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Mark R. Buckley, Ph.D.

Mark R. Buckley, Ph.D.

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About Me

Mark Buckley received his PhD in physics from Cornell University in 2010 and worked under Dr. Louis Soslowsky as a post-doctoral fellow at the University of Pennsylvania from 2010-2012. He joined the faculty of the Department of Biomedical Engineering at the University of Rochester in January of 201...
Mark Buckley received his PhD in physics from Cornell University in 2010 and worked under Dr. Louis Soslowsky as a post-doctoral fellow at the University of Pennsylvania from 2010-2012. He joined the faculty of the Department of Biomedical Engineering at the University of Rochester in January of 2013. He has co-authored 16 publications on diverse topics ranging from three-dimensional tracking of swimming bacteria to the mechanical properties of cartilage under shear loading. Dr. Buckley is currently interested in "viscoelastic" soft biological tissues like cartilage and tendon that exhibit both fluid- and solid-like mechanical properties. His research emphasizes finding ways to control and exploit these complex properties to diagnose damage and disease, guide rehabilitation protocols and evaluate treatment and repair strategies in these tissues.

Faculty Appointments

Associate Professor - Department of Ophthalmology (SMD) - Joint

Research

Mechanics plays a key role in the onset, progression and treatment of several diseases that affect cartilage, tendon, cornea, sclera and other connective tissues. The Buckley Lab is interested in evaluating pathology-associated mechanical changes in these tissues and using our findings to develop no...
Mechanics plays a key role in the onset, progression and treatment of several diseases that affect cartilage, tendon, cornea, sclera and other connective tissues. The Buckley Lab is interested in evaluating pathology-associated mechanical changes in these tissues and using our findings to develop novel diagnostic tools and therapeutic strategies. We characterize the viscoelastic (i.e., rate- and time-dependent) mechanical response of connective tissues in vivo and in viable explants across multiple length scales using image-guided mechanical testing methods. Our current focus is on diseases of the musculoskeletal system (e.g., osteoarthritis and tendinopathy) and diseases of the eye (e.g., keratoconus and glaucoma) with significant public health impacts.

Publications

Journal Articles

Loss of drives impairments in tendon structure and function.

Adjei-Sowah E, Lecaj E, Adhikari N, Sensini C, Nichols AEC, Buckley MR, Loiselle AE

bioRxiv : the preprint server for biology.. 2024 November 14 Epub 11/14/2024.

Investigating the Tolerance of Corneal Endothelial Cells to Surgical Fluid Pressure Using Intact Porcine Eyes.

McMullen AJ, Dantsoho ZA, Chamness S, Brunelle J, Martiz J, Khalifa YM, Buckley MR

Translational vision science & technology.. 2024 November 413 (11):27. Epub 1900 01 01.

Quantification of Cartilage Poroelastic Material Properties Via Analysis of Loading-Induced Cell Death.

Kotelsky A, Carrier JS, Buckley MR

Journal of biomechanical engineering.. 2024 August 1146 (8)Epub 1900 01 01.

The Micromechanical Environment of the Impinged Achilles Tendon.

Mora KE, Mlawer SJ, Loiselle AE, Buckley MR

Small.. 2024 July 5 :e2401015. Epub 07/05/2024.

Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing.

Ackerman JE, Muscat SN, Adjei-Sowah E, Korcari A, Nichols AEC, Buckley MR, Loiselle AE

Matrix biology : journal of the International Society for Matrix Biology.. 2024 January 125 :59-72. Epub 12/13/2023.

Murine Hind Limb Explant Model for Studying the Mechanobiology of Achilles Tendon Impingement.

Wise BC, Mora KE, Lee W, Buckley MR

Journal of visualized experiments : JoVE.. 2023 December 8 (202)Epub 12/08/2023.

Identification of Periostin as a critical niche for myofibroblast dynamics and fibrosis during tendon healing.

