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Sergiy Nadtochiy, Ph.D.

Sergiy Nadtochiy, Ph.D.


Call Center (585) 276-3000

About Me

Faculty Appointments

Assistant Professor - Department of Anesthesiology and Perioperative Medicine (SMD)

Assistant Professor - Department of Neuroscience (SMD) - Joint

Assistant Professor - Department of Pharmacology and Physiology (SMD) - Joint


Post-doctoral Training & Residency

Bogomoletz Institute of Physiology, Department of Blood Circulation, Ukraine; Post-Doctoral Fellow with Dr. Vadim Sagach;

Department of Anesthesiology, University of Rochester Medical Center, USA
Post-Doctoral Fellow with Dr. Paul Brookes; 2004 - 2009


PhD | Ukraine-Bogomoletz Institute of Physiology. Biophysics. 2004

MS | Ukraine-Kyiv Taras Shevchenko University. Radiophysics. 2000

BS | Ukraine-Kyiv Taras Shevchenko University. Applied Physics. 1999


URSMD Keith Miner Ford Award for Excellence in Teaching. 2022

URSMD Keith Miner Ford Award for Excellence in Teaching. 2021

The Manual D. Goldman Prize. 2021

URSMD Keith Miner Ford Award for Excellence in Teaching. 2020

Teaching commendations for the contributions provided to the first year medical students. 2019

URSMD Alumni Association Gold Medal Award. 2018

The Manual D. Goldman Prize. 2017


Journal Articles

Intravenous dabigatran provides adequate anticoagulation for cardiopulmonary bypass using a rabbit model

Sergiy M Nadtochiy, Tatsiana Stefanos, Ronald E Angona, Nathan Darrow, Karen Jones, Changyong Feng, Mohamed Algahim, Dana LeMoine, Robin Westcott, Brian Anderson, Michael F Swartz, Michael P Eaton.

Anesthesiology. 2023; 138(5): 523-532.

Delayed concentration effect models for dabigatran anticoagulation

Eaton MP, Nadtochiy SM, Stefanos T, LeMoine D, Anderson BJ.

Paediatr Anaesth. 2022; 32(10): 1113-1120.

Distinct effects of intracellular vs. extracellular acidic pH on the cardiac metabolome during ischemia and reperfusion

Milliken AS, Ciesla JH, Nadtochiy SM, Brookes PS.

J Mol Cell Cardiol. 2022; 174: 101-114.

Inhibiting Succinate Release Worsens Cardiac Reperfusion Injury by Enhancing Mitochondrial Reactive Oxygen Species Generation

Alexander S Milliken, Sergiy M Nadtochiy, Paul S Brookes.

J Am Heart Assoc. 2022; e026135.

Rivaroxaban Reduces the Dabigatran Dose Required for Anticoagulation During Simulated Cardiopulmonary Bypass

Nadtochiy, Sergiy M.; Stefanos, Tatsiana; Angona, Ronald E.; Lebedko, Natalie; Baldzizhar, Aksana; Feng, Changyong; Eaton, Michael P.

Anesthesia & Analgesia. 2022; 135(1): 52-59.

High-Dose Dabigatran Is an Effective Anticoagulant for Simulated Cardiopulmonary Bypass Using Human Blood.

Nadtochiy, SM; Baldzizhar, A; Stefanos, T; et al.

Anesth Analg. 2021; 132(2): 566-574.

Nadtochiy, SM; Ngai, M; Brookes, PS.

Modified Blue Native Gel Approach for Analysis of Respiratory

ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.

Kulkarni, CA; Nadtochiy, SM; Kennedy, L; Brookes, PS; et al.

Elife. 2020; 9.

Cardioprotection by nicotinamide mononucleotide (NMN): Involvement of glycolysis and acidic pH.

Nadtochiy SM, Wang YT, Nehrke K, Munger J, Brookes PS

Journal of molecular and cellular cardiology.. 2018 August 121 :155-162. Epub 06/26/2018.

Cardiac metabolic effects of K1.2 channel deletion and evidence for its mitochondrial localization.

Smith CO, Wang YT, Nadtochiy SM, Miller JH, Jonas EA, Dirksen RT, Nehrke K, Brookes PS

FASEB journal : official publication of the Federation of American Societies for Experimental Biology.. 2018 June 4 :fj201800139R. Epub 06/04/2018.

