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Paul Kingsley, Ph.D.

Contact Information

Phone Numbers

Office: (585) 275-5073

Biography

Research

Dr. Kingsley's research interests are:
1) Embryonic Hematopoiesis
2) Erythroid Differentiation
3) Ontogeny of Hematopoietic Progenitors
4) Erythroid Precursor Self Renewal

Research in his laboratory utilizes a variety of approaches to investigate embryonic hematopoiesis. Formation of blood is a critical early event for mammalian embryos, initially occurring in the extraembryonic yolk sac of the gastrulating embryo, then migrating to the fetal liver, and finally homing to the bone marrow at the end of gestation. They are using subtractive hybridization and degenerate PCR to identify gene family members involved in specification, expansion, mobilization and migration of hematopoietic stem cells and progenitors.

An alternate approach they use is genechip technology (Affymetrix), where tens of thousands of gene products are evaluated quantitatively for expression in different regions of the developing embryo. Target tissues include microdissected regions from early murine embryos as well as embryonic cells triple-sorted for known hematopoietic/angiogenic surface markers. The spatial and temporal expression patterns of candidate genes are confirmed by in situ hybridization to both whole embryos and sections of embryonic tissues. The function of candidate molecules are tested using a variety of in vitro and in vivo model systems.

Credentials

Faculty Appointments

Education

1982
BA | Reed College
Biology

1992
PhD | University of Rochester
Developmental Biology

1987
MS | University of Rochester
Developmental Biology

Publications

Journal Articles

7/12/2017
Huang YS, Delgadillo LF, Cyr KH, Kingsley PD, An X, McGrath KE, Mohandas N, Conboy JG, Waugh RE, Wan J, Palis J. "Circulating primitive erythroblasts establish a functional, protein 4.1R-dependent cytoskeletal network prior to enucleating." Scientific reports.. 2017 Jul 12; 7(1):5164. Epub 2017 Jul 12.

10/2016
Liddicoat BJ, Hartner JC, Piskol R, Ramaswami G, Chalk AM, Kingsley PD, Sankaran VG, Wall M, Purton LE, Seeburg PH, Palis J, Orkin SH, Lu J, Li JB, Walkley CR. "Adenosine-to-inosine RNA editing by ADAR1 is essential for normal murine erythropoiesis." Experimental hematology.. 2016 Oct 0; 44(10):947-63. Epub 2016 Jul 01.

6/30/2015
McGrath KE, Frame JM, Fegan KH, Bowen JR, Conway SJ, Catherman SC, Kingsley PD, Koniski AD, Palis J. "Distinct Sources of Hematopoietic Progenitors Emerge before HSCs and Provide Functional Blood Cells in the Mammalian Embryo." Cell reports. 2015 Jun 30; 11(12):1892-904. Epub 2015 Jun 18.

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