Keith Nehrke, Ph.D.

Contact Information

Phone Numbers

Administrative: (585) 275-1554

Office: (585) 275-7020

Fax: (585) 442-9201

Research Labs

Our focus is on integrative physiology, and in particular on understanding how membrane electrolyte flux and pH regulation influences viability and behavior in the genetic model organism C. elegans.

Lab: (585) 275-1625

Visit Lab Website

Locations

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 675
Rochester, NY 14642

Faculty Appointments

Professor - Department of Medicine , Nephrology (SMD)
Professor - Department of Pharmacology and Physiology (SMD) - Joint

Biography

Research

The focus of my laboratory is on integrative physiology, and in particular on understanding how membrane electrolyte flux and pH regulation influences viability and behavior in the genetic model organism C. elegans. Our approach has been to exploit novel, fluorescent biosensors for measuring physiologic outputs such as calcium, pH, and redox status in unrestrained worms. Our goal is to develop a deeper understanding of how these cell physiologic processes are integrated to mediate the environmental sensitivity and functional output exhibited by live animals. We believe that this combination of physiology and genetics will allow us to discover unique mechanisms whereby cells communicate information in their native context. My background in mammalian physiology and previous work creating mouse transgenics and gene ablations has helped to allow us translate our findings in worms, as has the congenial atmosphere at the University of Rochester which promotes collaborative efforts. Our lab's funding is currently from the NSF to study calcium and pH synergism during a rhythmic behavior and from the NIH, with co-PI Dr. Paul Brookes, to study ischemic preconditioning in worms. These interactions with other faculty are one of the benefits of working in a multidisciplinary area such as Medicine.

Credentials

Education

1989
BS | St Univ at Binghamton
Biochemistry

1992
MS | Univ Rochester Sch Med/Dent
Biochemistry

1994
PhD | Univ Rochester Sch Med/Dent
Biochemistry

Post-doctoral Training & Residency

1994 - 1997
Center for Oral Biology, University of Rochester Medical Center under Dr. Larry Tabak

Publications

Journal Articles

12/1/2016
Nehrke K. "H(OH), H(OH), H(OH): a holiday perspective. Focus on "Mouse Slc4a11 expressed in Xenopus oocytes is an ideally selective H+/OH- conductance pathway that is stimulated by rises in intracellular and extracellular pH"." American journal of physiology. Cell physiology.. 2016 Dec 1; 311(6):C942-C944. Epub 2016 Oct 26.

9/16/2016
Nadtochiy SM, Schafer X, Fu D, Nehrke K, Munger J, Brookes PS. "Acidic pH Is a Metabolic Switch for 2-Hydroxyglutarate Generation and Signaling." The Journal of biological chemistry.. 2016 Sep 16; 291(38):20188-97. Epub 2016 Aug 10.

7/21/2016
Wojtovich AP, Wei AY, Sherman TA, Foster TH, Nehrke K. "Chromophore-Assisted Light Inactivation of Mitochondrial Electron Transport Chain Complex II in Caenorhabditis elegans." Scientific reports.. 2016 Jul 21; 6:29695. Epub 2016 Jul 21.

Books & Chapters

2006
Book Title: Intracellular pH measurements in vivo using green fluorescent protein variants.
Author List: Nehrke K.
Edited By: K. Strange
Published By: Methods Mol Biol. 2006

2002
Chapter Title: Ca2+-Activated Cl- Currents in Salivary and Lacrimal Glands.
Book Title: Current Topics in Membranes
Author List: Melvin J.E., Arreola J., Nehrke K., and Begenisich, T.
Edited By: C.;M. Fuller
Published By: Academic Press 2002 in San Diego

1997
Chapter Title: Experimental Approaches to Studying O-Glycosylation In Vivo.
Book Title: Techniques in Glycobiology
Author List: Nehrke K., Hagen F.H., and Tabak, L.A.
Edited By: R. Townsend and A. Hotchkiss
Published By: Marcel Dekker 1997 in New York

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