Ackerman JE, Adjei-Sowah E, Korcari A, Muscat SN, Nichols AEC, Buckley MR, Loiselle AE

bioRxiv : the preprint server for biology.. 2023 July 21 Epub 07/21/2023.

Increased Ca signaling through Ca 1.2 induces tendon hypertrophy with increased collagen fibrillogenesis and biomechanical properties.

Li H, Korcari A, Ciufo D, Mendias CL, Rodeo SA, Buckley MR, Loiselle AE, Pitt GS, Cao C

FASEB journal : official publication of the Federation of American Societies for Experimental Biology.. 2023 July 37 (7):e23007. Epub 1900 01 01.

Scleraxis-lineage cells are required for tendon homeostasis and their depletion induces an accelerated extracellular matrix aging phenotype.

Korcari A, Nichols AEC, Buckley MR, Loiselle AE

eLife.. 2023 January 1912 Epub 01/19/2023.

Characterization of scar tissue biomechanics during adult murine flexor tendon healing.

Korcari A, Buckley MR, Loiselle AE

Journal of the mechanical behavior of biomedical materials.. 2022 June 130 :105192. Epub 03/23/2022.

Impingement in Insertional Achilles Tendinopathy Occurs Across a Larger Range of Ankle Angles and Is Associated With Increased Tendon Thickness.

Aggouras AN, Chimenti RL, Samuel Flemister A, Ketz J, Slane LC, Buckley MR, Richards MS

Foot & ankle international.. 2022 May 43 (5):683-693. Epub 01/27/2022.

Altered TGFB1 regulated pathways promote accelerated tendon healing in the superhealer MRL/MpJ mouse.

Kallenbach JG, Freeberg MAT, Abplanalp D, Alenchery RG, Ajalik RE, Muscat S, Myers JA, Ashton JM, Loiselle A, Buckley MR, van Wijnen AJ, Awad HA

Scientific reports.. 2022 February 2212 (1):3026. Epub 02/22/2022.

Ultrasound strain mapping of the mouse Achilles tendon during passive dorsiflexion.

Mora KE, Mlawer SJ, Bae AJ, Richards MS, Loiselle AE, Buckley MR

Journal of biomechanics.. 2022 February 132 :110920. Epub 12/20/2021.

Depletion of Scleraxis-lineage cells during tendon healing transiently impairs multi-scale restoration of tendon structure during early healing.

Korcari A, Muscat S, McGinn E, Buckley MR, Loiselle AE

PloS one.. 2022 17 (10):e0274227. Epub 10/14/2022.

Scleraxis-lineage cell depletion improves tendon healing and disrupts adult tendon homeostasis.

Best KT, Korcari A, Mora KE, Nichols AE, Muscat SN, Knapp E, Buckley MR, Loiselle AE

eLife.. 2021 January 2210 Epub 01/22/2021.

Tensile mechanical changes in the Achilles tendon due to Insertional Achilles tendinopathy.

Bah I, Fernandes NRJ, Chimenti RL, Ketz J, Flemister AS, Buckley MR

Journal of the mechanical behavior of biomedical materials.. 2020 December 112 :104031. Epub 08/19/2020.

Naked mole-rats are extremely resistant to post-traumatic osteoarthritis.

Taguchi T, Kotelsky A, Takasugi M, Chang M, Ke Z, Betancourt M, Buckley MR, Zuscik M, Seluanov A, Gorbunova V

Aging cell.. 2020 November 19 (11):e13255. Epub 10/28/2020.

Individualized Characterization of the Distribution of Collagen Fibril Dispersion Using Optical Aberrations of the Cornea for Biomechanical Models.

Xu M, Ramirez-Garcia MA, Narang H, Buckley MR, Lerner AL, Yoon G

Investigative ophthalmology & visual science.. 2020 August 361 (10):54. Epub 1900 01 01.

