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Mark E. Dumont, Ph.D.


Showing all 62 journal articles & 0 books available

Journal Articles

Dumont ME, Konopka JB. "Comparison of Experimental Approaches Used to Determine the Structure and Function of the Class D G Protein-Coupled Yeast ?-Factor Receptor." Biomolecules.. 2022 May 30; 12(6)Epub 2022 May 30.

Connelly SM, Sridharan R, Naider F, Dumont ME. "Oligomerization of Yeast ?-factor Receptor Detected by Fluorescent Energy Transfer Between Ligands." Biophysical journal.. 2021 Oct 7; Epub 2021 Oct 07.

Zhu H, Mathew E, Connelly SM, Zuber J, Sullivan M, Piepenbrink MS, Kobie JJ, Dumont ME. "Identification of variant HIV envelope proteins with enhanced affinities for precursors to anti-gp41 broadly neutralizing antibodies." PloS one.. 2019 14(9):e0221550. Epub 2019 Sep 10.

Mathew E, Zhu H, Connelly SM, Sullivan MA, Brewer MG, Piepenbrink MS, Kobie JJ, Dewhurst S, Dumont ME. "Display of the HIV envelope protein at the yeast cell surface for immunogen development." PloS one.. 2018 13(10):e0205756. Epub 2018 Oct 18.

Clark KM, Jenkins JL, Fedoriw N, Dumont ME. "Human CaaX protease ZMPSTE24 expressed in yeast: Structure and inhibition by HIV protease inhibitors." Protein science : a publication of the Protein Society.. 2017 Feb; 26(2):242-257. Epub 2016 Nov 11.

Coudray N, L Seyler S, Lasala R, Zhang Z, Clark KM, Dumont ME, Rohou A, Beckstein O, Stokes DL. "Structure of the SLC4 transporter Bor1p in an inward-facing conformation." Protein science : a publication of the Protein Society.. 2017 Jan; 26(1):130-145. Epub 2016 Oct 21.

Sridharan R, Connelly SM, Naider F, Dumont ME. "Variable Dependence of Signaling Output on Agonist Occupancy of Ste2p, a G Protein-coupled Receptor in Yeast." The Journal of biological chemistry.. 2016 Nov 11; 291(46):24261-24279. Epub 2016 Sep 19.

Coudray N, Lasala R, Zhang Z, Clark KM, Dumont ME, Stokes DL. "Deducing the symmetry of helical assemblies: Applications to membrane proteins." Journal of structural biology.. 2016 Aug; 195(2):167-178. Epub 2016 May 30.

Zhu G, Koszelak-Rosenblum M, Connelly SM, Dumont ME, Malkowski MG. "The Crystal Structure of an Integral Membrane Fatty Acid ?-Hydroxylase." The Journal of biological chemistry.. 2015 Dec 11; 290(50):29820-33. Epub 2015 Oct 28.

Zuber J, Danial SA, Connelly SM, Naider FR, Dumont ME. "Identification of destabilizing and stabilizing mutations of Ste2p, a G protein-coupled receptor in Saccharomyces cerevisiae." Biochemistry.. 2015 Mar 10; 54(9):1787-806. Epub 2015 Feb 24.

Mathew E, Dumont ME. "A Novel Screening Approach for Optimal and Functional Fusion of T4 Lysozyme in GPCRs." Methods in enzymology.. 2015 557:27-43. Epub 2015 Mar 24.

Cohen LS, Arshava B, Kauffman S, Mathew E, Fracchiolla KE, Ding FX, Dumont ME, Becker JM, Naider F. "Guided reconstitution of membrane protein fragments." Biopolymers.. 2014 Jan; 102(1):16-29.

Sridharan R, Zuber J, Connelly SM, Mathew E, Dumont ME. "Fluorescent approaches for understanding interactions of ligands with G protein coupled receptors." Biochimica et biophysica acta.. 2014 Jan; 1838(1 Pt A):15-33. Epub 2013 Sep 18.

Pryor EE, Horanyi PS, Clark KM, Fedoriw N, Connelly SM, Koszelak-Rosenblum M, Zhu G, Malkowski MG, Wiener MC, Dumont ME. "Structure of the integral membrane protein CAAX protease Ste24p." Science.. 2013 Mar 29; 339(6127):1600-4.

