Research Bio
Our long-range goal is to elucidate the underlying biological principles and functional roles of posttranscriptional RNA modifications. Currently, we are particularly interested in 2'-O-methylation and pseudouridylation in three different types of RNA, including the spliceosomal snRNA involved in pre-mRNA splicing, the telomerase RNA required for telomere length maintenance, and the messenger RNA that encodes a chemical "blueprint" for the production of a protein.
Our recent research has demonstrated that all these three types of RNA can be modified in vivo, either naturally or artificially through the RNA-guided RNA modification mechanisms. Importantly, our experimental data indicate that the posttranscriptional nucleotide modifications contribute significantly to RNA functions. We are currently carrying out three projects, aiming to address some of the fundamental questions regarding RNA modifications.
Targeted Pre-mRNA/mRNA Modification and Gene Regulation
United States Serial NO.: 11/606,995
Filed Date: December 1, 2006
Title: Nonsense Suppression and Genetic Codon Alteration by Targeted Modification
Invented by: Yi-Tao Yu, Xinliang Zhao
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Targeted Pre-mRNA/mRNA Modification and Gene Regulation
United States Serial NO.: 14/056,309
Filed Date: October 17, 2013
Title: Targeted Pre-mRNA/mRNA Modification and Gene Regulation
Invented by: Yi-Tao Yu, Xinliang Zhao
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RNA Modification to Modulate Telomerase Activity
United States Serial NO.: 13/809,975
Filed Date: July 5, 2011
Title: Targeted 2'-O-Methylation of Telomerase Non-Coding RNA
Invented by: Yi-Tao Yu, Chao Huang
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2016 Aug
Wu G, Radwan MK, Xiao M, Adachi H, Fan J, Yu YT. "The TOR signaling pathway regulates starvation-induced pseudouridylation of yeast U2 snRNA." RNA. 2016 Aug; 22(8):1146-52. Epub 2016 Jun 07. |
2016 Jul 1
Huang C, Karijolich J, Yu YT. "Detection and quantification of RNA 2'-O-methylation and pseudouridylation." Methods : a companion to Methods in enzymology. 2016 Jul 1; 103:68-76. Epub 2016 Feb 04. |
2016 Mar 15
Wu G, Adachi H, Ge J, Stephenson D, Query CC, Yu YT. "Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly." The EMBO journal. 2016 Mar 15; 35(6):654-67. Epub 2016 Feb 12. |
2016
Huang C, Wu G, Yu YT. "Purification and Functional Reconstitution of Box H/ACA Ribonucleoprotein Particles." Methods in molecular biology. 2016 1421:97-109. |
2015 Oct
Karijolich J, Yi C, Yu YT. "Transcriptome-wide dynamics of RNA pseudouridylation." Nature reviews. Molecular cell biology. 2015 Oct; 16(10):581-5. Epub 2015 Aug 19. |