Tendon Biomechanics and Crimp Properties Following Fatigue Loading Are Influenced by Tendon Type and Age in Mice.

Zuskov A, Freedman BR, Gordon JA, Sarver JJ, Buckley MR, Soslowsky LJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2020 January 38 (1):36-42. Epub 07/23/2019.

Evidence that reduction in volume protects articular chondrocytes from mechanical impact.

Kotelsky A, Carrier JS, Aggouras A, Richards MS, Buckley MR

Connective tissue research.. 2020 61 (3-4):360-374. Epub 01/14/2020.

Non-Invasive Ultrasound Quantification of Scar Tissue Volume Identifies Early Functional Changes During Tendon Healing.

Ackerman JE, Studentsova V, Myers M, Buckley MR, Richards MS, Loiselle AE

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2019 November 37 (11):2476-2485. Epub 07/12/2019.

Design of a Bioreactor to Assess the Effect of Passive Joint Loading in a Live Chick Embryo .

Stein MJ, Buckley MR, Manuele D, Gutierrez A, Loor JS, Nguyen PK, Kuo CK

Tissue engineering. Part C, Methods.. 2019 November 25 (11):655-661. Epub 10/30/2019.

Real-time Visualization and Analysis of Chondrocyte Injury Due to Mechanical Loading in Fully Intact Murine Cartilage Explants.

Kotelsky A, Carrier JS, Buckley MR

Journal of visualized experiments : JoVE.. 2019 January 7 (143)Epub 01/07/2019.

Vulnerability of corneal endothelial cells to mechanical trauma from indentation forces assessed using contact mechanics and fluorescence microscopy.

Ramirez-Garcia MA, Khalifa YM, Buckley MR

Experimental eye research.. 2018 October 175 :73-82. Epub 06/05/2018.

Obesity/Type II Diabetes Promotes Function-limiting Changes in Murine Tendons that are not reversed by Restoring Normal Metabolic Function.

Studentsova V, Mora KM, Glasner MF, Buckley MR, Loiselle AE

Scientific reports.. 2018 June 158 (1):9218. Epub 06/15/2018.

Depth-Dependent Out-of-Plane Young's Modulus of the Human Cornea.

Ramirez-Garcia MA, Sloan SR, Nidenberg B, Khalifa YM, Buckley MR

Current eye research.. 2018 May 43 (5):595-604. Epub 12/28/2017.

Serpine1 Knockdown Enhances MMP Activity after Flexor Tendon Injury in Mice: Implications for Adhesions Therapy.

Freeberg MAT, Farhat YM, Easa A, Kallenbach JG, Malcolm DW, Buckley MR, Benoit DSW, Awad HA

Scientific reports.. 2018 April 118 (1):5810. Epub 04/11/2018.

Viscoelastic heating of insulated bovine intervertebral disc.

Newman HR, Bowles RD, Buckley MR

JOR spine.. 2018 March 1 (1):e1002. Epub 03/09/2018.

An Alternative Method to Characterize the Quasi-Static, Nonlinear Material Properties of Murine Articular Cartilage.

Kotelsky A, Woo CW, Delgadillo LF, Richards MS, Buckley MR

Journal of biomechanical engineering.. 2018 January 1140 (1):0110071-9. Epub 1900 01 01.

Aging does not alter tendon mechanical properties during homeostasis, but does impair flexor tendon healing.

Ackerman JE, Bah I, Jonason JH, Buckley MR, Loiselle AE

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2017 December 35 (12):2716-2724. Epub 05/03/2017.

Insertional achilles tendinopathy associated with altered transverse compressive and axial tensile strain during ankle dorsiflexion.

Chimenti RL, Bucklin M, Kelly M, Ketz J, Flemister AS, Richards MS, Buckley MR

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2017 April 35 (4):910-915. Epub 06/23/2016.

Injury response of geriatric mouse patellar tendons.

