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Mitchell R. O'Connell, Ph.D.

Mitchell R. O'Connell, Ph.D.

Research Lab

O'Connell Lab Website

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About Me

Faculty Appointments

Assistant Professor - Department of Biochemistry and Biophysics (SMD)



Journal Articles

Unveiling the RNA-mediated allosteric activation discloses functional hotspots in CRISPR-Cas13a.

Sinha S, Molina Vargas AM, Arantes PR, Patel A, O'Connell MR, Palermo G

Nucleic acids research.. 2023 November 30 Epub 11/30/2023.

New design strategies for ultra-specific CRISPR-Cas13a-based RNA detection with single-nucleotide mismatch sensitivity.

Molina Vargas AM, Sinha S, Osborn R, Arantes PR, Patel A, Dewhurst S, Hardy DJ, Cameron A, Palermo G, O'Connell MR

Nucleic acids research.. 2023 November 30 Epub 11/30/2023.

Expression and purification of the mitochondrial transmembrane protein FAM210A in Escherichia coli.

Hollinger J, Wu J, Awayda KM, O'Connell MR, Yao P

Protein expression and purification.. 2023 October 210 :106322. Epub 06/15/2023.

Aerosol Jet Printing-Enabled Dual-Function Electrochemical and Colorimetric Biosensor for SARS-CoV-2 Detection.

Liu L, Xu Z, Molina Vargas AM, Dollery SJ, Schrlau MG, Cormier D, O'Connell MR, Tobin GJ, Du K

Analytical chemistry.. 2023 July 28 Epub 07/28/2023.

New design strategies for ultra-specific CRISPR-Cas13a-based RNA-diagnostic tools with single-nucleotide mismatch sensitivity.

Vargas AMM, Osborn R, Sinha S, Arantes PR, Patel A, Dewhurst S, Palermo G, O'Connell MR

bioRxiv : the preprint server for biology.. 2023 July 28 Epub 07/28/2023.

RNA-mediated Allosteric Activation in CRISPR-Cas13a.

Sinha S, Molina Vargas AM, Arantes PR, Patel A, O'Connell MR, Palermo G

bioRxiv : the preprint server for biology.. 2023 July 27 Epub 07/27/2023.

Csx28 is a membrane pore that enhances CRISPR-Cas13b-dependent antiphage defense.

VanderWal AR, Park JU, Polevoda B, Nicosia JK, Molina Vargas AM, Kellogg EH, O'Connell MR

Science.. 2023 April 28380 (6643):410-415. Epub 04/27/2023.

Methods special issue: RNA-targeting technologies.

Knott GJ, Lapinaite A, O'Connell MR

Methods : a companion to Methods in enzymology.. 2023 April 212 :10-11. Epub 02/13/2023.

Gold Nanoparticle Enabled Localized Surface Plasmon Resonance on Unique Gold Nanomushroom Structures for On-Chip CRISPR-Cas13a Sensing.

Waitkus J, Chang Y, Liu L, Puttaswamy SV, Chung T, Vargas AMM, Dollery SJ, O'Connell MR, Cai H, Tobin GJ, Bhalla N, Du K

Advanced materials interfaces.. 2023 January 510 (1)Epub 11/27/2022.

Optimization of specific RNA knockdown in mammalian cells with CRISPR-Cas13.

Joseph Davis Burris B, Moises Molina Vargas A, Park BJ, O'Connell MR

Methods : a companion to Methods in enzymology.. 2022 August 17 Epub 08/17/2022.

Protein folding stabilities are a major determinant of oxidation rates for buried methionine residues.

Walker EJ, Bettinger JQ, Welle KA, Hryhorenko JR, Molina Vargas AM, O'Connell MR, Ghaemmaghami S

The Journal of biological chemistry.. 2022 March 25 :101872. Epub 03/25/2022.

Gold Nanoparticle-Labeled CRISPR-Cas13a Assay for the Sensitive Solid-State Nanopore Molecular Counting.

Liu L, Xu Z, Awayda K, Dollery SJ, Bao M, Fan J, Cormier D, O'Connell M, Tobin GJ, Du K

Advanced materials technologies.. 2022 March 7 (3)Epub 01/22/2022.

THUMPD1 bi-allelic variants cause loss of tRNA acetylation and a syndromic neurodevelopmental disorder.

Broly M, Polevoda BV, Awayda KM, Tong N, Lentini J, Besnard T, Deb W, O'Rourke D, Baptista J, Ellard S, Almannai M, Hashem M, Abdulwahab F, Shamseldin H, Al-Tala S, Alkuraya FS, Leon A, van Loon RLE, Ferlini A, Sanchini M, Bigoni S, Ciorba A, van Bokhoven H, Iqbal Z, Al-Maawali A, Al-Murshedi F, Ganesh A, Al-Mamari W, Lim SC, Pais LS, Brown N, Riazuddin S, Bézieau S, Fu D, Isidor B, Cogné B, O'Connell MR

American journal of human genetics.. 2022 February 11 Epub 02/11/2022.

