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Karen L. Bentley, M.S.

Contact Information

Phone Numbers

Office: (585) 275-1954

Research Labs

The purpose of the URMC Electron Microscope Research Core Facility is to provide up-to-date and cost-effective support to researchers whose studies involve the use of electron microscopy.

Visit The purpose of the URMC Electron Microscope Research Core Facility is to provide up-to-date and cost-effective support to researchers whose studies involve the use of electron microscopy. Lab Website

Faculty Appointments

Biography

Professional Background

Ms.(de Mesy) Bentley has over 30 years of experience in Ultrastructural Pathology and is considered an expert in both diagnostic and research electron microscopy. She previously served as the Associate Director of the Diagnostic Electron Microscope (EM) Laboratory with P. Anthony di Sant'Agnese, M.D., a surgical pathologist, who was the Laboratory Director. Together they developed a month long resident Diagnostic EM rotation which focused on interpretation of tumors, infectious and storage diseases. They also organized 100 Diagnostic EM teaching sets for Pathology residents and other URMC departmental resident trainees. Ms. Bentley has been innovative in the development of EM customized techniques to support both EM based clinical and research projects. At present, she serves as the Director of the Medical Center's Electron Microscope Shared Resource Laboratory (EM-SRL) and supports URMC researchers in projects requiring Transmission Electron Microscopy and Scanning Electron Microscopy. In 2020 the EM-SRL acquired a Thermo Fisher Talos 120C Cryo Electron Microscope for structural biology researchers who request imaging of macromolecular structures.

Research

Ms. (de Mesy) Bentley is currently, the Director of the Electron Microscopy Shared Resource and has over 30 years of teaching and Electron Microscopy based diagnostic experience in Ultrastructural Pathology, Immunoelectron Microscopy labeling using gold-tagged secondary antibodies, Hematology, Dental Research, Infectious Agents, Biofilm Formation, Nanoparticle Characterization, Morphometric Analysis and X-ray Dispersive Spectroscopy using both Transmission and Scanning Electron Microscopes.

Ms. Bentley has published in the Journal of Orthopaedic Research in 2021, the first 3-dimensional volume reconstruction (cover of journal) of 264 consecutive 60 nm epoxy sections of Stapylococcus aureus invading the Osteocyte-Lacuno Canalicular Network (OLCN). This 3D rendering allowed us to assess that 70% of the OLCN were invaded by S. aureus, while the remaining 30% non-infected canaliculi appeared to be resistant to infection. This observation has led us to hypothesize that viable osteocytes of the OLCN can respond and resist infection, opening future research directions to explain the paradox of adjacent uninfected canaliculi and life long deep bone infection in patients with chronic osteomyelitis. This work was done in collaboration with Dr. Edward Schwarz and Dr. Jonathan Leckenby.

Previously in 2011, Ms. Bentley had been recruited by Dr. Edward Schwarz, the Director of the Center for Musculoskeletal Research in Orthopaedics, to investigate and analyze a mouse model of Staphylococcus aureus (MRSA and MSSA strains) infected implants and biofilm formation using scanning electron microscopy (SEM) that resulted in two Journal of Orthopaedic Research covers. In 2014, Ms. Bentley initiated the first transmission electron microscopy (TEM) studies on infected mouse bone which had surrounded the biofilm laden implants. Ms. Bentley's high magnification TEM study led to the discovery of S. aureus' deforming into submicron rod-shaped bacteria to colonize the OLCN of bone. This revealed for the first time how S. aureus, normally a round non-motile 1-1.5 micron sized bacteria, becomes rod-shaped to enter (using asymmetric binary fission) the submicron (0.4-0.2 micron diameter) canaliculi within live bone. This finding was published in the Journal of Bone and Mineral Research in May, 2017 and in 2016 an electron micrograph from her study was also chosen for a book cover, Osteoimmunology, 2nd Ed.