Pieper U, Schlessinger A, Kloppmann E, Chang GA, Chou JJ, Dumont ME, Fox BG, Fromme P, Hendrickson WA, Malkowski MG, Rees DC, Stokes DL, Stowell MH, Wiener MC, Rost B, Stroud RM, Stevens RC, Sali A. "Coordinating the impact of structural genomics on the human ?-helical transmembrane proteome." Nature structural & molecular biology.. 2013 Feb; 20(2):135-8.

Mathew E, Ding FX, Naider F, Dumont ME. "Functional fusions of T4 lysozyme in the third intracellular loop of a G protein-coupled receptor identified by a random screening approach in yeast." Protein engineering, design & selection : PEDS.. 2013 Jan; 26(1):59-71. Epub 2012 Oct 16.

Gehret AU, Connelly SM, Dumont ME. "Functional and physical interactions among Saccharomyces cerevisiae ?-factor receptors." Eukaryotic cell.. 2012 Oct; 11(10):1276-88. Epub 2012 Aug 24.

Lesoine JF, Venkataraman PA, Maloney PC, Dumont ME, Novotny L. "Nanochannel-based single molecule recycling." Nano letters.. 2012 Jun 13; 12(6):3273-8. Epub 2012 Jun 04.

Taslimi A, Mathew E, Celi? A, Wessel S, Dumont ME. "Identifying functionally important conformational changes in proteins: activation of the yeast ?-factor receptor Ste2p." Journal of molecular biology.. 2012 May 18; 418(5):367-78. Epub 2012 Mar 03.

Kang DC, Venkataraman PA, Dumont ME, Maloney PC. "Oligomeric state of the oxalate transporter, OxlT." Biochemistry.. 2011 Oct 4; 50(39):8445-53. Epub 2011 Sep 08.

Mathew E, Bajaj A, Connelly SM, Sargsyan H, Ding FX, Hajduczok AG, Naider F, Dumont ME. "Differential interactions of fluorescent agonists and antagonists with the yeast G protein coupled receptor Ste2p." Journal of molecular biology.. 2011 Jun 17; 409(4):513-28. Epub 2011 Apr 06.

Tantry SJ, Ding FX, Dumont ME, Becker JM, Naider FR. "Binding of fluorinated phenylalanine alpha-factor analogues to Ste2p: evidence for a cation-pi binding interaction between a peptide ligand and its cognate G protein-coupled receptor." Biochemistry.. 2010 Jun 22; 49(24):5007-15.

Clark KM, Fedoriw N, Robinson K, Connelly SM, Randles J, Malkowski MG, Detitta GT, Dumont ME. "Purification of transmembrane proteins from Saccharomyces cerevisiae for X-ray crystallography." Protein expression and purification.. 2010 Jun; 71(2):207-23. Epub 2010 Jan 04.

Shi S, Notenboom S, Dumont ME, Ballatori N. "Identification of human gene products containing Pro-Pro-x-Tyr (PY) motifs that enhance glutathione and endocytotic marker uptake in yeast." Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology.. 2010 25(2-3):293-306. Epub 2010 Jan 12.

Chloupková M, Pickert A, Lee JY, Souza S, Trinh YT, Connelly SM, Dumont ME, Dean M, Urbatsch IL. "Expression of 25 human ABC transporters in the yeast Pichia pastoris and characterization of the purified ABCC3 ATPase activity." Biochemistry.. 2007 Jul 10; 46(27):7992-8003. Epub 2007 Jun 15.

Bajaj A, Connelly SM, Gehret AU, Naider F, Dumont ME. "Role of extracellular charged amino acids in the yeast alpha-factor receptor." Biochimica et biophysica acta.. 2007 Jun; 1773(6):707-17. Epub 2007 Feb 17.

White MA, Clark KM, Grayhack EJ, Dumont ME. "Characteristics affecting expression and solubilization of yeast membrane proteins." Journal of molecular biology.. 2007 Jan 19; 365(3):621-36. Epub 2006 Oct 06.

Wang Z, Stalcup LD, Harvey BJ, Weber J, Chloupkova M, Dumont ME, Dean M, Urbatsch IL. "Purification and ATP hydrolysis of the putative cholesterol transporters ABCG5 and ABCG8." Biochemistry.. 2006 Aug 15; 45(32):9929-39.

Gehret AU, Bajaj A, Naider F, Dumont ME. "Oligomerization of the yeast alpha-factor receptor: implications for dominant negative effects of mutant receptors." The Journal of biological chemistry.. 2006 Jul 28; 281(30):20698-714. Epub 2006 May 18.