Put on Your Para-spectacles: The Development of Optimized CRISPR-Cas13-Based Approaches to Image RNA Dynamics in Real Time.

Davis BJ, O'Connell MR

Molecular cell.. 2020 January 1677 (2):207-209. Epub 1900 01 01.

Molecular Mechanisms of RNA Targeting by Cas13-containing Type VI CRISPR-Cas Systems.

O'Connell M

Journal of molecular biology.. 2019 January 4431 (1):66-87. Epub 06/22/2018.

RNA Binding and HEPN-Nuclease Activation Are Decoupled in CRISPR-Cas13a.

Tambe A, East-Seletsky A, Knott GJ, Doudna JA, O'Connell MR

Cell reports.. 2018 July 2424 (4):1025-1036. Epub 1900 01 01.

Engineering RNA-Binding Proteins by Modular Assembly of RanBP2-Type Zinc Fingers.

De Franco S, O'Connell MR, Vandevenne M

Methods in molecular biology.. 2018 1867 :57-74. Epub 1900 01 01.

Guide-bound structures of an RNA-targeting A-cleaving CRISPR-Cas13a enzyme.

Knott GJ, East-Seletsky A, Cofsky JC, Holton JM, Charles E, O'Connell MR, Doudna JA

Nature structural & molecular biology.. 2017 October 24 (10):825-833. Epub 09/11/2017.

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes.

East-Seletsky A, O'Connell MR, Burstein D, Knott GJ, Doudna JA

Molecular cell.. 2017 May 466 (3):373-383.e3. Epub 1900 01 01.

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection.

East-Seletsky A, O'Connell MR, Knight SC, Burstein D, Cate JH, Tjian R, Doudna JA

Nature.. 2016 October 13538 (7624):270-273. Epub 09/26/2016.

Programmable RNA Tracking in Live Cells with CRISPR/Cas9.

Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, Yeo GW

Cell.. 2016 April 7165 (2):488-96. Epub 03/17/2016.

Single-Stranded DNA Cleavage by Divergent CRISPR-Cas9 Enzymes.

Ma E, Harrington LB, O'Connell MR, Zhou K, Doudna JA

Molecular cell.. 2015 November 560 (3):398-407. Epub 1900 01 01.

Programmable RNA recognition and cleavage by CRISPR/Cas9.

O'Connell MR, Oakes BL, Sternberg SH, East-Seletsky A, Kaplan M, Doudna JA

Nature.. 2014 December 11516 (7530):263-6. Epub 09/28/2014.

Engineering specificity changes on a RanBP2 zinc finger that binds single-stranded RNA.

Vandevenne M, O'Connell MR, Helder S, Shepherd NE, Matthews JM, Kwan AH, Segal DJ, Mackay JP

Angewandte Chemie.. 2014 July 2153 (30):7848-52. Epub 07/09/2014.

The identification and structure of an N-terminal PR domain show that FOG1 is a member of the PRDM family of proteins.

Clifton MK, Westman BJ, Thong SY, O'Connell MR, Webster MW, Shepherd NE, Quinlan KG, Crossley M, Blobel GA, Mackay JP

PloS one.. 2014 9 (8):e106011. Epub 08/27/2014.

A structural analysis of DNA binding by myelin transcription factor 1 double zinc fingers.

Gamsjaeger R, O'Connell MR, Cubeddu L, Shepherd NE, Lowry JA, Kwan AH, Vandevenne M, Swanton MK, Matthews JM, Mackay JP

The Journal of biological chemistry.. 2013 December 6288 (49):35180-91. Epub 10/06/2013.

A rapid method for assessing the RNA-binding potential of a protein.

Bendak K, Loughlin FE, Cheung V, O'Connell MR, Crossley M, Mackay JP

Nucleic acids research.. 2012 August 40 (14):e105. Epub 04/03/2012.

Modular assembly of RanBP2-type zinc finger domains to target single-stranded RNA.

O'Connell MR, Vandevenne M, Nguyen CD, Matthews JM, Gamsjaeger R, Segal DJ, Mackay JP

Angewandte Chemie.. 2012 May 2951 (22):5371-5. Epub 04/19/2012.

Two-timing zinc finger transcription factors liaising with RNA.

Burdach J, O'Connell MR, Mackay JP, Crossley M

Trends in biochemical sciences.. 2012 May 37 (5):199-205. Epub 03/07/2012.

Synthesis of the bacteriocin glycopeptide sublancin 168 and S-glycosylated variants.

Hsieh YS, Wilkinson BL, O'Connell MR, Mackay JP, Matthews JM, Payne RJ

Organic letters.. 2012 April 614 (7):1910-3. Epub 03/28/2012.

The structural analysis of protein-protein interactions by NMR spectroscopy.

O'Connell MR, Gamsjaeger R, Mackay JP

Proteomics.. 2009 December 9 (23):5224-32. Epub 1900 01 01.