Research Bio
Our long-range goal is to elucidate the underlying biological principles and functional roles of posttranscriptional RNA modifications. Currently, we are particularly interested in 2'-O-methylation and pseudouridylation in three different types of RNA, including the spliceosomal snRNA involved in pre-mRNA splicing, the telomerase RNA required for telomere length maintenance, and the messenger RNA that encodes a chemical "blueprint" for the production of a protein.
Our recent research has demonstrated that all these three types of RNA can be modified in vivo, either naturally or artificially through the RNA-guided RNA modification mechanisms. Importantly, our experimental data indicate that the posttranscriptional nucleotide modifications contribute significantly to RNA functions. We are currently carrying out three projects, aiming to address some of the fundamental questions regarding RNA modifications.
2014
Adachi H, Yu YT. "Purification of radiolabeled RNA products using denaturing gel electrophoresis." Current protocols in molecular biology. 2014 105:4.20.1-4.20.13. Epub 2014 Jan 06. |
2013 Aug 1
Fernández IS, Ng CL, Kelley AC, Wu G, Yu YT, Ramakrishnan V. "Unusual base pairing during the decoding of a stop codon by the ribosome." Nature. 2013 Aug 1; 500(7460):107-10. Epub 2013 Jun 30. |
2013 Apr
Ge J, Yu YT. "RNA pseudouridylation: new insights into an old modification." Trends in biochemical sciences. 2013 Apr; 38(4):210-8. Epub 2013 Feb 04. |
2013
Huang, C; Yu, YT. "Synthesis and labeling of RNA in vitro." Wiley Current Protocols in Molecular Biology. 2013; . Link |
2012
Huang, C; Wu, G; Yu, YT. "Inducing nonsense suppression by targeted pseudouridylation." Nature Protocols. 2012; 7: 789-800. Link |