Metabolomics reveals critical adrenergic regulatory checkpoints in glycolysis and pentose-phosphate pathways in embryonic heart.

Peoples JNR, Maxmillian T, Le Q, Nadtochiy SM, Brookes PS, Porter GA, Davidson VL, Ebert SN

The Journal of biological chemistry.. 2018 May 4293 (18):6925-6941. Epub 03/14/2018.

Potential mechanisms linking SIRT activity and hypoxic 2-hydroxyglutarate generation: no role for direct enzyme (de)acetylation.

Nadtochiy SM, Wang YT, Zhang J, Nehrke K, Schafer X, Welle K, Ghaemmaghami S, Munger J, Brookes PS

The Biochemical journal.. 2017 August 10474 (16):2829-2839. Epub 08/10/2017.

Acidic pH Is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling.

Nadtochiy SM, Schafer X, Fu D, Nehrke K, Munger J, Brookes PS

The Journal of biological chemistry.. 2016 September 16291 (38):20188-97. Epub 08/10/2016.

The cardioprotective compound cloxyquin uncouples mitochondria and induces autophagy.

Zhang J, Nadtochiy SM, Urciuoli WR, Brookes PS

American journal of physiology. Heart and circulatory physiology.. 2016 January 1310 (1):H29-38. Epub 10/30/2015.

Cyclophilin D Knock-Out Mice Show Enhanced Resistance to Osteoporosis and to Metabolic Changes Observed in Aging Bone.

Shum LC, White NS, Nadtochiy SM, Bentley KL, Brookes PS, Jonason JH, Eliseev RA

PloS one.. 2016 11 (5):e0155709. Epub 05/16/2016.

Metabolomic profiling of the heart during acute ischemic preconditioning reveals a role for SIRT1 in rapid cardioprotective metabolic adaptation

Nadtochiy SM, Urciuoli W, Zhang J, Schafer X, Munger J, Brookes PS.

J Mol Cell Cardiol. 2015; 88: 64-72.

The cardioprotective compound cloxyquin uncouples mitochondria and induces autophagy. Am J Physiol Heart Circ Physiol

Zhang J, Nadtochiy SM, Urciuoli WR, Brookes PS.

Am J Physiol Heart Circ Physiol. 2015; .

Ischaemic accumulation of succinate controls reperfusion injury through mitochondrial ROS

Edward T. Chouchani, Victoria R. Pell, Edoardo Gaude, Dunja Aksentijevi?, Stephanie Y. Sundier, Ellen L. Robb, Angela Logan, Sergiy M. Nadtochiy, Emily N. J. Ord, Anthony C. Smith, Filmon Eyassu, Rachel Shirley, Chou-Hui Hu, Anna J. Dare, Andrew M. James, Sebastian Rogatti, Richard C. Hartley, Simon Eaton, Ana S. H. Costa, Paul S. Brookes, Sean M. Davidson, Michael R. Duchen, Kourosh Saeb-Parsy, Michael J. Shattock, Alan J. Robinson, Lorraine M. Work, Christian Frezza, Thomas Krieg & Michael P. Murphy.

Nature. 2014; 515: 431-435.

SIRT3 deficiency exacerbates ischemia-reperfusion injury: implication for aged hearts

Porter GA, Urciuoli WR, Brookes PS, Nadtochiy SM. (.

Am J Physiol Heart Circ Physiol. 2014; 306: H1602-H1609.

Mitochondrially targeted nitro-linoleate: a new tool for the study of cardioprotection

Nadtochiy SM, Madukwe J, Hagen F, Brookes PS.

Br J Pharmacol. 2014; 171: 2091-2098.

Cardioprotection by S-nitrosation of a cysteine switch on mitochondrial complex I

Chouchani ET, Methner C, Nadtochiy SM, Logan A, Pell VR, Ding S, James AM, Cochemé HM, Reinhold J, Lilley KS, Partridge L, Fearnley IM, Robinson AJ, Hartley RC, Smith RA, Krieg T, Brookes PS, Murphy MP.

Nat Med. 2013; 19: 753-759.

