Response of the Hematopoietic System to Ionizing Radiation
Radiation causes bone marrow damage and anemia, however, the response of the erythroid lineage to irradiation has not been well characterized. We have developed a novel method to quantify erythroid precursors using multispectral imaging flow cytometry (McGrath, et al. J Immunol Methods, 2008). Our preliminary studies indicate that erythroid progenitors and precursors are extremely sensitive targets of radiation damage and recover in an oscillatory pattern suggesting the operation of negative feedback regulation. We are currently investigating the potential role of directed cytokine therapy to mitigate damage and enhance the recovery of hematopoietic cells following a bioterrorist attack or nuclear disaster.
Differential response of early-stage erythroid progenitors (BFU-E) and late-stage erythroid progenitors (CFU-E) to a single dose of 4 Gy radiation.
McGrath KE, Bushnell T, Palis J. Multispectral imaging of hematopoietic cells: where flow meets morphology. J Immunol Methods 336:91-97, 2008.
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, Chen Y, Williams JP, McGrath KE, Dertinger SD, Palis J. Sublethal radiation injury uncovers a functional transition during erythroid maturation. Experimental Hematology, 39:434-445, 2011.
Peslak SA, Wenger J, Bemis JC, Kingsley PD, Koniski AD, McGrath KE, Palis J. EPO-mediated expansion of late-stage erythroid progenitors in the bone marrow initiates recovery from sublethal radiation stress. Blood 120:2501-2511, 2012.