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Paul Kammermeier

TitleAssociate Professor
InstitutionSchool of Medicine and Dentistry
DepartmentPharmacology and Physiology
AddressUniversity of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 711
Rochester NY 14642
 
 Overview
My research interests center around G protein-coupled receptor function, particularly metabotropic glutamate receptors (mGluRs), and ion channel modulation in neurons. Naturally, this work has led me toward an interest in heterotrimeric G proteins, G protein-coupled receptors and factors that modulate G protein signaling, such as the Regulators of G protein Signaling (RGS) proteins (see Kammermeier and Ikeda, 1999, Neuron 22:819). I have also become interested in the Homer family of scaffolding proteins, which I have shown to be important in the function and sub-cellular localization of metabotropic glutamate receptors (Kammermeier et al., 2000, Journal of Neuroscience 220:7238; Kammermeier, 2005, BMC Neuroscience 7:1). In the long term, my goal is to understand the mechanisms of regulation, localization and signaling that impart specificity to the various mGluRs, and how regulation of mGluR signaling alters synaptic strength in the nervous system.
In my laboratory, two basic experimental approaches are used. The first utilizes primary cultures of isolated SCG neurons for electrophysiological, molecular biological, immunofluorescence and RT-PCR experiments. The main technique used in this system is to examine the well-characterized ion channel modulatory pathways using patch-clamp electro-physiology as an assay for G protein coupled receptor activity. The neurons can be made to express various heterologous proteins (including mGluRs) via intranuclear injection of cDNA. I have recently used this system to examine mGluR-G protein coupling specificity, mGluR desensitization and the regulation of mGluR signaling and trafficking by various associated proteins.

The second approach is to use native neuronal cells. Currently, we are working with both primary isolated ON Bipolar cells from the rat retina and with rat hippocampal neurons to examine G protein coupling of mGluR6 and the effects of Homer protein regulation of mGluR5 at synapses, respectively. These systems allow us to ask questions about the physiological relevance in parallel with the more tractable, highly amenable SCG system described above.

Finally, we are beginning to incorporate other fluorescence-based techniques such as total internal reflection fluorescence (TIRF) microscopy (Kammermeier, 2005, BMC Neuroscience 7:1) to examine protein distribution in or very near the cell membrane.