A non-cardiomyocyte autonomous mechanism of cardioprotection involving the SLO1 BK channel

Wojtovich AP, Nadtochiy SM, Urciuoli WR, Smith CO, Grunnet M, Nehrke K, Brookes PS.

PeerJ. 2013; 1(e48.).

A cell-based phenotypic assay to identify cardioprotective agents

Guo S, Olm-Shipman A, Walters A, Urciuoli WR, Devito S, Nadtochiy SM, Wojtovich AP, Brookes PS.

Circ Res. 2012; 110: 948-57.

Nitroalkenes confer acute cardioprotection via adenine nucleotide translocase 1

Nadtochiy SM, Qiuyu Zhu, William Urciuoli, Ruslan Rafikov, Stephen M. Black, Paul S. Brookes.

J Biol Chem. 2012; 287: 3573-80.

SLO-2 Is Cytoprotective and Contributes to Mitochondrial Potassium Transport

Wojtovich AP, Sherman TA, Nadtochiy SM, Urciuoli WR, Brookes PS, Nehrke K.

PLoS One. 2011; 6: e28287.

SIRT1-mediated acute cardioprotection.

Nadtochiy SM, Yao H, McBurney MW, Gu W, Guarente L, Rahman I, Brookes PS.

Am J Physiol Heart Circ Physiol. 2011; 301: H1506-12.

p90 ribosomal S6 kinase regulates activity of the renin-angiotensin system: A pathogenic mechanism for ischemia-reperfusion injury

Shi X, Yan C, Nadtochiy SM, Abe JI, Brookes PS, Berk BC.

J Mol Cell Cardiol. 2011; 51: 272-5.

Redox Regulation of the Mitochondrial K(ATP) Channel in Cardioprotection

Queliconi BB, Wojtovich AP, Nadtochiy SM, Kowaltowski AJ, Brookes PS.

Biochim Biophys Acta. 2011; 1813: 1309-15.

Lysine Deacetylation in Ischemic Preconditioning: The Role of SIRT1

Nadtochiy SM, Redman E, Rahman I, Brookes PS.

Cardiovasc Res. 2011; 89: 643-9.

Identification of S-nitrosated mitochondrial proteins by S-nitrosothiol Difference In Gel Electrophoresis (SNO-DIGE): implications for the regulation of mitochondrial function by reversible S-nitrosation

Chouchani E, Hurd T, Nadtochiy SM, Brookes PS, Fearnley IM, Lilley KS, Smith RA, Murphy MP.

Biochem J. 2010; 430: 49-59.

Modulation of Cell Surface Protein Free Thiols; A Potential Novel Mechanism of Action of the Sesquiterpene Lactone Parthenolide.

Skalska J, Brookes PS, Nadtochiy SM, Hilchey S, Jordan C, Guzman G, Briehl M, Bernstein S.

PLoS One. 2009; 4: e8115.

A mitochondria-targeted S-nitrosothiol modulates respiration, nitrosates thiols, and protects against ischemia-reperfusion injury

Prime TA, Blaikie FH, Nadtochiy SM, James AM, Dahm CC, Vitturi D, Patel RP, Hiley CA, Abakumova I, Requejo R, Chouchani E, Hurd TR, Garvey J, Taylor TC, Brookes PS, Smith RAJ & Murphy MP.

Proc. Natl. Acad. Sci. USA. 2009; 106: 10764-10769.

In-vivo cardioprotection by S-nitroso-2-mercaptopropionyl glycine

Nadtochiy SM, Burwell LS, Ingraham CA, Pinkert CA, Brookes PS.

J Mol Cell Cardiol. 2009; 46: 960-968.

Detection and Quantification of Protein Adduction by Electrophilic Fatty Acids: Mitochondrial Generation of Fatty Acid Nitroalkene Derivatives.

Schopfer FJ, Batthyany C, Baker PR, Bonacci G, Cole MP, Rudolph V, Groeger AL, Rudolph TK, Nadtochiy SM, Brookes PS, Freeman BA.

Free Rad. Biol. Med. 2009; 46: 1250-9.

Mitochondrial nitroalkene formation and mild uncoupling in ischaemic preconditioning: implications for cardioprotection.

Nadtochiy SM, Baker PR, Freeman BA, Brookes PS.

Cardiovascular Research. 2009; 82: 333-40.