Gelperin DM, White MA, Wilkinson ML, Kon Y, Kung LA, Wise KJ, Lopez-Hoyo N, Jiang L, Piccirillo S, Yu H, Gerstein M, Dumont ME, Phizicky EM, Snyder M, Grayhack EJ. "Biochemical and genetic analysis of the yeast proteome with a movable ORF collection." Genes & development.. 2005 Dec 1; 19(23):2816-26.

Bajaj A, Celi? A, Ding FX, Naider F, Becker JM, Dumont ME. "A fluorescent alpha-factor analogue exhibits multiple steps on binding to its G protein coupled receptor in yeast." Biochemistry.. 2004 Oct 26; 43(42):13564-78.

Alexandrov A, Vignali M, LaCount DJ, Quartley E, de Vries C, De Rosa D, Babulski J, Mitchell SF, Schoenfeld LW, Fields S, Hol WG, Dumont ME, Phizicky EM, Grayhack EJ. "A facile method for high-throughput co-expression of protein pairs." Molecular & cellular proteomics : MCP.. 2004 Sep; 3(9):934-8. Epub 2004 Jul 07.

Roginskaya M, Connelly SM, Kim KS, Patel D, Dumont ME. "Effects of mutations in the N terminal region of the yeast G protein alpha-subunit Gpa1p on signaling by pheromone receptors." Molecular genetics and genomics : MGG.. 2004 Mar; 271(2):237-48. Epub 2004 Feb 07.

Celi? A, Connelly SM, Martin NP, Dumont ME. "Intensive mutational analysis of G protein-coupled receptors in yeast." Methods in molecular biology.. 2004 237:105-20.

Celi? A, Martin NP, Son CD, Becker JM, Naider F, Dumont ME. "Sequences in the intracellular loops of the yeast pheromone receptor Ste2p required for G protein activation." Biochemistry.. 2003 Mar 18; 42(10):3004-17.

Connelly, S.M.; Yuan, J.; Ding, F.X.; Naider, F.; Becker, J.M.; Dumont, M.E. ;. "Effects of Inserting Intracellular Loops of the Yeast a-factor Receptor, Ste3p, into the a-factor Receptor, Ste2p" . Yeast. 2003; : in press.

Arevalo, E.; Estephan, R.; Madeo, J.; Arshava, B.; Dumont, M.E.; Becker, J.M.; Naider, F.;. "Biosynthesis and Biophysical Analysis of Domains of a Yeast G protein-coupled receptor" . Biopolymers (Peptide Science). 2003; : in press.

Martin NP, Celi? A, Dumont ME. "Mutagenic mapping of helical structures in the transmembrane segments of the yeast alpha-factor receptor." Journal of molecular biology.. 2002 Apr 12; 317(5):765-88.

Erlenbach I, Kostenis E, Schmidt C, Serradeil-Le Gal C, Raufaste D, Dumont ME, Pausch MH, Wess J. "Single amino acid substitutions and deletions that alter the G protein coupling properties of the V2 vasopressin receptor identified in yeast by receptor random mutagenesis." The Journal of biological chemistry.. 2001 Aug 3; 276(31):29382-92. Epub 2001 May 25.

Sommers CM, Martin NP, Akal-Strader A, Becker JM, Naider F, Dumont ME. "A limited spectrum of mutations causes constitutive activation of the yeast alpha-factor receptor." Biochemistry.. 2000 Jun 13; 39(23):6898-909.

Leavitt LM, Macaluso CR, Kim KS, Martin NP, Dumont ME. "Dominant negative mutations in the alpha-factor receptor, a G protein-coupled receptor encoded by the STE2 gene of the yeast Saccharomyces cerevisiae." Molecular & general genetics : MGG.. 1999 Jul; 261(6):917-32.

Martin NP, Leavitt LM, Sommers CM, Dumont ME. "Assembly of G protein-coupled receptors from fragments: identification of functional receptors with discontinuities in each of the loops connecting transmembrane segments." Biochemistry.. 1999 Jan 12; 38(2):682-95.

Sommers, C.M.; Dumont, M.E.;. "Genetic Approaches for Studying the Structure and Function of G Protein-Coupled receptors in Yeast in Structure-Function Analysis of G Protein-Coupled Receptors" . ( J. Wess, ed.) Vol. III of Receptor Biochemistry and Methodology, , John Wiley and Sons, New York. 1999; : 141.

Rebbeor JF, Connolly GC, Dumont ME, Ballatori N. "ATP-dependent transport of reduced glutathione on YCF1, the yeast orthologue of mammalian multidrug resistance associated proteins." The Journal of biological chemistry.. 1998 Dec 11; 273(50):33449-54.

