Daniel H. Ryan, M.D.

Daniel H. Ryan, M.D.

Contact Information

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 626
Rochester, NY 14642

Office: (585) 275-3184
Fax: (585) 276-2802

Research Bio

We are interested in the molecular events determining B lymphoid lineage commitment and early differentiation of B cell progenitors. Insight into the regulation of B cell development can be applied to studies of childhood lymphoblastic leukemia, a neoplasm derived from an early B lymphoid progenitor which cannot properly commit and/or differentiate into a mature B cell.

Signals for human B cell development are incompletely understood, largely due to difficulties in culturing normal human B cell progenitors. Using a bone marrow stromal dependent culture system, we have identified colony forming human lymphoid progenitors in a very early bone marrow population (CD34+/CD19-/IL-7 receptor -) which expresses early myeloid as well as lymphoid markers, and may therefore represent cells engaged in the earliest steps of commitment to two of the major hematopoietic lineages, lymphoid and myeloid. This experimental system can be used to define critical molecular events determining the commitment of early human hematopoietic progenitors to B lymphoid differentiation.

The goal of my research is to characterize the signals for lineage commitment and B lineage differentiation of these early human lymphoid progenitors. Efforts focus on analysis of immunoglobulin gene rearrangements of individual progenitors as an indicator of early B lineage commitment using advanced cell sorting and micromanipulation techniques, and transfer of B lineage specific regulatory genes directly into normal human hematopoietic progenitors to assess their contribution to B lineage commitment and development.

Awards & Honors (Local)

My Fifth Award University of Rochester Medical Center 2000
My Fourth Award University of Rochester Medical Center 1999
My Third Award University of Rochester Medical Center 1998

Recent Journal Articles

Showing the 5 most recent journal articles. 61 available »

2016 Jul
Corsetti JP, Salzman P, Ryan D, Moss AJ, Zareba W, Sparks CE. "Influences on plasminogen activator inhibitor-2 polymorphism-associated recurrent cardiovascular disease risk in patients with high HDL cholesterol and inflammation." Atherosclerosis. 2016 Jul; 250:1-8. Epub 2016 Apr 21.
2016 Apr
Chen L, Oleksyn D, Pulvino M, Sanz I, Ryan D, Ryan C, Lin CS, Poligone B, Pentland AP, Ritchlin C, Zhao J. "A critical role for the protein kinase PKK in the maintenance of recirculating mature B cells and the development of B1 cells." Immunology letters. 2016 Apr; 172:67-78. Epub 2016 Feb 26.
2014 Apr
Moss AJ, Ryan DH, Yeaney GA. "Ganglionitis and genetic cardiac arrhythmias: more questions than answers." Circulation. Arrhythmia and electrophysiology. 2014 Apr; 7(2):190-1.
"Senior academic physicians and retirement considerations." Progress in cardiovascular diseases. 2013 55(6):611-5. Epub 2013 Mar 21.
Corsetti JP, Salzman P, Ryan D, Moss AJ, Zareba W, Sparks CE. "Plasminogen activator inhibitor-2 polymorphism associates with recurrent coronary event risk in patients with high HDL and C-reactive protein levels." PloS one. 2013 8(7):e68920. Epub 2013 Jul 09.

Current Appointments

Professor Emeritus - Department of Pathology and Laboratory Medicine (SMD) - Primary


Clinical Pathology/Laboratory Medicine - American Board of Pathology


MD | Medicine | Johns Hopkins U Sch Medicine1975
BS | Pre-Medical | University of Notre Dame1971

Post-Doctoral Training & Residency

Fellowship in Pathology at University of Rochester Medical Center12/01/1975 - 06/01/1981
Residency in Pediatrics at Univ. of Rochester School of Medicine and Dentistry06/01/1975 - 12/01/1975