Purine release: A protective signaling mechanism of the mitochondrial permeability transition pore in ischemia

Nadtochiy SM, Nauduri D, Shymanskaya TV, Sagach VF, Brookes PS.

Fiziol Zh. 2008; 54(5-14.).

Sustained Weight Loss After Roux-en-Y Gastric Bypass Is Characterized by Down Regulation of Endocannabinoids and Mitochondrial Function.

Guijarro A, Osei-Hyiaman D, Harvey-White J, Kunos G, Suzuki S, Nadtochiy SM, Brookes PS, Meguid MM.

Ann Surg. 2008; 247: 779-790.

Characterization of weight loss and weight regain mechanisms after Roux-en-Y gastric bypass in rats.

Guijarro A, Suzuki S, Chen C, Kirchner H, Middleton FA, Nadtochiy SM, Brookes PS, Niijima A, Inui A, Meguid MM.

Am J Physiol Regul Integr Comp Physiol. 2007; 293: R1474-89.

Cardioprotection & mitochondrial S-nitrosation: effect of the mitochondrial S-nitrosating agent SNO-MPG in cardiac ischemia-reperfusion injury

Nadtochiy SM, Burwell LS, Brookes PS.

J Mol Cell Cardiol. 2007; 42: 812-825.

Different mechanisms of mitochondrial proton leak in ischaemia/reperfusion injury and preconditioning: implications for pathology and cardioprotection

Biochem. J. 2006; 395: 611-8.

Direct Evidence of Mitochondrial Complex I S-nitrosation.

Burwell LS, Nadtochiy SM, Tompkins AJ, Young SM, Brookes PS.

Biochem. J. 2006; 394: 627-34.

Effect of hypoxic preconditioning on the development of post-reperfusion disturbances of heart function and activation of mitochondrial permeability transition.

Sagach VF, Shimanskay TV, Nadtochiy SM, Dobrovolskiy FV.

Reports of the National Academy of Sciences of Ukraine. 2006; 3: 158-162.

Role of nitric oxide and mitochondrial permeability pore in changes of oxygen consumption in the working skeletal muscle.

Sagach VF, Bohuslavs'kyi AIu, Dmytriieva AV, Nadtochiy SM.

Fiziol Zh. 2004; 50: 19-26.

Factor released under heart reperfusion may be marker of opening of the mitochondrial permeability transition pore.

Sagach VF, Shymanskaya TV, Nadtochiy SM.

Fiziol. Zh. 2003; 49: 6-12.

Determination of the stable mitochondrial factor in vivo

Nadtochiy SM, Boguslavskiy AY, Sagach VF.

Fiziol Zh. 2003; 49: 25-31.

Protection of heart from reperfusion injury by annexin II

Sagach VF, Shimanskay TV, Nadtochiy SM, Babiychuk VS, Drager A.

Reports of the National Academy of Sciences of Ukraine. 2003; 9: 170-177.

Factor released under heart reperfusion; investigation of its effect on myocardium, coronary and peripheral vessels.

Sagach VF, Dmitrieva AV, Shimanskay TV, Nadtochiy SM.

Fiziol. Zh. 2002; 48: 3-8.

Protection of heart from reperfusion injury and ineffective oxygen consumption by inhibitors of the mitochondrial permeability transition pore

Sagach VF, Shymanskaya TV, Nadtochiy SM.

Fiziol. Zh. 2002; 48: 3-9.

The role of nitric oxide in the changes of oxygen consumption and oxygen cost of myocardial work.

Sagach VF, Shimanskay TV, Nadtochiy SM.

Fiziol. Zh. 2000; 46: 33-38.


Methods in Molecular Biology (2020)

Chapter: Modified and improved approach for the supercomplexes extraction, visualization and in-gel Cx V assay.

Authors: Nadtochiy SM, Ngai M, Brookes PS.

Publisher: Springer Science+Business Media New York. 2020

Free Radical Effects on Membranes (2008)

Chapter: The Interaction of Mitochondrial Membranes with Reactive Oxygen and Nitrogen Species

Authors: Paul Brookes, Andrew Wojtovich, Lindsay Burwell, Davide Hoffman and Sergiy Nadtochiy

Publisher: Academic Press, New York 2008