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Paul Kingsley, Ph.D.

Contact Information

Phone Numbers

Office: (585) 275-5073



Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.



BA | Reed College

MS | University of Rochester
Developmental Biology

PhD | University of Rochester
Developmental Biology

Post-doctoral Training & Residency

1992 - 1994
Cancer Center Fellowship in Hematopoiesis, University of Rochester School of Medicine and Dentistry, Rochester, NY


Journal Articles

Nemkov T, Kingsley PD, Dzieciatkowska M, Malik J, McGrath K, Hansen KC, D'Alessandro A, Palis J. "Circulating primitive murine erythroblasts undergo complex proteomic and metabolomic changes during terminal maturation." Blood advances.. 2022 Feb 9; Epub 2022 Feb 09.

Zhang Y, McGrath KE, Ayoub E, Kingsley PD, Yu H, Fegan K, McGlynn KA, Rudzinskas S, Palis J, Perkins AS. "Mds1, an inducible Cre allele specific to adult-repopulating hematopoietic stem cells." Cell reports.. 2021 Aug 17; 36(7):109562.

Jeffery NN, Davidson C, Peslak SA, Kingsley PD, Nakamura Y, Palis J, Bulger M. "Histone H2A.X phosphorylation and Caspase-Initiated Chromatin Condensation in late-stage erythropoiesis." Epigenetics & chromatin.. 2021 Jul 30; 14(1):37. Epub 2021 Jul 30.