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Lisa A. DeLouise, Ph.D., M.P.D.

Contact Information

Phone Numbers

Administrative: (585) 275-1998

Office: (585) 275-1810

Fax: (585) 273-1346

Research Labs

We are bionanomaterials group that investigates novel biomedical devices for diagnostic and therapeutic applications.

Lab: (585) 273-4689

Visit Lab Website

Biography

Research

Research Interest:
The overall research area in the laboratory is the creation of Smart Bandage Biomaterial Engineering and Skin Disease. This laboratory investigates the fundamental optical, morphological, and surface chemical properties of bioengineered nanoporous silicon (PSi) in developing a platform of Smart Bandage technologies targeting biosensors for point of care diagnosis of cutaneous disease, transdermal drug delivery, and tissue engineering for wound healing. PSi is fabricated from single crystal silicon wafers using an electrochemical etch process. The pore diameter, porosity (surface area and internal void volume), bioerosion, and interferometric optical properties can be tailored over a wide range to suit the application. Ongoing projects involve developing a refractive index sensitive biosensor for detection of Candida ablicans for which we've developed methods to site direct the immobilization of phage display scFv antibody receptors. Tests are underway to evaluate detection sensitivity relative to nonspecifically immobilized whole IgG aCandida antibody receptor employing commercial antigen. Fundamental insights into the factors (pore size, steric crowding, operating frequency, device architecture, blocking agent, operation protocol, etc.) that impact detection sensitivity are being explored. Biosensor tests are typically conducted on devices attached to the silicon wafer. Recently, we developed methods to detach the sensor and mount it in a polymer support. We are conducting studies to characterize the diffusion characteristics of small molecules though hydrogel films (40 micron) cast over porous silicon sensor (4 um) by monitoring changes in the optical response that result when molecules diffuse in or out of the porous silicon layer. This work will enable us to develop models for designing transdermal drug delivery systems where the optical response can be measured, while applied to the skin, to monitor the time released delivery of therapeutics concentrated within the porous reservoir. We are interested in developing a smart bandage to deliver antifungal locally to the nail matrix where nail progenitor cells live. We are also investing the morphology and phenotype dermal human fibroblast and immortalized keratinocytes (HaCaT) cells cultured on chemically modified PSi and flat silicon wafer surfaces. The goal is systematically engineer the surface chemistry, energy and topography of biomaterial scaffolds to mimic the fetal reepithelialization process. Our hypothesis is that by controlling differential cell proliferation (keratinocyte vs. fibroblast) and cell phenotype, such as integrin and metalloproteinase expression profiles or the magnitude of actin fiber extensions, novel therapies will result that can accelerate the healing of chronic wounds (ulcers) and burns with reduced scarring. A unique aspect of this research program is the melding of cross-disciplinary skills in surface science, physical chemistry, microfluidic device engineering, optics, and biological and medical sciences. This interdisciplinary approach provides a firm basis for the investigation and fabrication of new biomedical devices, while enabling a broader perspective on quantifying biological efficacy and establishing clinical utility.

Credentials

Faculty Appointments

  • Associate Professor- Department of Biomedical Engineering (SMD)
  • Associate Professor - Department of Dermatology (SMD) - Primary

Education

1979
BS | Providence College
Chemistry

1984
PhD | Penn State University
Physical Chemistry

2001
MPD | Rochester Inst Technology
Product Development

Patents

Title: Immunomodulatory Effects of Nanoparticles on the Contact Hyper Sensitivity Response
U.S. Serial #:
Filed: Apr 27, 2016
Invented By: LisaDeLouise, SamreenJatana, BrianPalmer

Publications

Journal Articles

9/2017
Pu Q, Spooner R, DeLouise LA. "Identifying drug resistant cancer cells using microbubble well arrays." Biomedical microdevices.. 2017 Sep 0; 19(3):17.

6/21/2017
Jatana S, Palmer BC, Phelan SJ, DeLouise LA. "Immunomodulatory Effects of Nanoparticles on Skin Allergy." Scientific reports.. 2017 Jun 21; 7(1):3979. Epub 2017 Jun 21.

4/14/2017
Jatana S, Palmer BC, Phelan SJ, Gelein R, DeLouise LA. "In vivo quantification of quantum dot systemic transport in C57BL/6 hairless mice following skin application post-ultraviolet radiation." Particle and fibre toxicology. 2017 Apr 14; 14(1):12. Epub 2017 Apr 14.

Books & Chapters

2009
Chapter Title: "Breeching Epithelial Barriers - Physiochemical Factors Impacting Nanoparticle Translocation and Toxicity",
Book Title: Safety of Nanoparticles: From Manufacturing to Medical Applications
Author List: DeLouise, L.A., Mortensen, L.; Elder, A.,
Edited By: Thomas J. Webster
Published By: Springer Science, Business Media2009

2008
Chapter Title: Biodetection Using Silicon Photonic Crystal Microcavities,
Book Title: Biophotonics
Author List: PM Fauchet, BL Miller, LA DeLouise, MR Lee, and H Ouyang,
Edited By: L Pavesi and PM Fauchet
Published By: Springer2008

1994
Book Title: Surface Reactions on Semiconductors Studied by Molecular Beam Reactive
Author List: Ming L. Yu and L.A. DeLouise,
Published By: Surface Science Reports1994

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