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Paul Kingsley, Ph.D.

Contact Information

Phone Numbers

Office: (585) 275-5073

Faculty Appointments

Biography

Research

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Credentials

Education

1982
BA | Reed College
Biology

1987
MS | University of Rochester
Developmental Biology

1992
PhD | University of Rochester
Developmental Biology

Post-doctoral Training & Residency

1992 - 1994
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY

Publications

Journal Articles

1/8/2019
Stolla MC, Catherman SC, Kingsley PD, Rowe RG, Koniski AD, Fegan K, Vit L, McGrath KE, Daley GQ, Palis J. "regulates age-dependent differences in murine platelet function." Blood advances.. 2019 Jan 8; 3(1):72-82.

10/26/2018
Yien YY, Shi J, Chen C, Cheung JT, Grillo AS, Shrestha R, Li L, Zhang X, Kafina MD, Kingsley PD, King MJ, Ablain J, Li H, Zon L, Palis J, Burke MD, Bauer DE, Orkin SH, Koehler CM, Phillips JD, Kaplan J, Ward DM, Lodish HF, Paw BH. "FAM210B is an erythropoietin target and regulates erythroid heme synthesis by controlling mitochondrial iron import and ferrochelatase activity." The Journal of biological chemistry.. 2018 Oct 26; Epub 2018 Oct 26.

9/2017
Zhou S, Huang YS, Kingsley PD, Cyr KH, Palis J, Wan J. "Microfluidic assay of the deformability of primitive erythroblasts." Biomicrofluidics. 2017 Sep 0; 11(5):054112. Epub 2017 Oct 23.

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