find" studies) and studies requiring image tiling
- All fluorophores within the visible range with excitations beginning at 405nm can be imaged with this spectral laser confocal system. See below for laser lines.
Summary of Instrument Features
- Microscope: Olympus IX81 (INVERTED microscope configuration)
- Objectives: 4x, 10x, 20x oil, 40x oil, 60x oil, 100x oil, 60x water
- DIC Capabilities: 20x, 40x, 60x, 100x
- Lasers: 405 diode laser, 440 diode laser, 488/515 argon laser, 559 diode laser, 635 diode laser
- Stage: Prior, Inc. motorized stage, includes tiling and multi-point mark and find memory function
- Stage Holders: Multiple stage holder formats are provided by the Core. Current stage holders will support: 1 x 3 slides, 2 x 3 slides, circular (all sizes) glass-bottom culture dishes, well plates with glass-coverslip bottoms
- Environmental Chamber: FULL STAGE INCUBATOR on the microscope, with CO2, temperature, and humidity regulation. Short- or long-term live imaging studies including FRET, FRAP, Multiple point timelapse imaging, etc. are possible
- New Binder Incubator in room: Cultures can be equilibrated for a number of hours before being transferred into the stage incubator with identical C02, temp. and humidity conditions.
- Service Contract Support: The FV1000 is under full service contract by Olympus.
- Opportunities to work directly with Olympus reps: The core organizes multiple opportunities for PIs and staff to talk or work directly with the Olympus rep when he is on-campus.
Olympus VanoxAH-2 Conventional Microscope
Microscope of choice for the following studies:
- Studies needing brightfield microscopy
- Studies of samples with strong fluorophore signal needing standard conventional fluorescence imaging
- Studies requiring "Live counting" image analysis
Summary of Instrument Features
- Microscope: Upright microscope configuration
- Objectives: 4x, 10x, 20x, 40x, 100x
- Lamp source: Mercury Lamp
- Stage: Prior, Inc. Motorized Stage
- Rotatable slide holder: full rotation capabilities
- Camera: MicroFire Camera
- Image capture software: PictureFrame
- Image analysis software: StereoInvestigator (version 8.0)
- SI modules currently in Core:
- Optical Fractionator
- Confocal quantitation module
- Space Balls module
High Definition Camera with Adjustable Photography Stand for Large Specimen Imaging
Imaging equipment of choice for:
- Studies requiring comparison of histologically processed sections to the original thick slice of tissue
Summary of Instrument Features
- Stand: Kodak adjustable photography stand
- Camera: SPOT Camera
- Software: Image Pro Plus
Image Analysis Capabilities
At the current time, the following image analysis software packages are offered through the Core:
- StereoInvestigator for quantitation of counts of cells using the Optical Fractionator method, version 8.0
- Image Pro Plus
- FV1000 Software - basic analysis of confocal images
Contact Us
Director:
Linda Callahan
Phone: 275-1317
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