Rebbeor JF, Connolly GC, Dumont ME, Ballatori N. "ATP-dependent transport of reduced glutathione in yeast secretory vesicles." The Biochemical journal.. 1998 Sep 15; 334 ( Pt 3):723-9.

Pollock WB, Rosell FI, Twitchett MB, Dumont ME, Mauk AG. "Bacterial expression of a mitochondrial cytochrome c. Trimethylation of lys72 in yeast iso-1-cytochrome c and the alkaline conformational transition." Biochemistry.. 1998 Apr 28; 37(17):6124-31.

Sommers CM, Dumont ME. "Genetic interactions among the transmembrane segments of the G protein coupled receptor encoded by the yeast STE2 gene." Journal of molecular biology.. 1997 Feb 28; 266(3):559-75.

Wang X, Dumont ME, Sherman F. "Sequence requirements for mitochondrial import of yeast cytochrome c." The Journal of biological chemistry.. 1996 Mar 22; 271(12):6594-604.

Dumont ME, Corin AF, Campbell GA. "Noncovalent binding of heme induces a compact apocytochrome c structure." Biochemistry.. 1994 Jun 14; 33(23):7368-78.

Dumont ME, Schlichter JB, Cardillo TS, Hayes MK, Bethlendy G, Sherman F. "CYC2 encodes a factor involved in mitochondrial import of yeast cytochrome c." Molecular and cellular biology.. 1993 Oct; 13(10):6442-51.

Dumont, M. E.;. "Mitochondrial Import of Cytochrome c. in: Protein Synthesis and Targeting in Yeast" . (A. Brown J. McCarthy, F. Sherman, and M. Tuite, ed.s) Springer-Verlag, N.Y. 1993; .

Sherman, F.; Moerschell, R.P.; Tsunasawa, S.; Sternglanz, R.; Dumont, M.E. ;. "Co- and Post-Translational Processes and Mitochondrial Import of Yeast Cytochrome c." Translational Regulation of Gene Expression II (J. Ilan, ed.)Plenum Publishing, N.Y. 1993; .

Dumont ME, Cardillo TS, Hayes MK, Sherman F. "Role of cytochrome c heme lyase in mitochondrial import and accumulation of cytochrome c in Saccharomyces cerevisiae." Molecular and cellular biology.. 1991 Nov; 11(11):5487-96.

Dumont MD, Mathews AJ, Nall BT, Baim SB, Eustice DC, Sherman F. "Differential stability of two apo-isocytochromes c in the yeast Saccharomyces cerevisiae." The Journal of biological chemistry.. 1990 Feb 15; 265(5):2733-9.

Dumont ME, Sherman F, Thomas DY. "Protein targeting and protein secretion." Genome. 1989 31(2):1109-10.

Dumont ME, Ernst JF, Sherman F. "Coupling of heme attachment to import of cytochrome c into yeast mitochondria. Studies with heme lyase-deficient mitochondria and altered apocytochromes c." The Journal of biological chemistry.. 1988 Nov 5; 263(31):15928-37.

Dumont ME, Richards FM. "The pH-dependent conformational change of diphtheria toxin." The Journal of biological chemistry.. 1988 Feb 5; 263(4):2087-97.

Dumont ME, Dei-Cas E, Maurois P, Slomianny C, Prensier G, Houcke-Lecomte M, Vernes A, Camus D. "[Histopathology of the liver and kidney during malaria: relation to malaria-induced dyslipoproteinemia]." Annales de parasitologie humaine et comparée. 1988 63(3):171-83.

Dumont ME, Ernst JF, Hampsey DM, Sherman F. "Identification and sequence of the gene encoding cytochrome c heme lyase in the yeast Saccharomyces cerevisiae." The EMBO journal.. 1987 Jan; 6(1):235-41.

Dumont ME, Trewhella J, Engelman DM, Richards FM. "Stability of transmembrane regions in bacteriorhodopsin studied by progressive proteolysis." The Journal of membrane biology.. 1985 88(3):233-47.

Dumont ME, Richards FM. "Insertion of apocytochrome c into lipid vesicles." The Journal of biological chemistry.. 1984 Apr 10; 259(7):4147-56.

Dumont ME, Wiggins JW, Hayward SB. "Location of platinum binding sites on bacteriorhodopsin by electron diffraction." Proceedings of the National Academy of Sciences of the United States of America.. 1981 May; 78(5):2947-51.