 
 Selected Publications
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  1. Hu JH, Yang L, Kammermeier PJ, Moore CG, Paul R B, Tu J, Yu S, Petralia RS, Li Z, Zhang PW, Park JM, Dong X, Xiao B, Worley PF. Preso1 dynamically regulates group I metabotropic glutamate receptors. Nat Neurosci. 2012 Jun; 15(6):836-44.
    View in: PubMed
  2. Kammermeier PJ. Functional and pharmacological characteristics of metabotropic glutamate receptors 2/4 heterodimers. Mol Pharmacol. 2012 Sep; 82(3):438-47.
    View in: PubMed
  3. Beqollari D, Kammermeier PJ. The interaction between mGluR1 and the calcium channel Ca(V2.1) preserves coupling in the presence of long Homer proteins. Neuropharmacology. 2013 Mar; 66:302-10.
    View in: PubMed
  4. Kammermeier PJ. The orthosteric agonist 2-chloro-5-hydroxyphenylglycine activates mGluR5 and mGluR1 with similar efficacy and potency. BMC Pharmacol. 2012; 12:6.
    View in: PubMed
  5. Beqollari D, Kammermeier PJ. Venus fly trap domain of mGluR1 functions as a dominant negative against group I mGluR signaling. J Neurophysiol. 2010 Jul; 104(1):439-48.
    View in: PubMed
  6. Kammermeier PJ. C-terminal deletion of metabotropic glutamate receptor 1 selectively abolishes coupling to Galphaq. Eur J Pharmacol. 2010 Feb 10; 627(1-3):63-8.
    View in: PubMed
  7. Beqollari D, Betzenhauser MJ, Kammermeier PJ. Altered G-protein coupling in an mGluR6 point mutant associated with congenital stationary night blindness. Mol Pharmacol. 2009 Nov; 76(5):992-7.
    View in: PubMed
  8. Cao Y, Masuho I, Okawa H, Xie K, Asami J, Kammermeier PJ, Maddox DM, Furukawa T, Inoue T, Sampath AP, Martemyanov KA. Retina-specific GTPase accelerator RGS11/G beta 5S/R9AP is a constitutive heterotrimer selectively targeted to mGluR6 in ON-bipolar neurons. J Neurosci. 2009 Jul 22; 29(29):9301-13.
    View in: PubMed
  9. Kammermeier PJ. Endogenous homer proteins regulate metabotropic glutamate receptor signaling in neurons. J Neurosci. 2008 Aug 20; 28(34):8560-7.
    View in: PubMed
  10. Beqollari D, Kammermeier PJ. The mGlu(4) receptor allosteric modulator N-phenyl-7-(hydroxyimino)cyclopropa[b]chromen-1a-carboxamide acts as a direct agonist at mGlu(6) receptors. Eur J Pharmacol. 2008 Jul 28; 589(1-3):49-52.
    View in: PubMed
  11. Kammermeier PJ, Worley PF. Homer 1a uncouples metabotropic glutamate receptor 5 from postsynaptic effectors. Proc Natl Acad Sci U S A. 2007 Apr 3; 104(14):6055-60.
    View in: PubMed
  12. Tian L, Kammermeier PJ. G protein coupling profile of mGluR6 and expression of G alpha proteins in retinal ON bipolar cells. Vis Neurosci. 2006 Nov-Dec; 23(6):909-16.
    View in: PubMed
  13. Kammermeier PJ. Surface clustering of metabotropic glutamate receptor 1 induced by long Homer proteins. BMC Neurosci. 2006; 7:1.
    View in: PubMed
  14. Kammermeier PJ, Yun J. Activation of metabotropic glutamate receptor 1 dimers requires glutamate binding in both subunits. J Pharmacol Exp Ther. 2005 Feb; 312(2):502-8.
    View in: PubMed
  15. Kammermeier PJ, Davis MI, Ikeda SR. Specificity of metabotropic glutamate receptor 2 coupling to G proteins. Mol Pharmacol. 2003 Jan; 63(1):183-91.
    View in: PubMed
  16. Kammermeier PJ, Ikeda SR. Metabotropic glutamate receptor expression in the rat superior cervical ganglion. Neurosci Lett. 2002 Sep 27; 330(3):260-4.
    View in: PubMed
  17. Ikeda SR, Kammermeier PJ. M current mystery messenger revealed? Neuron. 2002 Aug 1; 35(3):411-2.
    View in: PubMed
  18. Kammermeier PJ, Ikeda SR. Desensitization of group I metabotropic glutamate receptors in rat sympathetic neurons. J Neurophysiol. 2002 Apr; 87(4):1669-76.
    View in: PubMed
  19. Kammermeier PJ, Ikeda SR. A role for Seven in Absentia Homolog (Siah1a) in metabotropic glutamate receptor signaling. BMC Neurosci. 2001; 2:15.
    View in: PubMed
  20. Kammermeier PJ, Xiao B, Tu JC, Worley PF, Ikeda SR. Homer proteins regulate coupling of group I metabotropic glutamate receptors to N-type calcium and M-type potassium channels. J Neurosci. 2000 Oct 1; 20(19):7238-45.
    View in: PubMed
  21. Kammermeier PJ, Ruiz-Velasco V, Ikeda SR. A voltage-independent calcium current inhibitory pathway activated by muscarinic agonists in rat sympathetic neurons requires both Galpha q/11 and Gbeta gamma. J Neurosci. 2000 Aug 1; 20(15):5623-9.
    View in: PubMed
  22. Ikeda SR, Jeong SW, Kammermeier PJ, Ruiz-Velasco V, King MM. Heterologous expression of a green fluorescent protein-pertussis toxin S1 subunit fusion construct disrupts calcium channel modulation in rat superior cervical ganglion neurons. Neurosci Lett. 1999 Aug 27; 271(3):163-6.
    View in: PubMed
  23. Kammermeier PJ, Ikeda SR. Expression of RGS2 alters the coupling of metabotropic glutamate receptor 1a to M-type K+ and N-type Ca2+ channels. Neuron. 1999 Apr; 22(4):819-29.
    View in: PubMed
  24. Kammermeier PJ, Jones SW. Facilitation of L-type calcium current in thalamic neurons. J Neurophysiol. 1998 Jan; 79(1):410-7.
    View in: PubMed
  25. Kammermeier PJ, Jones SW. High-voltage-activated calcium currents in neurons acutely isolated from the ventrobasal nucleus of the rat thalamus. J Neurophysiol. 1997 Jan; 77(1):465-75.
    View in: PubMed
  26. Elmslie KS, Kammermeier PJ, Jones SW. Reevaluation of Ca2+ channel types and their modulation in bullfrog sympathetic neurons. Neuron. 1994 Jul; 13(1):217-28.
    View in: PubMed
  27. Elmslie KS, Kammermeier PJ, Jones SW. Calcium current modulation in frog sympathetic neurones: L-current is relatively insensitive to neurotransmitters. J Physiol. 1992 Oct; 456:107-23.
    View in: PubMed

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