Chad Galloway is currently the Technical Manager of the Electron Microscopy Shared Resource Lab (EM-SRL). In his over 10 years of academic research experience at the University his research has encompassed diverse subject matter with a central focus of metabolic disease. Dr. Galloway brings to the EM-SRL expertise in the description and analysis of mitochondrial morphology and its reciprocal relationship with cellular bioenergetics, including the ultrastructural changes in mitochondria often perturbed by metabolic disease. During training in the Departments of Biochemistry, Anesthesiology and Pharmacology and Physiology at the University of Rochester he developed a broad background in biochemistry and cellular/molecular biology with a prevailing theme of metabolic disease including, diabetes, non-alcoholic fatty liver disease (NAFLD) and cardiovascular disease. Studies of the pathophysiology of these diseases lead to extensive work describing the relationship between mitochondrial morphology and reactive oxygen species production and the resultant oxidative injury in metabolic flux relating pathologies, observed by microscopy, to biochemical endpoints. His studies have utilized immortal and primary cell culture systems for mechanistic dissection of these processes with transition to animal models, including transgenic mouse models, to study systemic effects with a focus on cardiac and hepatic tissues. In the Department of Ophthalmology Dr. Galloway continued his utilization of microscopy, both confocal and transmission electron microscopy (TEM), as a powerful tool while dissecting the pathophysiological changes in human induced pluripotent stem cells (hiPSC) from patients with inherited macular degenerative diseases. In a first of its kind study, "Drusen in patient-derived hiPSC-RPE models of macular dystrophies" in PNAS, hiPSC retinal pigment epithelial cells were reported to autonomously recapitulate macular degenerative patient phenotypes, including drusen-like deposits analyzed by TEM and confocal microscopy, in a cell culture system which produced a potent tool for the interrogation of the underlying disease mechanisms.
Working in the EM-SRL his focus will be relating the structural changes, from the comprehensive perspective of light and fluorescence microscopy to the finite details of subcellular ultrastructure utilizing scanning and transmission electron microscopy (SEM & TEM), to their functional consequences in the pathophysiology of human disease and models thereof. In the EM-SRL Dr. Galloway works with individual users to streamline and optimize sample procurement and processing utilizing his basic science research experience to develop novel methodologies and visualize the proverbial "needle in the haystack" ultrastructural changes and organelles by electron microscopy. In this guise, he also works to advance the technology and instrumentation available for investigators in the EM-SRL while continuing basic research interests in human Staphylococcus aureus infected bone TEM studies in collaboration with Dr. Irvin Oh in the Department of Orthopedics.