Ms Bentley has also authored and co-authored over 80 research publications resulting from her years of collaborative TEM and SEM imaging support to researchers here at the University. URMC Researchers who require high magnification imaging support can request a consultation and an estimate of cost prior to starting a research project. This facility provides full wet-lab services for tissue/cells processing, embedding, sectioning and utilizes a Hitachi 7650 TEM/STEM electron microscope with an attached 11 megapixel Gatan digital camera system. It is located in the basement of the Medical School building. A Zeiss Auriga scanning electron microscope housed in Wilmot Hall on the River Campus is used by the laboratory when performing SEM studies.

Credentials

Education

1982
BS | University of Rochester
Biology

1984
MS | Univ Rochester Sch Med/Dent
Anatomy

Awards

2017 - 2017
Best Poster Teaser Platform Presentation
Sponsor: Orthopaedic Research Society
Location: San Diego, California

1988
Current Concepts in Ultrastructural Pathology
Sponsor: Ultrapath IV
Location: Ullensvang, Norway

1984
M.S. Anatomy/Pathology

1982
B.S. Cum laude
Location: University of Rochester

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Publications

Journal Articles

6/17/2021
Adjei-Sowah E, Peng Y, Weeks J, Jonason JH, de Mesy Bentley KL, Masters E, Morita Y, Muthukrishnan G, Cherian P, Hu XE, McKenna CE, Ebetino FH, Sun S, Schwarz EM, Xie C. "Development of Bisphosphonate-Conjugated Antibiotics to Overcome Pharmacodynamic Limitations of Local Therapy: Initial Results with Carbamate Linked Sitafloxacin and Tedizolid." Antibiotics.. 2021 Jun 17; 10(6)Epub 2021 Jun 17.

2021
Muthukrishnan G, Wallimann A, Rangel-Moreno J, Bentley KLM, Hildebrand M, Mys K, Kenney HM, Sumrall ET, Daiss JL, Zeiter S, Richards RG, Schwarz EM, Moriarty TF. "Humanized Mice Exhibit Exacerbated Abscess Formation and Osteolysis During the Establishment of Implant-Associated Osteomyelitis." Frontiers in immunology.. 2021 12:651515. Epub 2021 Mar 18.

2021
Masters EA, Muthukrishnan G, Ho L, Gill AL, de Mesy Bentley KL, Galloway CA, McGrath JL, Awad HA, Gill SR, Schwarz EM. "Cell Wall Biosynthesis Modulates Bone Invasion and Osteomyelitis Pathogenesis." Frontiers in microbiology.. 2021 12:723498. Epub 2021 Aug 16.

Books & Chapters

2016
Chapter Title: The Role of the Immune System and Bone Cells in Acute and Chronic Osteomyelitis.
Book Title: Osteoimmunology: Interactions of the Immune and Skeletal Systems
Author List: Kohei Nishitani, Sheila N. Bello-Irizarry, Karen L. de Mesy Bentley, John L. Daiss, Edward M. Schwarz:
Edited By: Lorenzo J, Horowitz M, Choi Y, Takayanagi H, Schett G
Published By: Elsevier 2016 in London

2016
Chapter Title: Implant-Associated Biofilm
Book Title: Principles of Orthopedic Infection Management
Author List: Kohei Nishitani, Karen de Mesy Bentley, John L. Daiss
Edited By: Stephen L. Kates and Olivier Borens
Published By: George Thieme Verlag 2016 in Stuttgart, Germany

1994
Chapter Title: Prostatic and urethral endocrine-paracrine cells in health and disease. An ultrastructural review.
Book Title: Ultrastructure of the Male Urogenital Glands: Prostate, Seminal Vesicles, etc
Author List: Karen L. de Mesy Jensen (now Bentley) and P. Anthony di Sant'Agnese
Edited By: Riva, A; Riva, FT; Motta, P:
Published By: Kluwer Academic Publishers 1994 in Dordrecht

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