Mienaltowski MJ, Dunkman AA, Buckley MR, Beason DP, Adams SM, Birk DE, Soslowsky LJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2016 July 34 (7):1256-63. Epub 01/18/2016.

Utility of Ultrasound for Imaging Osteophytes in Patients With Insertional Achilles Tendinopathy.

Chimenti RL, Chimenti PC, Buckley MR, Houck JR, Flemister AS

Archives of physical medicine and rehabilitation.. 2016 July 97 (7):1206-9. Epub 12/29/2015.

Ultrasound strain mapping of Achilles tendon compressive strain patterns during dorsiflexion.

Chimenti RL, Flemister AS, Ketz J, Bucklin M, Buckley MR, Richards MS

Journal of biomechanics.. 2016 January 449 (1):39-44. Epub 11/30/2015.

Mechanical changes in the Achilles tendon due to insertional Achilles tendinopathy.

Bah I, Kwak ST, Chimenti RL, Richards MS, P Ketz J, Samuel Flemister A, Buckley MR

Journal of the mechanical behavior of biomedical materials.. 2016 January 53 :320-328. Epub 08/19/2015.

Evaluating changes in tendon crimp with fatigue loading as an ex vivo structural assessment of tendon damage.

Freedman BR, Zuskov A, Sarver JJ, Buckley MR, Soslowsky LJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2015 June 33 (6):904-10. Epub 04/27/2015.

Effects of enzymatic treatments on the depth-dependent viscoelastic shear properties of articular cartilage.

Griffin DJ, Vicari J, Buckley MR, Silverberg JL, Cohen I, Bonassar LJ

Journal of orthopaedic research : official publication of the Orthopaedic Research Society.. 2014 December 32 (12):1652-7. Epub 09/05/2014.

The location- and depth-dependent mechanical response of the human cornea under shear loading.

Sloan SR, Khalifa YM, Buckley MR

Investigative ophthalmology & visual science.. 2014 October 3055 (12):7919-24. Epub 10/30/2014.

Biomechanical and structural response of healing Achilles tendon to fatigue loading following acute injury.

Freedman BR, Sarver JJ, Buckley MR, Voleti PB, Soslowsky LJ

Journal of biomechanics.. 2014 June 2747 (9):2028-34. Epub 11/11/2013.

The injury response of aged tendons in the absence of biglycan and decorin.

Dunkman AA, Buckley MR, Mienaltowski MJ, Adams SM, Thomas SJ, Kumar A, Beason DP, Iozzo RV, Birk DE, Soslowsky LJ

Matrix biology : journal of the International Society for Matrix Biology.. 2014 April 35 :232-8. Epub 10/21/2013.

The tendon injury response is influenced by decorin and biglycan.

Dunkman AA, Buckley MR, Mienaltowski MJ, Adams SM, Thomas SJ, Satchell L, Kumar A, Pathmanathan L, Beason DP, Iozzo RV, Birk DE, Soslowsky LJ

Annals of biomedical engineering.. 2014 March 42 (3):619-30. Epub 09/27/2013.

The dynamics of collagen uncrimping and lateral contraction in tendon and the effect of ionic concentration.

Buckley MR, Sarver JJ, Freedman BR, Soslowsky LJ

Journal of biomechanics.. 2013 September 346 (13):2242-9. Epub 07/19/2013.

Spatial periodicity in growth plate shear mechanical properties is disrupted by vitamin D deficiency.

Sevenler D, Buckley MR, Kim G, van der Meulen MC, Cohen I, Bonassar LJ

Journal of biomechanics.. 2013 June 2146 (10):1597-603. Epub 05/21/2013.

Validation of an empirical damage model for aging and in vivo injury of the murine patellar tendon.

Buckley MR, Dunkman AA, Reuther KE, Kumar A, Pathmanathan L, Beason DP, Birk DE, Soslowsky LJ

Journal of biomechanical engineering.. 2013 April 135 (4):041005. Epub 1900 01 01.

