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Paul Kingsley, Ph.D.

Contact Information

Phone Numbers

Office: (585) 275-5073

Faculty Appointments

Biography

Research

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Credentials

Education

1982
BA | Reed College
Biology

1987
MS | University of Rochester
Developmental Biology

1992
PhD | University of Rochester
Developmental Biology

Post-doctoral Training & Residency

1992 - 1994
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY

Publications

Journal Articles

8/17/2021
Zhang Y, McGrath KE, Ayoub E, Kingsley PD, Yu H, Fegan K, McGlynn KA, Rudzinskas S, Palis J, Perkins AS. "Mds1, an inducible Cre allele specific to adult-repopulating hematopoietic stem cells." Cell reports.. 2021 Aug 17; 36(7):109562.

7/30/2021
Jeffery NN, Davidson C, Peslak SA, Kingsley PD, Nakamura Y, Palis J, Bulger M. "Histone H2A.X phosphorylation and Caspase-Initiated Chromatin Condensation in late-stage erythropoiesis." Epigenetics & chromatin. 2021 Jul 30; 14(1):37. Epub 2021 Jul 30.

6/21/2021
Saunders J, Niswander LM, McGrath KE, Koniski A, Catherman SC, Ture SK, Medhora M, Kingsley PD, Calvi LM, Williams JP, Morrell CN, Palis J. "Long-acting PGE2 and Lisinopril Mitigate H-ARS." Radiation research.. 2021 Jun 21; Epub 2021 Jun 21.

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