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Paul Kingsley, Ph.D.

Contact Information

Phone Numbers

Office: (585) 275-5073

Faculty Appointments

Biography

Research

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Credentials

Education

1982
BA | Reed College
Biology

1987
MS | University of Rochester
Developmental Biology

1992
PhD | University of Rochester
Developmental Biology

Post-doctoral Training & Residency

1992 - 1994
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY

Publications

Journal Articles

9/11/2020
Fox S, Myers JA, Davidson C, Getman M, Kingsley PD, Frankiewicz N, Bulger M. "Hyperacetylated chromatin domains mark cell type-specific genes and suggest distinct modes of enhancer function." Nature communications.. 2020 Sep 11; 11(1):4544. Epub 2020 Sep 11.

4/20/2020
Dege C, Fegan KH, Creamer JP, Berrien-Elliott MM, Luff SA, Kim D, Wagner JA, Kingsley PD, McGrath KE, Fehniger TA, Palis J, Sturgeon CM. "Potently Cytotoxic Natural Killer Cells Initially Emerge from Erythro-Myeloid Progenitors during Mammalian Development." Developmental cell.. 2020 Apr 20; 53(2):229-239.e7. Epub 2020 Mar 19.

7/30/2019
Calvi LM, Frisch BJ, Kingsley PD, Koniski AD, Love TM, Williams JP, Palis J. "Acute and late effects of combined internal and external radiation exposures on the hematopoietic system." International journal of radiation biology.. 2019 Jul 30; :1-15. Epub 2019 Jul 30.

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