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Karen L. Bentley, M.S.

Contact Information

Phone Numbers

Office: (585) 275-1954

Research Labs

The purpose of the URMC Electron Microscope Research Core Facility is to provide up-to-date and cost-effective support to researchers whose studies involve the use of electron microscopy.

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Biography

Professional Background

Ms.(de Mesy) Bentley has 29 years of experience in Ultrastructural Pathology and is considered an expert in both diagnostic and research electron microscopy. She served as the Associate Director of the Diagnostic Electron Microscope (EM) Laboratory with P. Anthony di Sant'Agnese, M.D., a surgical pathologist, who was the Director. Together they developed a month long resident Diagnostic EM rotation which focused on interpretation of tumors, infectious and storage diseases. They also organized 100 Diagnostic EM teaching sets for Pathology residents and other URMC departmental resident trainees. Ms. Bentley has been innovative in the development of EM customized techniques to support both EM based clinical and research projects. At present, she is the Director of the Medical Center's Electron Microscope Shared Resource Facility.

Research

Ms. (de Mesy) Bentley is currently, the Director of the Electron Microscopy Shared Resource and has 29 years of teaching and diagnostic experience in Ultrastructural Pathology, Immunoelectron Microscopy labeling using gold-tagged secondary antibodies, Hematology, Dental Research, Infectious Agents, Biofilm Formation, Nanoparticle Characterization, Morphometric Analysis and X-ray Dispersive Spectroscopy using both Transmission and Scanning Electron Microsocopes. In 2011, Ms. Bentley was recruited by Dr. Edward Schwarz, the Director of the Center for Musculoskeletal Research in Orthopaedics, to investigate and analyze a mouse model of Staphylococcus aureus (MRSA and MSSA strains) infected implants and biofilm formation using scanning electron microscopy (SEM) which resulted in two Orthopaedic Research Society journal covers. In 2014, she initiated transmission electron microscopy (TEM) studies on the infected bone which had surrounded the biofilm laden implants. Ms. Bentley's high magnification TEM study led to the discovery of S. aureus' deforming into submicron rod-shaped bacteria to colonize the ostetocytic-lacunar canaliculi network of bone. This was the first evidence of S. aureus, a round non-motile 1-1.5 micron sized bacteria, becoming rod-shaped to enter (using asymmetric binary fission) the narrow confines of (0.4-0.2 micron diameter) canaliculi within live bone. This finding was published in the Journal of Bone and Mineral Research in 2016 and an electron micrograph from her study was also chosen for a book cover, Osteoimmunology, 2nd Ed, 2016. She has also authored and co-authored over 50 research publications resulting from her years of collaborative TEM and SEM imaging support to researchers here at the University. URMC Researchers who require high magnification imaging support can request a consultation and an estimate of cost prior to starting a research project. This facility provides full wet-lab services for tissue/cells processing, embedding, sectioning and utilizes a Hitachi 7650 TEM/STEM electron microscope with an attached 11 megapixel Gatan digital camera system. It is located in the basement of the Medical School building. A Zeiss Auriga scanning electron microscope housed in Wilmot Hall on the River Campus is used by the laboratory when performing SEM studies.

Credentials

Faculty Appointments

Education

1982
BS | University of Rochester
Biology

1984
MS | Univ Rochester Sch Med/Dent
Anatomy

Awards

2017 - 2017
Best Poster Teaser Presentation
Sponsor: Orthopaedic Research Society
Location: San Diego, California

1988
Current Concepts in Ultrastructural Pathology
Sponsor: Ultrapath IV
Location: Ullensvang, Norway

1984
M.S. Anatomy/Pathology

1982
B.S. Cum laude
Location: University of Rochester

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Publications

Journal Articles

4/6/2017
Shi M, de Mesy Bentley KL, Palui G, Mattoussi H, Elder A, Yang H. "The roles of surface chemistry, dissolution rate, and delivered dose in the cytotoxicity of copper nanoparticles." Nanoscale.. 2017 Apr 6; 9(14):4739-4750.

3/28/2017
Halim D, Wilson MP, Oliver D, Brosens E, Verheij JB, Han Y, Nanda V, Lyu Q, Doukas M, Stoop H, Brouwer RW, van IJcken WF, Slivano OJ, Burns AJ, Christie CK, de Mesy Bentley KL, Brooks AS, Tibboel D, Xu S, Jin ZG, Djuwantono T, Yan W, Alves MM, Hofstra RM, Miano JM. "Loss of LMOD1 impairs smooth muscle cytocontractility and causes megacystis microcolon intestinal hypoperistalsis syndrome in humans and mice." Proceedings of the National Academy of Sciences of the United States of America.. 2017 Mar 28; 114(13):E2739-E2747. Epub 2017 Mar 14.

3/15/2017
Carli AV, Bhimani S, Yang X, Shirley MB, de Mesy Bentley KL, Ross FP, Bostrom MP. "Quantification of Peri-Implant Bacterial Load and in Vivo Biofilm Formation in an Innovative, Clinically Representative Mouse Model of Periprosthetic Joint Infection." The Journal of bone and joint surgery. American volume.. 2017 Mar 15; 99(6):e25.

Books & Chapters

2016
Chapter Title: The Role of the Immune System and Bone Cells in Acute and Chronic Osteomyelitis.
Book Title: Osteoimmunology: Interactions of the Immune and Skeletal Systems
Author List: Nishitani K, Bello-Irizarry SN, de Mesy Bentley KL, Daiss JL, Schwarz EM:
Edited By: Lorenzo J, Horowitz M, Choi Y, Takayanagi H, Schett G
Published By: Elsevier2016 in London

2016
Chapter Title: Implant-Associated Biofilm
Book Title: Principles of Orthopedic Infection Management
Author List: Kohei Nishitani, Karen de Mesy Bentley, John L. Daiss
Edited By: Stephen L. Kates and Olivier Borens
Published By: George Thieme Verlag2016 in Stuttgart, Germany

1994
Chapter Title: Prostatic and urethral endocrine-paracrine cells in health and disease. An ultrastructural review.
Book Title: Ultrastructure of the Male Urogenital Glands: Prostate, Seminal Vesicles, etc
Author List: de Mesy Jensen, K.L. and di Sant'Agnese, P.A.:
Edited By: Riva, A; Riva, FT; Motta, P:
Published By: Kluwer Academic Publishers1994 in Dordrecht

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