Localization of viscous behavior and shear energy dissipation in articular cartilage under dynamic shear loading.

Buckley MR, Bonassar LJ, Cohen I

Journal of biomechanical engineering.. 2013 March 1135 (3):31002. Epub 1900 01 01.

Decorin expression is important for age-related changes in tendon structure and mechanical properties.

Dunkman AA, Buckley MR, Mienaltowski MJ, Adams SM, Thomas SJ, Satchell L, Kumar A, Pathmanathan L, Beason DP, Iozzo RV, Birk DE, Soslowsky LJ

Matrix biology : journal of the International Society for Matrix Biology.. 2013 January 32 (1):3-13. Epub 11/23/2012.

The location-specific role of proteoglycans in the flexor carpi ulnaris tendon.

Buckley MR, Huffman GR, Iozzo RV, Birk DE, Soslowsky LJ

Connective tissue research.. 2013 54 (6):367-73. Epub 10/10/2013.

Distributions of types I, II and III collagen by region in the human supraspinatus tendon.

Buckley MR, Evans EB, Matuszewski PE, Chen YL, Satchel LN, Elliott DM, Soslowsky LJ, Dodge GR

Connective tissue research.. 2013 54 (6):374-9. Epub 1900 01 01.

Implanted adipose progenitor cells as physicochemical regulators of breast cancer.

Chandler EM, Seo BR, Califano JP, Andresen Eguiluz RC, Lee JS, Yoon CJ, Tims DT, Wang JX, Cheng L, Mohanan S, Buckley MR, Cohen I, Nikitin AY, Williams RM, Gourdon D, Reinhart-King CA, Fischbach C

Proceedings of the National Academy of Sciences of the United States of America.. 2012 June 19109 (25):9786-91. Epub 06/04/2012.

Insights into interstitial flow, shear stress, and mass transport effects on ECM heterogeneity in bioreactor-cultivated engineered cartilage hydrogels.

Chen T, Buckley M, Cohen I, Bonassar L, Awad HA

Biomechanics and modeling in mechanobiology.. 2012 May 11 (5):689-702. Epub 08/19/2011.

Tendon healing: repair and regeneration.

Voleti PB, Buckley MR, Soslowsky LJ

Annual review of biomedical engineering.. 2012 14 :47-71. Epub 1900 01 01.

The effects of needle puncture injury on microscale shear strain in the intervertebral disc annulus fibrosus.

Michalek AJ, Buckley MR, Bonassar LJ, Cohen I, Iatridis JC

The spine journal : official journal of the North American Spine Society.. 2010 December 10 (12):1098-105. Epub 10/23/2010.

High-resolution spatial mapping of shear properties in cartilage.

Buckley MR, Bergou AJ, Fouchard J, Bonassar LJ, Cohen I

Journal of biomechanics.. 2010 March 343 (4):796-800. Epub 11/05/2009.

Direct measurements of island growth and step-edge barriers in colloidal epitaxy.

Ganapathy R, Buckley MR, Gerbode SJ, Cohen I

Science.. 2010 January 22327 (5964):445-8. Epub 1900 01 01.

Measurement of local strains in intervertebral disc anulus fibrosus tissue under dynamic shear: contributions of matrix fiber orientation and elastin content.

Michalek AJ, Buckley MR, Bonassar LJ, Cohen I, Iatridis JC

Journal of biomechanics.. 2009 October 1642 (14):2279-85. Epub 08/06/2009.

High resolution shear profile measurements in entangled polymers.

Hayes KA, Buckley MR, Cohen I, Archer LA

Physical review letters.. 2008 November 21101 (21):218301. Epub 11/17/2008.

Mapping the depth dependence of shear properties in articular cartilage.

Buckley MR, Gleghorn JP, Bonassar LJ, Cohen I

Journal of biomechanics.. 2008 August 741 (11):2430-7. Epub 07/10/2008.